Term
| Pluripotential stem cells becomes committed based on ____ ____ |
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Definition
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Term
| Growth factors are usually _______ that provide the stimulus |
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Definition
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Term
| After maturation, the cells remain in the bone marrow for ____ _____ before they are released into the peripheral blood |
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Definition
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Term
| Bone Marrow contains ____ _____ the number of circulating white blood cells |
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Definition
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Term
Which type of cells do the following:
-Marginate into the peripheral blood
---Diapedese into the tissue
-Are stored in the bone marrow until needed in circulation |
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Definition
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Term
| Once Diapedesis occurs do cell return to the blood? |
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Definition
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Term
| After WBC enter the tissue, how long do they live? |
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Definition
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Term
| Cells are removed by which system? |
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Definition
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Term
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Definition
Reduction in size and condensation of chromatin
Nucleus goes from a delicate purple chromatin to clumped chromatin
Nucleoli disappear
Nucleus of myeloid cells changes shape
N:C ratio is decreased (nucleus to cytoplasm) |
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Term
| How many days does Granulopoiesis take to go through the proliferation and maturation? |
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Definition
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Term
| What is the main purpose of the mature neutrophil? |
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Definition
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Term
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Definition
Nucleus is round or oval with abundant, unclumped, light purple reticulated chromatin
2 – 5 nucleoli
Cytoplasm sparse, basophilic
Blue-gray cytoplasm
No granules |
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Term
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Definition
Nucleus is round or oval
Slight chromatin clumping
Nucleoli begin to fade
1 -2 nucleoli
Cytoplasm is more evident than blast
Primary granules are observed
Large non-specific
Red to purple in color
Often are on top of nucleus as well as cytoplasm |
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Term
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Definition
Nucleus is round or oval with chromatin getting coarser
May be slightly flattened on one side
Nucleus may have “dawn of neutrophilia”
Clearing observed near nucleus
No nucleoli are present
Cytoplasm contains:
Primary granules
Secondary granules
Cytoplasm is becoming less blue |
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Term
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Definition
Smaller than myelocyte
Nucleus is kidney-bean shaped
Chromatin becoming coarser
Stains darker
Secondary granules are more evident
Cell is more pink than myelocyte |
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Term
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Definition
Nucleus is horse-shoe shaped and very clumped
“C” or “S” shaped
Pyknotic or clumped ends
>5% in peripheral blood is abnormal
Cytoplasm is abundant with many fine granules
More secondary granules are evident |
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Term
| What is the purpose of a segmented neutrophil? |
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Definition
| Engulf and destroy bacteria |
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Term
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Definition
50-70% circulate in peripheral blood
Slightly smaller than a band
Capable of diapedesis and phagocytosis
Nucleus is pinched into segments connected with a fine filament
Chromatin is coarse
3-5 lobes are observed
Abundant pink cytoplasm with secondary granules |
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Term
| What is the main purpose of the lymphocyte? |
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Definition
| Humoral and cellular Immunity |
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Term
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Definition
Nucleus is round, oval
Highest N/C ratio other than blast
Chromatin is evenly coarse with dark clumping
Nucleolus may be seen
Cytoplasm is blue
May have azurophilic granules
Large red granules |
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Term
| Peripheral blood contains what percentage of lymphocytes? |
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Definition
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Term
True or False:
One Cannot distinguish a B lymphocyte from a T lymphocyte on peripheral blood smear |
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Definition
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Term
| What is the main purpose of the monocyte? |
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Definition
| processing antigens to present to lymphocytes and collect garbage |
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Term
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Definition
Largest cell in normal blood
Nucleus
Indented, folded brain-like convolutions
Delicate, pale chromatin with abundant and distinct open spaces, lace-like
Usually no nucleoli
Cytoplasm is light gray or gray-blue with pseudopods
May contain vacuoles |
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Term
| What is the percentage of monocytes circulating in the peripheral blood? |
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Definition
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Term
| How do you distinguish a monocyte from a band? |
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Definition
The color of the cytoplasm
Monocyte's is a blue-gray |
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Term
| What is the main purpose of the Eosinophil? |
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Definition
| Immunity against parasites, control of inflammation, and allergic responses |
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Term
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Definition
Proliferation sequence same as neutrophil
Cytoplasmic granules are large orange-red
Appear in the myelocyte
Nucleus is band or bi-lobed
Bi-lobed most common
Less than 5% found in peripheral blood |
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Term
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Definition
Involved in cell mediated immunity
Interact with IgE
Same maturation sequence as neutrophil
Granules are huge, dark blue or black
Contain histamine
Granules are so large the nucleus is hidden
Less than 2% are seen in the blood |
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Term
| What is the main function of a Megakaryocyte? |
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Definition
| To make platelets for clotting blood |
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Term
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Definition
Nucleus is multiform with abundant cytoplasm
Very large
Platelet (thrombocyte) pinches off the cytoplasm |
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Term
| What is the main purpose of a Plasma cell? |
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Definition
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Term
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Definition
Nucleus is round and eccentric with clumped chromatin
Cytoplasm is dark blue with pale perinuclear zone
Cornflower blue |
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Term
| What is the purpose of the Macrophage or histiocyte? |
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Definition
| immunity, storage of iron, phagocytosis |
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Term
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Definition
Only seen in bone marrow and tissue
Small nucleus in large amount of cytoplasm
Often observe vacuoles or particle matter |
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Term
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Definition
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Term
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Definition
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Term
| Blood smear should be made in ____ hours |
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Definition
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Term
| Blood smear - ___-____ hours preferred for a smear to be made |
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Definition
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Term
| Blood smear - ____-____ hours ideal for a smear to be made |
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Definition
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Term
| What does making a smear within 5 hours help to prevent? |
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Definition
| Vacuolated, broken WBC; crenated RBC |
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Term
| Wright-Giemsa is what type of stain? |
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Definition
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Term
True or False:
Wright Stain - Alkaline methylene blue stains acidic cellular structures including nuclear DNA and cytoplasmic RNA
Stains blue |
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Definition
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Term
True or False:
Wright Stain - Acidic eosin stains basic hemoglobin and esoinophilic granules
Stains red |
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Definition
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Term
| If the slide of a Wright Stain is too blue what does that indicate? |
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Definition
too much stain
too little buffer
reagents are too alklaine |
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Term
| If the slide of a Wright Stain is too blue what can you do to solve this? |
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Definition
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Term
| If the slide of a Wright Stain is too pink what does that indicate? |
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Definition
Too little stain
Too much buffer
Reagents are too acidic |
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Term
Identify the cell:
N/C Ratio: High
Nucleoli: Yes
Granules: No |
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Definition
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Term
Identify the cell:
N/C Ratio: High
Nucleoli: Yes
Granules: Non-specific |
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Definition
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Term
Identify the cell:
N/C Ratio:Medium
Nucleoli: No
Granules:Specific |
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Definition
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Term
Identify the cell:
N/C Ratio: Low
Nucleoli: No
Granules: Specific |
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Definition
Band Neutrophil
Segmented Neutrophil |
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Term
Identify the cell:
N/C: Low
Nucleus: Clumped, uneven
Cytoplasm: Pink
Granules: Medium, pink
Vacuoles: Maybe |
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Definition
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Term
Identify the cell:
N/C: High
Nucleus: Clumped, even
Cytoplasm: Blue
Granules: Large, red (if present at all)
Vacuoles: Maybe |
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Definition
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Term
Identify the cell:
N/C: Medium
Nucleus: Lacy, even
Cytoplasm: Blue-gray
Granules: Fine, blue or pink
Vacuoles: Likely |
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Definition
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