Term
|
Definition
Clumped of giant platelets
NRBCs |
|
|
Term
|
Definition
| Variant lymphs and blasts |
|
|
Term
|
Definition
| immature granulocytes, eosinophilia |
|
|
Term
|
Definition
| High absolute granulocytes |
|
|
Term
| RBC Spurious Results due to |
|
Definition
| High WBC, RBC fragments, Clumped RBCs as seen in agglutination |
|
|
Term
| MCV Spurious Results due to |
|
Definition
Cold Agglutination (increase)
Tonicity
High WBC |
|
|
Term
| Hgb Spurious Results due to |
|
Definition
High WBC
Lipemia
Icteric sample |
|
|
Term
| What method is used to determine WBC, RBC, and Plt |
|
Definition
|
|
Term
| What method is used to determine Hgb |
|
Definition
|
|
Term
|
Definition
|
|
Term
| Which values are calculated |
|
Definition
|
|
Term
|
Definition
| Cells are gently pulled through the aperture by vacuum |
|
|
Term
|
Definition
| corrects for two cells going through the aperture simultaneously |
|
|
Term
|
Definition
| must agree with two out of three counts to get results |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Cell scatters light dependent on the mass of the cell |
|
|
Term
|
Definition
| uses cell impedance to count WBC and RBC |
|
|
Term
| What does the Coulter Principle count |
|
Definition
|
|
Term
|
Definition
| Red Cell Distribution Width |
|
|
Term
| What does RDW correlate with |
|
Definition
|
|
Term
| If the vacuum decreases, what happens to the cell counts? |
|
Definition
|
|
Term
| What interferes with the Plt count |
|
Definition
| Giant plt, tiny RBCs, RBC fragments, NRBC |
|
|
Term
| What is the NRBC counted as on the 5-part differential histogram? |
|
Definition
|
|
Term
| What effect down platelet satellism have on the platelet count |
|
Definition
|
|
Term
| What parameters are effected by giant platelets? |
|
Definition
|
|
Term
| What is the effect on parameters by giant platelets? |
|
Definition
Increased WBC
Decreased MCV
Decreased Plt count |
|
|
Term
| What is the problem if the WBC is very high and the Hgb is increased |
|
Definition
|
|
Term
| What is the effect of very elevated WBC count |
|
Definition
| Effects RBC, Hgb, Indices |
|
|
Term
| What will a very elevated WBC count not effect? |
|
Definition
|
|
Term
| What happens to the Hgb value when the lamp fades? |
|
Definition
|
|
Term
| If WBC and Plt count are fine, but RBC, Hct, and Indices are decreased? |
|
Definition
|
|
Term
| When do you review a blood smear? |
|
Definition
| Abnormal WBC, RBC, Plt Count |
|
|
Term
| If anemia is present, will the indices and RDW be normal or abnormal? |
|
Definition
| May be either normal or abnormal |
|
|
Term
| What will happen to MCH and MCHC in Lipemic speciments? |
|
Definition
|
|
Term
|
Definition
A: Neutrophils
B: Monocytes
C: Lympocytes
D: Eosinophils
E: Basophils |
|
|
Term
|
Definition
1: Blasts
2: Immature Granulocytes
3: Aged and Damaged Neutrophils
4: Giant Platelets
5: NRBC
6: Lymphocytes
7: Blasts
8: Lymphocytes
9: Blasts |
|
|
Term
|
Definition
1: Fragments
2: MCV
3: Retics
4: Doublets or WBCs |
|
|
Term
|
Definition
1: Bubbles
2: Tiny RBC
3: Large Plts |
|
|
Term
|
Definition
1: Large Plts
2: Lymphs
3: Blasts
4: Monocytes
5: Segs and Eosinophils |
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|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| N/C N/C Anemia with Anisocytosis |
|
|
Term
|
Definition
| Anemia with Hypochromic and Microcytic |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Hypochromic Microcytic Anemia |
|
|
Term
|
Definition
| Severe Anemia with Anisocytosis |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Lymphopenia Granulocytosis |
|
|
Term
|
Definition
| Anisocytosis, Microcytic, Hypochromic, with small platelets |
|
|
Term
|
Definition
| Lymphocytosis Granulocytosis |
|
|
Term
|
Definition
| Sickle Cell Disease most like associated with Thalassemia |
|
|
Term
|
Definition
