Term
how long does it take the body to detect the first response to an infection?
second response? |
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Definition
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Term
Active adaptive immunity
Natural:
Acquired:
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Definition
Natural: patient acquired, and recover
Acquired: vaccination |
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Term
passive adaptive immunity
Natural:
Acquired: |
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Definition
Natural:breastmilk
Acquired: patient given pre formed immune components. |
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Term
innate immune system cells |
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Definition
recognize broad paterns of cells using molecular patern recognition receptors
coded in the DNA (so unchangeable)
for example NK recognize by lack of normal expression of molecules. |
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Term
adaptive immune system cells |
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Definition
identify specific antigens. DNA can change.
T cells
B cells |
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Term
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Definition
good immunogens: BIG, proteins, carbs, complex
bad immunogens: small, lipids
Doesn:t matter: charge and shape |
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Term
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Definition
part of the antibody that is reactive(γ-globulin)
1.) they can be immunogenic |
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Term
what are the immunoglobulins in descending order of prevelance? |
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Definition
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Term
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Definition
domain: repeated primary AA regions in heavy and light chains
Region: variable part where it binds to antigens. hypervariable region contacts epitope |
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Term
Secondary antibody response |
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Definition
- quicker
- more intense, more antibodies made
- more prolonged
- primary exposure most common is IGm. secondary exposure most common is IGg, IGa or IGe.
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Term
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Definition
- usually carbs or polymers
- directly bind to Ig antigen receptors and bridge them together
- only IGm has a role
- no memory response
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Term
Titer
Antiserum
Antiglobulin
Precipiten
Agglutimin
Hemolysis |
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Definition
Titer: inverse of highest dilution to illicit a response
Antiserum: serum that has antibodies
Antiglobulin: part of serum protein with antibody reactive against an antigen
Precipitin: an antibody that precipitates when it encounters an antigen
Agglutimin: an antigen that reacts with a cellular insoluble antigen resulting in clumping
Hemolysis: an antibody that causes lysis of a RBC
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Term
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Definition
Affinity: sum of atractive and repulsive forces between AB and AG
Avidity: strength of binding of AG to AB |
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Term
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Definition
- AG is incubated in plate, some stay on
- plate washed, some G remain
- excess binding sites are blocked by proteins
- patient diluted blood added to plate, free AB washed away
- secondary anti-immunoglobulin AB coupled to an enzyme is added, binds to patient AB, free AB washed away
- bound AB is detectedby dye
- light determines concentration
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Term
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Definition
- separate proteins and denature. put on gel for elecrofloresis to determine protein size
- transfer proteins from gell to immobalizing nitrocellulos membrane, electrical charge done.
- excess proteins/AG binding sites on mitrocellulose membrane are blocked.
- overlay mitrocellulose membrane with patient specimin containing AB
- detect bound AB using elisa
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Term
Secondary binding test
Precipitation |
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Definition
ouchterloney. patient sample loaded into gel matrix in dish, AB loaded nearby. diffusion patern determines. can find fungus disease
radial immunodiffusion test: known AB is mixed in gel matrix, AG loaded into punched holes in gell. diffusion occurs, rings happen. bigger ring, more AG. can find isotype humoral immune deficiency
Immunoelectrophoresis: serum in punched holes, elecrophoresis occurs. good to determine gammopathies multiple myelomas. |
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Term
secondary binding test
direct agglutination
Pasive agglutination |
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Definition
direct agglutination: AB and AG comine, clumping happens. good for blood typing
Pasive agglutination: known AB or AG bound to insoluble item like latex bead. combbine with serum, see clumps. |
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Term
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Definition
- opsonins C3b and C4b tag things to be phagocitized
- recruit leukocites to an area(chemotaxis)
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Term
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Definition
C1INH: binds to C1r and C1s causing them to dissociate from C1q. also prevents spontanious C1 activation
DAF CD55: acts to dissociate C4b2b to inhibit the clasical pathway
Factor I: cleaves C4b following dissociation of C4b2b by DAF
Factor H: compete with factor B for binding to surface boud C3b. inhibits the alternative pathway
Vitronectin: inhibits insertion of MAC, prevents cytolysis
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Term
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Definition
deficient in C1,4,2,3 or I: more suseptible to infection by encapsulated bacteria, dificulty clearing immune complex. impaired clasical pathway
deficient in C5,6,7,8: trouble handling blood born infections
Type I heredetary angiodema: low on C1 esterase inhibitor results in spontanious edema.
