Term
| Allows the researcher to explore all areas of genetics including: DNA sequencing, gene activation, manipulation and recombination. |
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Definition
| Recombinant DNA technologies |
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Term
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Definition
| Step one of purification, breaks apart cells to liberate DNA from nucleus. |
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Term
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Definition
| buffer- EDTA (chelates calcium) Detergent- SDS (causes membrane holes to open and burst). |
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Term
| Removal of RNA and proteins |
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Definition
| Step 2 for DNA purification. Removing RNA. Add enzyme Rnase A, fragmenting RNA. |
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Term
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Definition
| ammonium acetate, causes proteins to precipitate out while DNA remains aq. |
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Term
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Definition
| requires the addition of isopropanol and enthanol to remove salts. DNA is a white pellet. |
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Term
| How to determine size of DNA fragments |
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Definition
| gel electrophoresis, using a 'gel box'. Charged particles move on a generated electric field within the slab of agarose. |
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Term
| Power supply runs from the negative electrode (_____) to the positive one |
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Definition
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Term
| At the pH we will be using in lab (8.0) the DNA and RNA are _____ charged |
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Definition
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Term
Agrose is a _______, where ____ particles are moving much faster than _____ ones.
conclude- |
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Definition
polysaccharide, smaller, larger.
smallest pieces at the end of the gel. |
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