1.      What do the elt genes do? 

They are transcription factors that regulate aging.

1.      Does the evidence from the Kim elt-3 paper indicate that aging is associated with mutation accumulation or an intrinsic developmental pathway of aging?

Developmental pathway

1.      How could the life-span of C. elegans be extended at a molecular level?

     Mutating Daf-2 insulin receptor genes or by continuing expression of elt-3 late in life.

1.      What is RNAi?

  RNA interference that inhibits gene expression

1.      What is the consensus binding site motif of age regulated genes?

    GATA

1.      Is elt expression continuous through time at all positions in the worm and how was this discovered (don’t use acronyms)?

  No, GFP was used (Green Fluorescent Protein)

1.      What prolongs expression of genes that change their expression late in life?

    Elt-3 expression

1.      What does a GATA motif do for a gene?

   Binds transcription factors that are responsible for age related changes in gene expression.

1.      How does age-1 normally function in relation to elt-3 (or what does an RNAi mutant for age-1 change expression of elt-3)?

Age-1 represses elt-3 expression

1.      What does age-1 normally do for genes that change expression in old age?

   Represses expression

1.      How does elt-5 and 6 expression change through time and how was this studied in the Kim paper?

    It increases later in life and was discovered using Cherry.

1.      How would elt-3 expression change in elt-5(RNAi) and elt-6(RNAi) fed worms?

   It would increase

1.      How does elt-5 RNAi or elt-6 RNAi affect lifespan of worms?

Increases lifespan (WT decreases lifespan)

1.      Graphically show the relationship between elt5, and 6 to elt-3 over the life span of worms.