Autoimmune Disorder Present
-Sjogren's syndrome
-Cold agglutinin syndrome
-Diffuse large cell lymphoma
-Type II cryoglobulinemia |
|
|
Term
|
Definition
Infectious Mononucleosis
Reactive/Atypical Lymphs |
|
|
Term
|
Definition
Dysmyelopoietic Syndrome
- refractory anemia with excess blasts
- giant platelets
- dimorphic rbc
- immature granulocytes |
|
|
Term
|
Definition
| Chronic Lymphocytic Leukemia |
|
|
Term
|
Definition
| Chronic Granulocytic Leukemia |
|
|
Term
|
Definition
| Acute Nonlymphocytic Leukemia |
|
|
Term
|
Definition
Severe Folate Deficiency
-Macrocytosis |
|
|
Term
|
Definition
Severe folate deficiency
- macrocytes
- retics
- left shift |
|
|
Term
|
Definition
|
|
Term
|
Definition
A: Debris
B: Platelets
C: Microcytic RBCs |
|
|
Term
| 4 Advantages of Automation |
|
Definition
Speed
Accuracy
Precision
Small blood volume |
|
|
Term
|
Definition
Cost is low
if low work volume
Extremely small volume of blood
Fewer artifacts and spurious results |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
| Light Scatter Combined with Staining (Abbott) |
|
Definition
| Uses laser counting as well as peroxidase staining for differential |
|
|
Term
| All of the larger analyzers evaluate _________ based on optical scatter or fluorescence after treating RBCs with either fluorescent dyes or nucleic acid stains to stain residual RNA |
|
Definition
|
|
Term
| 5 Components of an Analyzer |
|
Definition
Diluter
DMS
Power Supply
Analyzer
Reagent subsystem |
|
|
Term
| Power supply includes (2) |
|
Definition
|
|
Term
|
Definition
Compressed air that operates pinch valves
Vacuum that pulls cells through orifices |
|
|
Term
|
Definition
| Supplies voltage required for circuitry |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Mixes, transports, pipettes, lyses, senses |
|
|
Term
|
Definition
| Counts, measures, computes the count and size information from the diluter and send to DMS |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Displays, stores, and transmits information to printer |
|
|
Term
| With histograms, all that are under the line are ______ |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
| RBC Histogram particles __ ___ fL in RBC chamber are counted |
|
Definition
|
|
Term
| Platelet Histogram particles between ___ __ ___ fL are considered |
|
Definition
|
|
Term
| Platelet Histogram - process includes are platelets while excluding ___ ___ ___ |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 1 |
|
Definition
| Sample is loaded automatically or manually held |
|
|
Term
| Follow a Drop of Blood Step 2 |
|
Definition
Pump draws a small amount of blood
-usually 100 microliters into BSV |
|
|
Term
| Follow a Drop of Blood Step 3 |
|
Definition
| Sample of blood is picked up by isoton and taken to baths as bubbles come in to mix |
|
|
Term
| Follow a Drop of Blood Step 4 |
|
Definition
| Vacuum draws precise amount of blood/isoton dilution through apeture |
|
|
Term
| Follow a Drop of Blood Step 5 |
|
Definition
| After WBC counted, dilution drops to Hgb cuvette |
|
|
Term
| Follow a Drop of Blood Step 6 |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 7 |
|
Definition
| Results are displayed and a printout is generated |
|
|
Term
| Follow a Drop of Blood Step 8 |
|
Definition
| WBC differentials use various methods |
|
|
Term
| Follow a Drop of Blood Step 9 |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 10 |
|
Definition
|
|
Term
|
Definition
|
|
Term
| RBC bath dilution ____ and _____ are counted |
|
Definition
|
|
Term
|
Definition
|
|
Term
| WBC bath dilution ____ and ____ are determined |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 4 A |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 4 B |
|
Definition
| Cells are pulled through the aperture by vacuum |
|
|
Term
| Follow a Drop of Blood Step 4 C |
|
Definition
| Current is supplied to electrodes |
