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= The total number of cells counted in 9 squares + 10% x 100 Units are cells/mm3 |
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Necessary when you see 5+ nucleated RBC’s (NRBC’s) = The uncorrected WBC count x 100 / The number of NRBC’s you counted during your count + 100 |
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= The total number of cells counted in 5 smaller squares within the center square x The dilution factor x (1 / Volume) The units are cells/mm3 Decreased in anemia, acute leukemia, myelodysplastic anemia, hemorrhages, and hemolysis |
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= The total number of platelets in the large center square x 1000 Increased in postsplenectomy, hemorrhage, iron deficiency anemia, and myeloproliferative disorder Decreased in idiopathic thrombocytic purpera, aplastic anemia, myelodysplastic anemia, and acute leukemia |
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Measured using cyanomethemoglobin |
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Measurement of the packed cell volume Determined on instruments using RBC counts and the MCV Done in a centrifuge -10,000 – 15,000g for 5 minutes Should be 3x the Hgb result |
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Hct (%) / RBC count x 10 Determines the average volume of RBC’s |
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Hgb (g/dL) / RBC count x 10 The mean of the amount of Hgb in the RBC’s |
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Hgb (g/dL) / Hct (%) x 100 Average amount of hemoglobin in a dL of RBC’s |
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Rate at which RBC’s settle from plasma Nonspecific test for inflammation |
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Replacing sed rates Increases before sed rates Gives you a quantitative result that can be used to monitor rheumatoid arthritis |
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RBC’s with residual RNA Stained with methylene blue |
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Count retics in 5 fields of 200 RBC’s -Number of retics / 10 = % Retics |
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Solubility Test for Hemoglobin S |
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Determines if someone has hemoglobin S (aka Sickle-cell anemia) Abnormal hemoglobin that causes sickling of the RBC’s The blood is mixed with sodium dithionite -If the solution is clear, the test is negative -If the solution is turbid, the test is positive |
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= (# of WBC’s counted in 5 fields / 5) x (.2 x109/L) Fields should be counted at 10x |
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= (# of platelets counted in 5 fields / 5) x (15 x 109/L) Fields should be counted at 100x |
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