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Lab Counts
Hematology
16
Biology
Undergraduate 4
11/06/2011

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Term
WBC Count
Definition
= The total number of cells counted in 9 squares + 10% x 100
Units are cells/mm3
Term
Corrected WBC Count
Definition
Necessary when you see 5+ nucleated RBC’s (NRBC’s)
= The uncorrected WBC count x 100 / The number of NRBC’s you counted during your count + 100
Term
RBC Count
Definition
= The total number of cells counted in 5 smaller squares within the center square x The dilution factor x (1 / Volume)
The units are cells/mm3
Decreased in anemia, acute leukemia, myelodysplastic anemia, hemorrhages, and hemolysis
Term
Platelet Count
Definition
= The total number of platelets in the large center square x 1000
Increased in postsplenectomy, hemorrhage, iron deficiency anemia, and myeloproliferative disorder
Decreased in idiopathic thrombocytic purpera, aplastic anemia, myelodysplastic anemia, and acute leukemia
Term
Hemoglobin Count
Definition
Measured using cyanomethemoglobin
Term
Hematocrit
Definition
Measurement of the packed cell volume
Determined on instruments using RBC counts and the MCV
Done in a centrifuge
-10,000 – 15,000g for 5 minutes
Should be 3x the Hgb result
Term
MCV
Definition
Hct (%) / RBC count x 10
Determines the average volume of RBC’s
Term
MCH
Definition
Hgb (g/dL) / RBC count x 10
The mean of the amount of Hgb in the RBC’s
Term
MCHC
Definition
Hgb (g/dL) / Hct (%) x 100
Average amount of hemoglobin in a dL of RBC’s
Term
Sed Rate
Definition
Rate at which RBC’s settle from plasma
Nonspecific test for inflammation
Term
C Reactive Protein
Definition
Replacing sed rates
Increases before sed rates
Gives you a quantitative result that can be used to monitor rheumatoid arthritis
Term
Retics
Definition
RBC’s with residual RNA
Stained with methylene blue
Term
Raw Retic Count
Definition
Count retics in 5 fields of 200 RBC’s
-Number of retics / 10 = % Retics
Term
Solubility Test for Hemoglobin S
Definition
Determines if someone has hemoglobin S (aka Sickle-cell anemia)
Abnormal hemoglobin that causes sickling of the RBC’s
The blood is mixed with sodium dithionite
-If the solution is clear, the test is negative
-If the solution is turbid, the test is positive
Term
Estimated WBC Count
Definition
= (# of WBC’s counted in 5 fields / 5) x (.2 x109/L)
Fields should be counted at 10x
Term
Estimated Platelet Count
Definition
= (# of platelets counted in 5 fields / 5) x (15 x 109/L)
Fields should be counted at 100x
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