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Intro to Microbiology-Cadenas
Final Exam: control of M/Os, M/Os and Disease
51
Microbiology
Undergraduate 1
07/22/2011

Additional Microbiology Flashcards

 


 

Cards

Term

 

Name the 3 types of

Microbial Control Methods

Definition


Physical Agents

Chemical Agents

Mechanical Removal Agents

Term

 

What applications are involved in the use of Physical Agents?

Definition

 

Heat

or

Radiation

Term

 

What applications are involved in using Chemical Agents?

Definition

 

Gases

+

Liquids

Term

 

What application is involved in using Mechanical Agents?

Definition

 

Filtration

Term

 

Who is responsible for choosing appropriate microbial control methods?

Definition

 

Microbial Control

Method Officer

Term

True or False

 

Microbial control methods eradicate microbes.

Definition

False: they reduce the number of microbes, and

may eradicate, but they

don't always.

Term

True or False

It's harder to kill spores than to kill vegetative forms.

Definition

 

True

Term

 

To kill endospores

apply moist heat at

120° or 100ºC?

 

Definition

 

Endospores killed at 120ºC

with moist heat.

Heat is a physical control method.

Term

To kill vegetative forms

apply moist heat at

80° or 100ºC?

Definition

 

Vegetative forms are killed at 80ºC using moist heat.

Heat is a physical control method.

Term

To kill endospores

apply 4,000 or 5,000

grays of radiation?

 

Definition

 

Endospores are killed

using 4,000 grays of radiation.

 

Term

To kill vegetative forms

apply 1,000 or 1,500

grays of radiation?

 

Definition

 

Vegetative forms are killed with 1,000 grays of radiation.

Radiation is a physical killing agent.

Term

To kill endospores

apply 1,000 mg/l or

1,200 mg/l of sterilizing gas?

What IS the gas used?

 

Definition

Endospores are killed by

 applying 1,200 mg/l of sterilizing gas (ethylene oxide). (remember ethyl alcohol??)

 

Term

To kill vegetative forms

 apply 900 mg/l or

700 mg/l of sterilizing gas?

What IS the gas used?

 

 

 

Definition

Vegetative forms are killed by

 applying 700 mg/l of sterilizing gas (ethylene oxide). (remember ethyl alcohol??)

Term

Endospores are killed after 3 days or 3 hours of exposure to sporicidal liquid?

>>Name the liquid.

Definition

Endospores are killed after 3 hours of exposure to a sporicidal liquid:

2% glutaraldehyde.

Term
How long does it take a sporicidal iquid to kill vegetative forms?
Definition

 

10 minutes

(Sporicidal liquids are chemical agents.)

Term

 

Killing endospores and vegetative cells is an art.

True or False?

Definition

 

False - killing endospores and vegetative forms is a SCIENCE.

Term

 

Cadenas statement about use of control agents

and bacterial resistance:

Definition

 

 

Higher resistance by endospores compared to vegetative forms is an argument for needing more Microbial Control Method Officers.

 

Term

 

Define

STERILIZATION

Definition

Book: a process that destroys or removes all viable microorganisms, including viruses.

Cadenas: one should ask, "If not destroyed, where have the M/Os been moved, and where are they now?"

Term

 

Define

STERILE

Definition

Book: any material that has been subjected to the process of destruction or removal of all viable microorganisms, including viruses.

Cadenas: THIS IS STRAIGHTFORWARD

Term

 

Define

BACTERICIDE

 

Definition

Book: a chemical that destroys bacteria except for those in the endospore stage. It may or may not be effective on other microbial groups.

Cadenas: no further comment


 

 

Term

 

Chopping up a capsid to destroy a virus is using what method of microbial control?

Definition

 

 

STERILIZATION DESTROYS OR REMOVES ALL VIABLE M/Os - this includes viruses. 

 

 

 

 

 

 

 

 

THIS WAS MENTIONED DURING SECTION ON DEFINITIONS. Chopping up viral capsids is a way to destroy viruses.

 

Term

 

Define

FUNGICIDE

Definition

Book: a chemical that CAN kill fungal spores, hyphae and yeasts.

