Term
| What is regenerative medicine? |
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Definition
| The process of replacing, engineering or regenerating human cells, tissues or organs to restore or establish normal function. |
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Term
| What are two theories of aging? |
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Definition
| Programmed theory and error theory |
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Term
| The main features of error theory |
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Definition
Wear & tear theory
Rate of living theory
Cross-linking theory
Free-radicals theory |
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Term
| The main features of programmed theory |
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Definition
Immune system compromise
Neurological degeneration
Hormonal theory of ageing
The genetic clock |
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Term
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Definition
| The Hayflick limit is the number of times a normal human cell population will divide until cell division stops. |
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Term
| What are the branches of regenerative medicine? |
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Definition
• Organ transplantation
• (Stem) Cell therapy
• Tissue engineering |
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Term
| What are the branches of transplantation? |
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Definition
Human donor organs
Xenotransplantation
tissue engineering |
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Term
What are the applications of genome engineering
? |
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Definition
human health
agriculture
biotechnology
research tools |
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Term
| Which nucleases increase inducing double strand breaks? |
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Definition
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Term
| What are the main characteristics of Zinc finger (ZnF) nucleases? |
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Definition
Based on C2H2 zinc finger domains
Can be assembled into arrays that bind the desired target sequence
Each domain binds 3 base pairs along
the major groove
Two ZnF arrays are used to target a specific locus
Each is fused to a monomer of FokI
Targeting efficiency increased by up to 1000 fold!
Cleavage occurs only upon dimerization
of the FokI domains |
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Term
|
Definition
| Transcription activator-like effectors (TALEs) |
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Term
|
Definition
| Clustered regularly interspaced palindromic repeats |
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