|
|
Term
| Follow a Drop of Blood Step 4 D |
|
Definition
| Cells passing through cause resistance |
|
|
Term
| Follow a Drop of Blood Step 4 E |
|
Definition
| Resistance Number and Size Sensed |
|
|
Term
| Follow a Drop of Blood Step 4 F |
|
Definition
| Three apertures count for 4 seconds |
|
|
Term
| Follow a Drop of Blood Step 4 G |
|
Definition
| Amplified and displayed by CRT |
|
|
Term
| Follow a Drop of Blood Step 4 H |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 6 i |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 6 ii |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 6 iii |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 6 iv |
|
Definition
| RBC and Plt Histograms complied |
|
|
Term
| Follow a Drop of Blood Step 6 v |
|
Definition
| RBC histograms derives MCV and RDW |
|
|
Term
| Follow a Drop of Blood Step 6 vi |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 2 a |
|
Definition
| Usually 100 microliters into blood sample valve (BSV) |
|
|
Term
| Follow a Drop of Blood Step 2 b |
|
Definition
| Aspiration needle is rinsed with isoton |
|
|
Term
| Follow a Drop of Blood Step 2 c |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 2 d |
|
Definition
|
|
Term
| Follow a Drop of Blood Step 2 ci |
|
Definition
| Blood is segmented into two parts |
|
|
Term
| If calculated Hct parameter is lower than manual count |
|
Definition
|
|
Term
|
Definition
|
|
Term
| RDW is the CV or RBC which equals |
|
Definition
|
|
Term
| Platelets are counted in the same bath as |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Cytoplasm is stripped from all cells except for the basophils |
|
|
Term
| Flow Cytometry - Discriminatory electronic thresholds distinguishes |
|
Definition
| granulated cells from non-staining lymphocytes |
|
|
Term
| Flow Cytometry - __ __ _ ___ _ ___ are utilized to categorize each cell |
|
Definition
| Light scatter and pattern of absorbance |
|
|
Term
| VCS scatterplot incorporated _____ ___ ____ |
|
Definition
|
|
Term
| VCS scatterplot counts _____ WBC/cycle |
|
Definition
|
|
Term
| VCS simultaneously analyzes each cell by (3) |
|
Definition
Volume or size
Conductivity
High frequency electromagnetic probe that measures internal structure |
|
|
Term
| What separates basophils from lymphs? |
|
Definition
| VCS High Frequency Electromagnetic Probe |
|
|
Term
| Which Differential results in a 3-D plot separating all five cell lines? |
|
Definition
|
|
Term
| Which differential provides a good manual differential for normal smears? |
|
Definition
| Coulter Automated Differentials |
|
|
Term
| VCS scatterplot is what type of differential? |
|
Definition
| Coulter Automated Differential |
|
|
Term
| VCS scatterplot provides a good manual differential for ______ ______ |
|
Definition
|
|
Term
| Automated cell counters use either __ __ or ___ ___ ___ |
|
Definition
cell impedance
Optical light scatter |
|
|
Term
| Increased _____ will cause MCV >100 |
|
Definition
|
|
Term
|
Definition
lipemia
icterus
clumped cells |
|
|
Term
| If lipemic or icteric perform |
|
Definition
|
|
Term
| If agglutination is present |
|
Definition
|
|
Term
| 3 possible abnormalities with the R1 flag |
|
Definition
Intracellular parasite
Clumped or Large Plts
NRBCs |
|
|
Term
| 2 possible abnormalities with R2 |
|
Definition
Reactive lymphocytes
blasts |
|
|
Term
| 3 possible abnormalities with R3 |
|
Definition
Eosinophilia
Basophilia
immature neutrophils |
|
|
Term
| Possible abnormality with R4 |
|
Definition
|
|
Term
| Normal RDW is ____ - ____ |
|
Definition
|
|
Term
Normal Hct Values
Male
Female
Children |
|
Definition
|
|
Term
Normal Hgb value
Male
Female
Children |
|
Definition
|
|
Term
Normal RBC count
Male
Female
Children |
|
Definition
4.7-6.1 x 10^6
4.2-5.4 x 10^6
4.4*5.8 x 10^6 |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
