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Kirby Bauer
Micro lab exam
24
Biology
Undergraduate 3
11/10/2011

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Term
Purpose of Kirby Bauer
Definition
Test to see whether a certain strain of bacteria is susceptible to specific antibiotics (used to determine which antibiotics are best for an infection) 
Term
Modes of action of antibiotics
Definition
  1. Inhibit cell wall synthesis 
  2. Inhibit protein synthesis 
  3. Inhibit nucleic acids
  4. Disrupting cell membrane 
  5. Anti-metabolite 
Term
What type of antibiotic inhibits the cell wall?
Definition
  1. Penecillin 
  2. Ampllcin 
  3. Cephalosporin 
Term
Penecillin
Definition

Targets enzymes that form peptide bridges of peptidoglycan 

 

Term
what types of antibiotics work against gram + bacteria?
Definition
  1. Penecillin
  2. Ampicillin 
  3. cepahlosporin 
Term
What types of antibiotics inhibit protein synthesis?
Definition
  1. Tetracycline 
  2. Streptomycin
  3. Erythomycin
  4. Chloramphenicol
Term
Tetracycline targets
Definition
30s subunit of ribosome 
Term
Streptomycin targets
Definition
gram (-) 
Term
Erythomycin targets
Definition
50s subunit of ribosome 
Term
What type of antibiotics inhibit nucleic acids?
Definition
Quinolones and Rifampin 
Term
Quinolones inhibit
Definition
DNA gyrase that relieves super coil stress 
Term
Rifampin inhibits 
Definition
Bacterial RNA polymerase 
Term
What type of antibiotic disrupts the cell membrane?
Definition
Polymyxin
Term
Polymyxin 
Definition

Act's like a detergent 

  • was originally isolated from (+) bacteria 
  • was against gram (-) bacteria 
Term
What antibiotic inhibits metabolite?
Definition
Sufanethoxzole- trimethopin 
Term
Sufanethoxazole- trimethopin
Definition
prevents folic acid synthesis which is required by some bacteria 
Term
What type of media is used for the Kirby bauer test?
Definition
Mueller Hinton 
Term
What type of media is Mueller Hinton?
Definition
rich nutrient media
Term
what type of bacteria will grow on a mueller hinton?
Definition
allow growth of fastidous bacteria ex. mycobacteria 
Term
what does the mueller hinton media contain?
Definition
beef infusion and casein peptone (it provides a.a. and vitamins)
Term
Do we invert the mueller hinton media plate?
Definition
No inverting!
Term
what is the disadvantage of the kirby bauer test?
Definition
Cannot be used for slow growing bacteria 
Term
Bigger zone inhibition 
Definition
more susceptible to antibiotic 
Term
SIR
Definition
susceptible intermediate resistant 
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