 

Cadenas: THIS IS NOT LIMITED TO FUNGI, PER THE DEFINITION. USE OF THE WORD CAN INDICATES THAT IT'S NOT ALWAYS EFFECTIVE.

 

Term

 

Define

VIRUCIDE

Definition

Book: any chemical known to inactivate viruses, especially on living tissue.

 

Cadenas: THE VIRUSES ARE NOT KILLED BECAUSE VIRUSES ARE NOT ALIVE.

 

Term

 

Define

BACTERIOSTATIC

Definition

Book: an agent which prevents the growth of bacteria on tissues or on objects in the environment.

 

Cadenas: STASIS = STOPPING/CEASING OF MOTION/TO STAND STILL. **this is a BACKUP MECHANISM used because a bactericide may not have killed all the bacteria. This would be Step 2.

 

Term

 

Define

DISINFECTION

Definition

Book: the use of a physical process or a chemical agent (a disinfectant) to destroy vegetative pathogens but not bacterial endospores. (...usually only on inanimate objects)

 

Cadenas: WOULD NEED TO FOLLOW UP W/ANOTHER METHOD TO TAKE CARE OF SPORES LEFT BEHIND.

 

Term

 

What is a

VEGETATIVE PATHOGEN?

Definition

 

Cadenas note:

veg. pathogen is a fully-grown disease-causing pathogen.

Term

 

Define

SEPSIS

Definition

Book: the growth of microorganisms in the blood and other tissues.

 

Cadenas: IMPORTANT TO REMEMBER THAT SEPSIS IS NOT LIMITED TO BLOOD. One should ask,"How far has sepsis progressed? New/lingering infection? Any other tissues involved?"

 

Term

 

Define

ASEPSIS

Definition

Book: any practice that prevents the entry of infectious agents into sterile tissues and thus prevents infection.

 

Cadenas: THIS IS PREVENTATIVE, WHICH IS A MAJOR FOCUS IN HEALTHCARE.

 

Term

 

Define

"ZANITIZATION"

Definition

(just a "Cadenas-ism")

Sanitization: any cleansing technique that removes debris, microorganisms, and toxins, and in this way reduces potential for infection and spoilage.

 

Cadenas: SPORES ARE INCLUDED IN THIS! DEBRIS = food for M/Os.

 

Term

 

3 Cadenas suggestions re: qualities of chemical agents used by healthcare workers...

Definition
  1. Killing spores ≠ always killing bacteria.
  2. You should monitor drugs being removed from market, has legal and health implications.
  3. Know drugs you are administering, you'll need to monitor therapeutic levels.
Term

 

 

CHLORINE

 

Definition

 

 

Microbes targeted:   SLOW sporicidal

Leval of Activity:      Intermediate

Toxicity:                  ↑ toxic gas (vapors), skin irritant

             Inactivated by organics, unstable in sunlight.

Cadenas: NOT bacteriostatic or bactericidal AT ALL. Can introduce problems due to cracks in skin. Strong enough to kill spores ≠ strong enough to kill bacteria.

 

 

Term

 

 

IODINE

Definition

Microbes targeted:   SLOW sporicidal

Leval of Activity:      Intermediate

Toxicity:                  Tissue irritant, Toxic to ingest


Cadenas: NOT TO BE LEFT IN ROOM WITH PATIENTS OF ANY AGE. Also, many believe that if it hurts, it must be clean!

Term

 

ALCOHOL

Definition

Microbes targeted:   Most bacteria, viruses, fungi

 

Leval of Activity:      Intermediate

 

Toxicity:                  Toxic to ingest, mild irritant, dries skin

 

                              also Flammable, fast-acting

 


Cadenas: no comment

 

Term

 

SOAPS

Definition

Microbes targeted:   Certain very sensitive species

 

Leval of Activity:      VERY LOW

 

Toxicity:                  Nontoxic, few if any toxic effects

 


 

Cadenas: USED FOR REMOVING SOIL, OIL, DEBRIS. More effective soaps lift both + and - charged items from surface or skin.

