Term
| High WBC counts are often observed in ___, ___, and ___ ___ |
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Definition
infections
inflammation
blood malignancies |
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Term
| Infections and inflammation, will see an increase in the number of ______ cells |
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Definition
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Term
| High RBC counts are usually due to ___/____ ____, ____, or a ____ ____ |
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Definition
heart/lung problems
dehydration
myeloproliferative disorder |
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Term
T or F:
All cell lines are increased in Polycythemia |
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Definition
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Term
| High number of Platelets is likely due to ____, _____, or ____ ____ |
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Definition
infections
inflammation
rare malignancy |
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Term
| Low WBC count is due to ____ ___, ____ ___, ____ ____ _ ___, ________ |
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Definition
severe infections
blood malignancies
certain types of anemia
chemotherapy |
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Term
| Low RBC count is due to ___ ___, ___ ___, ____ ____ |
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Definition
Blood loss
Nutritional deficiencies
Inherited conditions |
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Term
| Low Platelet count is due to ___ ___, ___, and ____ ____ |
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Definition
Blood malignancies
chemotherapy
coagulation disorders |
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
T or F:
Newborns have a higher RBC count (5-6 million RBC/µL) |
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Definition
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Term
| Why do males have a higher RBC value? |
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Definition
| Androgen stimulation of erythropoietin that directly stimulates stem cells |
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Term
T or F:
WBC 5.0 x 10^3/µL = 5.0 x 10^9/L for SI |
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Definition
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Term
T or F:
Hematocrit that is 50% is 0.50 L/L (SI) |
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Definition
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Term
| What are the samples for a Manual cell count? |
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Definition
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Term
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Definition
Precisely etched ruled lines forming a grid on each side of the chamber
Allows counts to be performed in duplicate |
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Term
| When performing a hemacytometer count do you need to perform in duplicate AND average your results? |
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Definition
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Term
| Example: Blood is diluted 1:20 for a WBC count. 48 cells are counted on one side and 52 cells are counted on the other side, counting the 4 large corner squares. How would you set up the equation? |
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Definition
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Term
| Example: Blood is diluted 1:20 for a WBC count. 48 cells are counted on one side and 52 cells are counted on the other side, counting the 4 large corner squares. What is the total WBC? |
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Definition
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Term
| Sources of Error in Manual Counting (4) |
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Definition
Inadequate mixing or dilution
Non-random distribution
Observer bias
Incorrect calculation |
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Term
The following are considered ____ of Manual Counts:
Cost is low
Better for counts of less than 100
Automated counts cannot go below 100 |
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Definition
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Term
The following are considered ____ of Manual Counts:
Imprecise
High coefficient of variation
Slow |
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Definition
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Term
| When would a Manual Count be performed? |
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Definition
Calibration of automated methods
Low cell counts
Reference method
Body fluids
High protein in fluid can plug up instrument tubing |
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Term
| Principles of Automated counting (5) |
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Definition
Cell impedance
Current flowing thru conducting fluid via two electrodes
Cells are poor conductors
Interrupt current
Changes in voltage are counted |
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Term
| What 3 things do Coulter counters require in order to perform an automated count? |
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Definition
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Term
The following are all considered:
diluter
computer
pneumatics
electronics |
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Definition
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Term
| Instrument Sources of Error |
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Definition
Artifacts
Anything the same size of a cell will be counted
Incorrect interpretation of data
Not utilizing histograms |
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Term
The following are considered instrument ____:
Improved accuracy and precision
Counts larger number of cells
Low coefficient of variation
Speed |
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Definition
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Term
The following are considered instrument ____:
More problems with artifacts
Anything the size of a cell is counted
Expense
Cannot accurately perform counts on low number of cells
Counts less than 100 |
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Definition
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Term
| What is the corrected WBC formula |
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Definition
| (WBC x 100) / (#NRBC + 100) |
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Term
True or False:
Platelets will not interfere with count for either manual or automated counting |
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Definition
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