Shared Flashcard Set

Details

Lab 9
TPI Assay
14
Biology
Undergraduate 3
03/16/2012

Additional Biology Flashcards

 


 

Cards

Term
Metabolic reactions- speeding up breakdown
Definition
Catabolism
Term
Metabolic reactions- speeding up the synthesis of important compounds
Definition
Anabolism
Term
Enzymes must bind to _______ and activate these chemicals
Definition
Substrates
Term
Enzymes increase the likelihood that a reactive compound will...
Definition
encounter one another.
Term
Enzymes increase the likelihood that a reactive compound will...
Definition
encounter one another.
Term
Enzymes have no effect on the ______ of a reaction, only the ______.
Definition
Equilibrium, speed
Term
What do biologist use to quantify the action of most enzymes?
Definition
Assays, which measure the ability to measure some product of a biochemical pathway.
Term
Test for the enzyme TPI which stands for
Definition
Triose Phosphate Isomerase
Term
How do you test the presence of TPI?
Definition
Coupling it to NADH oxidation.
Term
TPI is involved in what two cellular events?
Definition
Glycolysis and the Calvin Cycle
Term
TPI couples what reaction?
Definition
G-3-P --> DHAP
Term
DHAP + NADH --->
Definition
Glycerol-3-phosphate + NAD+
Term
Adding NADH is used to make
Definition
triglycerides
Term
Experimental question
Definition
which cellular fraction contains TPI?
Supporting users have an ad free experience!