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Immuno 12/12/12 Immune Cytotoxicity
HDPP final at UMMSM
25
Immunology
Graduate
12/13/2012

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Cards

Term
What are CTL-cells?
Definition
Cytotoxic effector CD8 T-cells that eliminate infected cells
Term
What is the job of CD4 TH1-cells?
Definition
Activation of macrophages so they can efficiently phagocytize intracellular pathogens
Term
What can cause type IV hypersensitivity reactions?
Definition
Proteins leading to local skin swelling cause delayed type; haptens or metal ions leading to local epidermal reaction cause type due to contact
Term
What do effector CTL-cells do?
Definition
They have altered levels of adhesion molecules (low L-selectin) which prevents movement into lymph nodes. They enter infected cells via VLA-4 binding to VCAM, causing increased CD2 and LFA-1 leading to cellular interactions
Term
What is required for CD8 T cell activation?
Definition
Help from CD4 TH1-cells, which increase levels of B7 on the APC via CD40L as well as produce paracrine IL-1.
Term
What do active CTL-cells produce?
Definition
IL-2 and high-affinity IL-2 receptors
Term
What mediates the exocytic pathway of killing?
Definition
Granzymes, perforin, and granulysin (cytotoxins) released after CTL-cells rearrange cellular machinery following TCR recognition.
Term
Where do granzymes, perforin, and granulysin act?
Definition
Lytic granules containing cytotoxins are released into the target cell membrane. Perforin and serglycin create pores into which granzyme-B can enter the cell
Term
What signals are required for CTL-cells?
Definition
Cell-cell contact at HLA class I, no second signal
Term
What is apoptosis?
Definition
DNA is fragmented, nucleus is condensed, and plasma membrane forms vesicles for easy phagocytosis without inflammation
Term
What is necrosis?
Definition
Metabolic insults leads to leakage of cellular contents, yielding inflammation and monocyte recruitment
Term
What are caspases?
Definition
Cysteine proteases that cleave proteins at aspartate residues, do not cleave DNA
Term
What is a CAD?
Definition
A caspase activatable DNA-ase found in the cytoplasm of all cells but is inhibited
Term
What does caspase 3 do?
Definition
When activated by granzyme-B, it cleaves the inhibitor (I-CAD) to activate CAD. It also inhibits cellular integrity proteins (lamin B) and nuclear repair enzymes (PARP) and activates endonucleases
Term
How do CTL-cells force death?
Definition
Via "death receptors"--TNF family ligands and transmembrane receptors.
Term
What is the FasL-Fas death pathway?
Definition
Fas and FasL become expressed on activated T cells. Binding of FasL (3 molecules) to Fas initiates an apoptotic pathway: Fas and TNFR1 have "death domain" (DD) which recruits FADD to activate caspases
Term
Who expresses Fas?
Definition
Many cells, especially activated T-cells, some B-cells, but no naive lymphocytes
Term
What mediates extrinsic programmed cell death?
Definition
Granule exocytosis and death receptors.
Term
What can cause intrinsic programmed cell death?
Definition
UV exposure, chemotherapy drugs, starvation, or irreparable genetic damage
Term
How does programmed cell death (PCD) occur?
Definition
Mitochondria are told to release cytochrome c, which binds to Apaf-1 in the cytoplasm, activates cas-9, which activates cas-3 and the caspase chain is initiated
Term
What regulates apoptosis?
Definition
Molecules that promote apoptosis or enhance survival
Term
What is Bcl-2?
Definition
A pro-life molecule that prevents mitochondrial pore formation
Term
What is Bax?
Definition
A death promoter
Term
What is a hallmark of cancer?
Definition
Ability to evade apoptosis
Term
How is Bcl related to cancer?
Definition
Overproduction of Bcl is seen in B-cell lymphomas
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