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LAB DIAGNOSIS OF ENTEROBACTERIACEAE
Midterm Medical Microbiology 2010
18
Microbiology
Undergraduate 3
03/29/2010

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Cards

Term
IMViC
Definition
INDOLE
METHYL RED
VOGUES-PROSKAUER
CITRATE
Term
INDOLE
Definition
DETECTS TRYPTOPHANASE
POSITIVE RED RING
Term
METHYL RED
Definition
DETECTS PROD OF LARGE AMTS OF STRONG ACIDS VIA MIXED ACID PATHWAY
PH INDICATOR -METHYL RED
POSITIVE- RED COLOR
Term
VOGUES PROSKAUER
Definition
DETECTS PROD OF ACETYLMETHYL CARBINOL (ACETOIN) FROM GLUCOSE FERMENTATION
KOH & ALPHA NAPHTHOL ADDED - RED COLOR =POSITIVE
Term
CITRATE
Definition
DETECTS ABILITY TO UTILIZE CITRATE AS SOLE CARBON/NITROGEN SOURCE
+MEDIA TURNS FROM GREEN TO BLUE
Term
PDA (PHENYLALANINE DEAMINASE)
Definition
ENZYME SPLITS PHENYLALANINE TO PRODUCE PHENYLPYRUVIC ACID
SLANT MEDIA
FERRIC CHLORIDE ADDED
MEDIUM TURNS GREEN IN POS
Term
UREA
Definition
DETECTS UREASE WHICH SPLITS UREA PRODUCING CO2 AND AMMONIA
AMMONIA REACTS WITH WATER FORMING AMMONIUM HYDROXIDE (ALKALINE) TURNS PH INDICATOR (PHENOL RED) TO PINK =POS
Term
ONPG = ORTHONITROPHENYLGALACTOSIDE
Definition
DIFFERENTIATES BTWEEN LACTOSE/NON LACTOSE FERMENTERS
DETECTS ENZYME BETA GALACTOSIDASE
YELLOW=POS
Term
TSIA TRIPLE SUGAR IRON AGAR
Definition
SUGARS - GLU, LAC, SUC
FERMENT GLU - K/A
FERMENT GLU/LAC &/OR SUC - A/A
BLACK PRECIPITATE- H2S POS
AIR POCKETS/CRACKS - GAS POS
Term
5% SHEEP BLOOD AGAR
Definition
DETECTS HEMOLYSIS
ALLOWS GROWTH OF MOST BACTERIA
ALLOWS OXIDASE TESTING
Term
CHOCOLATE
Definition
HEMOLYZED BLOOD
CONTAINS HEMIN AND FACTORS REQUIRED BY CERTAIN ORGANISMS (IE HAEMOPHILUS, NEISSERIA)
Term
MACCONKEY AGAR OR MAC
Definition
SELECTIVE & DIFFERENTIAL
INHIBITS GRAM POS ORGANISMS
BILE SALTS
CRYSTAL VIOLET
NEUTRAL RED AS INDICATOR
Term
MACCONKEY SORBITOL AGAR SMAC
Definition
SELECTIVE AND DIFFERENTIAL
ISOLATE E.COLI 0157:H7 FROM STOOL SAMPLES
LACTOSE REPLACED W/ D-SORBITOL AS PRIMARY CARBO
Term
EMB AGAR EOSIN-METHYLENE BLUE
Definition
SELECTIVE AND DIFFERENTIAL
ISOLATE ENETERIC BACILLI
DIFFERENTIATES LACTOSE AND NON-LACTOSE
PEPTONE BASE WITH LACTOSE AND SUCROSE
INDICATORS: EOSIN AND METHYLENE BLUE
Term
HEKTOEN-ENTERIC (HE) AGAR
Definition
DIFFERENTIAL AND SELECTIVE
ISOLATES SALMONELLA AND SHIGELLA FROM OTHER ENTERICS
PEPTONE BASE
BILE SALTS
LACTOSE
SALICIN
FERRIC AMMONIUM CITRATE
INDICATORS - BROMTHYMOL BLUE AND ACID FUCHSIN
Term
XYLOSE LYSINE DESOXYCHOLATE (XLD) AGAR
Definition
SELETIVE AND DIFFERENTIAL
ISOLATION OF SALMONELLA AND SHIGELLA FROM OTHER GNR
YEAST EXTRACT
LYSINE, XYLOSE, LACTOSE, SUCROSE
FERRIC AMMONIUM CITRATE
INHIBITS GRAM POS BACTERIA
SODIUM DESOXYCHOLATE
PHENOL RED INDICATOR
Term
SALMONELLA-SHIGELLA (SS) AGAR
Definition
SELECTIVE FOR SALMONELLA AND SHIGELLA
PEPTONE BASE
LACTOSE
FERRIC CITRATE
SODIUM CITRATE
NEUTRAL RED INDICATOR
INHIBITS COLIFORMS - BRILLANT GREEN AND BILE SALTS
Term
CEFSULODINSULODIN-IRGASAN-NOVOBIOCIN AGAR
Definition
SELECTIVE FOR YERSINIA SPP
MAY ALSO ISOLATE AEROMONAS
PEPTONE BASE
YEAST EXTRACT
MANNITOL
BILE SALTS
INDICATORS - NEUTRAL RED/CRYSTAL VIOLET
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