Factor H deficient: lysis of RBC by alternative pathway
Properdin deficient: defect in alternative pathway, infections by encapsulated bacteria
MBL deficient: more suseptible to many bacteria. |
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Term
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Definition
- Non professional
- kill pathogens in extracellular by releasing lysosomes
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Term
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Definition
- Non-professional
- kill extracellular
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Term
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Definition
- professional
- major type of myeloid cell
- rabbit
- kill intracellularly or extracellulrly
- chemotherapy drugs lower neutrophils.
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Term
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Definition
- professional phagocyte
- monocytes in blood, macrophage in tissue
- tortoise
- can perform antigen presentation to T cells.
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Term
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Definition
1.) Chemotaxis: phagocytes follows chemicals from compliment to a site
2.) Adherance: at the site, opsonin helps phagocyte bind to material, by C3b and iGg. C3b+IGg=synergize.
3.) Ingestion: phagosome surounds victim
4.) Destruction: digest/killing |
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Term
leukocytes traveling in blood vessels |
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Definition
1.) weak binding step allows rolling adhesion
2.) tight binding that arrests rolling adhesion and allows WBC to squeeze between endothelial cells (diapedesis) |
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Term
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Definition
inflamation due to relaxation of vascular semmoth muscle and vasodilation |
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Term
on sensing microbial production, macrophages ecrete |
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Definition
IL-1β: activates vascular endothelium, lymphocytes, local tissue distruction, increase access of effector cells. fever, production of IL-6
TNF-α: activate vascular endothelium, increase vascular permiability, more IGg/complement/cells into tissue. fever, mobalize metabolites,shock
IL-6: activate & increase AB production. fever, increase acute phase protein production. regulates Albunin, fibrinogen, hemopexin, cysteine protease inhib.
IL-8: chemotactic factor, recruits WBC, activation
IL-12: activate NK cells, induce differentiation of CD4 T cells into TH1 cells |
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Term
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Definition
1.) adhesion molecules expressed on vascular endothelium
2.) pavementing and diapedesia
3.) chemotaxis of leukocytes |
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Term
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Definition
administered with immunogen, non specifically enhance immune response.
can prevent dilution/dispersion of the immunogen
cause mild irritation to enhance antigen processing.
(eg) alum.
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Term
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Definition
enzyme that breaks IGg at hinge region into 3 parts.
2 parts (FAB) can still bind
1 part binds to compliment |
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Term
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Definition
mutations in hypervariable region when producing clones of B cells. specific
when the B cells encounters an antigen it can become a pasma cell or a memory B cell. hapens only after mature B cell with IGm and IGd. if before, the B cell is destroyed (tolerance) |
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Term
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Definition
- most common
- neutralize toxins
- opsonization
- compliment
- cross pacenta
- bind 2 antigens at once
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Term
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Definition
- Pentamer
- J chain joins the units together
- Monamer on B cells
- no hinge region, but extra constant domain on heavy chain (4 domains)
- first IG in primary response
- important for compliment
- neutralize toxins
- can bind 5 antigens at once
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Term
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Definition
- secreted as 2 piece dimer linked by J chain made by B cells. serum as monomer
- has secretory component produced by epithelial cells that protects IGa from denature
- external secretion in fluids like milk, tears, saliva
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Term
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Definition
- extra constant heavy doman (4 domains)
- responsible for alergies
- non-aglutinating
- defend from parasites
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Term
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Definition
- 1 less ss bond
- not secreted by plasma cells
- coexpressed with IGm on B cells as antigen receptor
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Term
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Definition
Sensative: find the diseased. good for results that are negative to rule out. many false +. Eliza test. god fro screaning
Specific: exclude the non diseased. helpfull when test is posative to rule in. many false -. western blot. good to confirm. |
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Term
compliment
Clasical activation pathway |
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Definition
C1-> C4-> C2-> C3.
activated by IC containing (2) IGg or (1) IGm.
1.) C1 binds to AG, and gets activated to C1'
2.) C1' atracts, and claves a C4. C4a goes away, C4b binds to membrane
3.) C1' atracts and cleaves a C2. C2a goes away, C2b binds to C4b on surface.
now cell surface is {C4b2b} a C3 convertase
4.) C4b2b cleaves a C3. C3a goes away, C3b binds.
now cell surface is {C4b2b3b} a C5 convertase
terminal pathway occurs
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Term
complement
alternate pathway
(properdin pathway) |
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Definition
functions in absense of AB.
first we need an activator: something that hydrolyses C3
1.) hydrolysis of C3 happens. C3a goes away, C3b binds to cell surface.
