Term
| What are the specs of a deuterium/hydrogen lamp? |
|
Definition
Range: 160-800nm
Effective range 190-400nm
Excited atoms by electrical discharge cause photon |
|
|
Term
| What are the specs of a tungsten filament lamp? |
|
Definition
Range: 350-2500nm
Eff Rang 350-800nm
Blackbody emitter |
|
|
Term
| What are the specs of a Xenon arc lamp lamp? |
|
Definition
Range 200-1000nm
Eff Range: 200-800nm |
|
|
Term
| How do losses occur regarding the cuvette? |
|
Definition
| Losses occur from reflection and scattering |
|
|
Term
| What is the effect of slit width on peak height? |
|
Definition
| Radiant power is proportional to (slitwidth)^2 |
|
|
Term
| What are the assumptions made by Beer's Law? |
|
Definition
1)A monochromatic beam of light transversing a sample of absorbing molecules loses it intensity exponentially
2)Absorption changes as a fcn of frequency and wavelength
3)Absorption is proportional to conc. (All sp. abs equally)
4)Absorbing substances do not interact w/ ea. other or interfere w/ ea. other's absorption properties |
|
|
Term
|
Definition
| Promotion of compound to higher energy state w/o relaxation |
|
|
Term
| What are limitations on Beer's Law? |
|
Definition
Instrumental deviations
-Stray radiation/light
-polychromatic radiation/light
-mismatched cells
Chemical deviations
-High analyte or low analyte concentrations
-Changes to refractive index of the solvent |
|
|
