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mean cell volume-indicates RBC size |
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mean cell hemoglobin-weight of hemoglobin contained in RBC |
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mean cell hemoglobin content-average concentration of hemoglobin in RBC |
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Colony-stimulating factor |
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too much stain too little buffer reagents too alkaline |
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too little stain too much buffer reagents too acid |
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4500 to 11500 cells/uL 4.5 x 10^3 - 11.5 x 0^3/uL or mm3 |
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150,000-450,000 cells/uL 150 x 10^3/uL |
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hemacytometer counts calculation |
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cells counted x dilution factor x depth factor/area counted based on 1 large square |
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nucleus-2-5 lobes thin filaments chromatin-coarsely clumped cytoplasm-pale pink, cream, colorless granules-secondary abundant |
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nucleus-constricted, chromatin visible chromatin-coarsely clumped cytoplasm-pale blue to pink granules-secondary abundant |
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nucleus-round to oval, slightly indented chromatin-condensed to deeply condensed cytoplasm-scant to moderate;sky blue granules-maybe few azurophilic |
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nucleus-variable-round to horseshoe or kidney, folded w/brainlike convolutions chromatin-moderately clumped, lacy cytoplasm-blue-gray, maybe pseudopods or vacuoles granules-many fine granules "ground glass" |
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nucleus-2-3 lobes thin filaments chromatin-coarsely clumped cytoplasm-cream to pink, maybe irregular borders granules-abundant red to orange, round secondary |
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nucleus-usually 2 lobes thin filaments chromatin-coarsely clumped cytoplasm-lavender to colorless granules-secondary-lavender to dark purple, uneven dist may obscure nucleus or wash out look like empty areas of cytoplasm |
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platelet estimate w/ anemia |
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avg#platelets/field x total RBC count/200 RBCs/field |
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avg # platelets/field x 20,000 |
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