 

Term

 

MERCURIALS

Definition

Microbes targeted:   WEAKLY microbistatic

 

Leval of Activity:      LOW

 

Toxicity:                  HIGHLY Toxic to ingest, inhale, absorb

 


 

Cadenas: USED OUTSIDE OF THE U.S. THESE DAYS. This does not kill ANYTHING, and barely even slows down the M/Os.

 

Term

 

GLUTARALDEHYDE

Definition

Microbes targeted:   Sporicidal

 

Leval of Activity:      HIGH

 

Toxicity:                  Can irritate skin, Toxic if absorbed

 


 

Cadenas: EMPHASIZED HIGH LEVEL OF TOXICITY IF ABSORBED.

 

Term

 

TRUE OR FALSE

Soaps kill microorganisms.

Definition

 

 

 

 

 

 

 

 

 


 

 

 

False:

soaps only lift surface debris, etc. from skin.

Only added antimicrobial chemicals would kill some M/Os.

 

Term

SOAPS:

cationic soaps are

preferable why?

Definition

Cationic soaps/detergents have:

 

 

 

 


molecules with a + charged end

(binds with - charged bacterial surface proteins)

and

an uncharged hydrocarbon chain which

helps lyse M/O cell membrane.

(p. 343)

Term

>>>we have to know ALL OF TABLE 12.1 ON P. 352

 

characteristics of the

 IDEAL ANTIMICROBIAL DRUG

Definition
  1. Selectively toxic to _______ but nontoxic to _____.
  2. Microbicidal rather than microbi________.
  3. Relativley soluble; functions even when highly diluted in ________ fluids.
Term

>>>we have to know ALL OF TABLE 12.1 ON P. 352

 

characteristics of the

 IDEAL ANTIMICROBIAL DRUG

(continued)

Definition

4. Remains potent long enough to act and is not _________ or _______ prematurely.

5. Doesn't lead to the development of _______. (Hint: Cadenas also stated that we don't want drug to allow Transformation.)

Term

>>>we have to know ALL OF TABLE 12.1 ON P. 352

 

characteristics of the

 IDEAL ANTIMICROBIAL DRUG

(continued)

Definition

6. Complements or assists the activities of the host's ___.


7. Remains active in tissues and _____.

8. Readily delivered to the site of _____.

Term

>>>we have to know ALL OF TABLE 12.1 ON P. 352

 

characteristics of the

 IDEAL ANTIMICROBIAL DRUG

(continued)

Definition

9. Reasonably priced.


10. Does not disrupt the host's health by causing ____ or predisposing the host to other ____.

Term

Name the 5 mechanisms for Antimicrobials

(the targets for these drugs)

Definition

1) inhibition of cell wall synthesis

2) breakdown of the cell membrane structure or function

3) inhibition of structures and functions of DNA and RNA

4) inhibition of protein synthesis

5) blocks on key metabolic pathways (via enzymes)

 

 

 

 

Term

 

What is the

Therapeutic Index?

Definition

 

The measure (an indicator)

of a drug's toxicity

Term

 

Therapeutic Index

 Is a TI of 10 better

than a TI of 1.1?

Definition

a TI of 10 is much better. A lower ratio such as 1.1 indicates that the

MINIMUM effective (therapeutic dose)

is very close to the toxic dose

Term

 

Define

CARRIER

Definition

Book: An individual who inconspicuously

shelters a pathogen and spreads

it to others without any notice.

Term

 

Define

VECTOR

Definition

Book: a live animal that transmits an infectious agent from one host to another

Term

 

Define

ZOONOSIS

Definition

Book: an infection indigenous to animals but naturally transmissible to humans.

Term

 

Define

COMMUNICABLE

Definition

Book: describes disease when an infected host can transmit the infectious agent to another host and establish infection in that host

Term

 

Define

CONTAGIOUS

Definition

Book: when an infectious agent is highly communicable, especially through direct contact

Term

 

Define

NOSOCOMIAL INFECTION

Definition

Book: infectious diseases that are acquired or develop during a hospital stay

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