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Immunology Study Questions Part B
Molecular Techniques
39
Biology
Undergraduate 4
09/10/2015

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Cards

Term
Name 3 things that can interfere with the extraction of intact DNA from cells
Definition
Vigorous vortexing
Multiple freeze-thaw cycles
DNAse
Term
Name 3 things that can interfere with the extraction of intact RNA from cells
Definition
Vigorous vortexing
Multiple freeze-thaw cycles
RNAse
Term
Name the 2 sites in a cell that contain genomic DNA
Definition
Mitochondria
Nucleus
Term
The approximate percentage of the human genome that encodes for genese is what percent?
Definition
5%
Term
What determines the rate a DNA fragment moves through a gel during electrophoresis?
Definition
Size
Term
Name 4 characteristics of a nucleic acid probe
Definition
May be RNA or DNA
Detects RNA or DNA
Single stranded oligonucleotide
Complimentary to the target nucleotide
Term
Name the contaminant in a PCR lab most likely to cause erroneous results
Definition
DNA
Term
State the inheritance pattern in humans for the following:
Short Tandem Repeats
Definition
Both Maternal & Paternal
Term
State the inheritance pattern in humans for the following:
Mitochondrial DNA
Definition
Maternal
Term
State the inheritance pattern in humans for the following:
Y Chromosome
Definition
Paternal
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Crithidia
Term
Identify the following:
[image]
Definition
Crithidia
Term
Identify the following:
[image]
Definition
Homogeneous
Term
Identify the following:
[image]
Definition
Homogeneous
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Peripheral (Rim)
Term
Identify the following:
[image]
Definition
Peripheral (Rim)
Term
Identify the following:
[image]
Definition
Peripheral (Rim)
Term
Identify the following:
[image]
Definition
Speckled
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Centromere
Term
Identify the following:
[image]
Definition
Homogeneous
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Speckled
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Nucleolar
Term
Identify the following:
[image]
Definition
Speckled
Term
Identify the following:
[image]
Definition
Speckled
Term
Identify the following:
[image]
Definition
Speckled
Term
Identify the following:
[image]
Definition
Speckled
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