pacemacker cell for intestines

creates slow wave potential

depolarization causes phasic AP firing

There is an excitatory effect on GI motility

increases GI tract contraction

It has a stimulatory effect on GI contractions.

increases GI tract contractions

It prevents the Ca²⁺ myocyte contraction

decreases GI tract contraction

Get 1" segment of rat intestine

Attach each end of the intestine with hooks, 2-3 mm away from the end of the intestine

Keep both strings as short as possible

Maintain appropriate tension on the tissue to obtain a signal on the screen

Maintain temp close to 37^oC

Oxygenate the Tyrode's solution constantly, but not vigorously! (it will create noise)

Keep tissue completely submerged in Tyrode's solution at all times