2.) factor B comes and binds to C3b on cell surface.
now cell surface is factorB-C3b
3.) factor D comes and breaks factor B, so Ba goes away.
now cell surface is C3bBb
4.) properdin binds C3bBb
now cell surface {properdin-C3bBb} C3 convertase
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Term
Completment
Lectin pathway |
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Definition
identicle to clasical pathway exept MBL is used instead of C1
so MBL, C4, C2, C3 |
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Term
destruction
resperatory burst |
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Definition
1.) cell makes NADPH oxidase
2.) O2 becomes make super-oxide anion O2- which combines with H to make H2O2 which interacts with myeloperoxidase to make hypochalide ion OCl- which kills bacteria
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Term
Destruction
Phagosome-lysosome fusion |
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Definition
O2 independent
lysosome fuses with phagosome (phagolysosome) |
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Term
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Definition
- mark cells for destruction
- goal is to make C3 convertase. C3 has short life, so is rate limiting step
- final goal is to make MAC (straw) so ions go out, water goes into cell
- "anaphylatoxins C5a>C3a>C4a"
- "margination C5a"
- "chemoatractants for WBC C5a>C3a"
- "regulation of vascular tone C2 and C2 Kinin"
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Term
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Definition
C6, C7, C8.-> {C9} group up to make the straw
triggered by C5b |
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Term
C1inhibitor
regulates which pathway?
what if someone lacks it? |
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Definition
Regulates C1 (clasical pathway)
"dissociates C1r and C1s from C1q"
can also disrupt MBL (lectin pathway)
if lacking, "type 1 heredetary angiodema". stress-> vasodilation/adema. cant control vascular tone. overproduction of C2a, C2a kinin |
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Term
Decay activation factor
(DAF)
CD55
what does it regulate?
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Definition
breaks clasical C3 convertase
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Term
Factor I
what does it influence? |
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Definition
cleaves C4b of clasical C3 convertase |
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Term
Factor H
what pathway does it influence?
what happens of deficient? |
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Definition
competes with factor B for binding to C3b
alternate pathway
deficient = lyse RBC, and endothelium
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Term
anaphylatoxin inactivator
serum carboxypeptidase B |
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Definition
removes arg from C3a, C4a, C5a |
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Term
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Definition
binds C5, C6, C7. prevents MAC from forming |
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Term
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Definition
binds C5b-8. prevents C9 from forming (no straw) |
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Term
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Definition
- suseptible to encapsulated bacteria
- poor at clearing IC, so have AID
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Term
deficient in C5->C9
deficient in C5->C8 |
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Definition
C5->C9: can't make MAC
C5->C8: can't kill gram -. meningitis. |
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Term
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Definition
increased infection by encapsulated, meningitis
alternate |
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Term
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Definition
suseptible to many bacteria
Lectin pathway |
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Term
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Definition
Basophil: regulate hypersensitivity
Eosinophil: allergy/parasites |
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Term
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Definition
1.) chemotaxis: C5a, C3a, C4a. chemokines (interleukins). diapedesis happens
2.) Adherance: binding ↑ opsonins. IGg1, IGg3, C3b
3.) Ingestion |
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Term
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Definition
IFNγ made by CD4+ Helpter T cells and NK |
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Term
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Definition
IL1: local tissue distruction, fever, trigger IL6
IL6: fever, induce phase protein, FIBRINOGEN
IL8: NOT ACUTE PHASE RESPONSE. chemotactic, activate WBC
TNFα: vaso permiability, fever/shock. |
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Term
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Definition
1.) rolling: cell adhesion molecules "selectins"
2.) tight bind: ICAM-1 "integrin"
3.) Diapedesis: CD31
4.) Chemotacis |
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Term
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Definition
IL-4: induce growth and isotype switching of IGg1 and IGe
IL-5: turn B cells into plasma cells, and isotype switching ti IGa
IL-6: differentiating factor can be made by macrophages, epithelial, endothelial
IL-13: growth and differentiation of B cells, and isotype switching to IGe. works with IL-4
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