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1. a piece of DNA was intorduced into the nucleus of the cell 2. DNA was transcribed into single-stranded RNA which matched with complementary RNA and necame double-stranded RNA 3. the double stranded RNA was recognized by the proteins (cops) 4. RISC (the cop) caused the mRNA for purple pigment to be destoryed 5. there were no purple pigment mRNAs for the ribosomes to translate so the flower cells remained white |
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-can cause cellular mRNA to be destoryed -adding RNAi targeted to the purple gene causes translation of purple pigment mRNA to stop |
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-the core of RNAi technology is formation of a .... -what does ---- destory? |
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insert a DNA gene to make the RNA |
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-done through in vitro--out of body -remove cells from patients body -make appropriate piece of recombiant DNA -allow vector to deliver DNA to cells -replace cells in patients body -promoter and RNAi gone -rDNA is put into the chromosomes and make RNAi by transcription |
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-in vivo--in body -make appropriate kind of recombiant DNA -give the patient the vector -allow vector to deliver DNA to the cells -most common approach |
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dominant disorders is treated.... |
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recessive disorder treated by... |
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-gene therapy -involves increasing amount of specific mRNA inside specific cells in patients body -gene replacement |
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mutations that cause cancer turns on gene to cause cell to divide. What is most likely to prevent uncontrollable dividing? |
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