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Hastings Lab Mid-Term Practical
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73
Veterinary Medicine
Undergraduate 1
02/24/2013

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Cards

Term
What is the difference between the blue striped and red striped microhematocrit tube?
Definition
Red stripe has sodium heparin, Blue stripe has none.
Term
List of three layers of a blood smear:
Definition
Thick layer,
monolayer,
Feathered edge.
Term
How can you tell here in the monolayer?
Definition
No more than 50% of the red blood cells touch,
Cells are not distorted,
cells are evenly and randomly distributed
Term
how can you distinguish a band neutrophil from a segmented one?
Definition
There may not be any indentations in the nucleus greater than one third the diameter of the cytoplasm.

Cytoplasm may have slight basophilia.
Term
What are segmented neutrophils?
Definition
Mature neutrophils.
Term
Characteristics of a segmented neutrophil:
Definition
Nucleus is lobed, elongated, and contains clumpy chromatin.
Colorless cytoplasm that contains granules- may be colorless to pale pink.
Term
Where are segs found?
Definition
In the peripheral circulation.
Term
What is a hypersegmented neutrophil?
Definition
And neutrophil who's nucleus has 5 lobes or more.
Term
When might you see hypersegmented neutrophils in increased numbers?
Definition
Aged cells,
Maybe an artifact from prolonged storage of blood,
May see with use of glucocorticoids which prolongs the half-life of circulating neutrophils.
Term
What is the significance of finding hypersegmented neutrophils?
Definition
None.
Term
Characteristics of an eosinophil:
Definition
Lobed nucleus,
colorless cytoplasm containing red round cytoplasmic granules, variable shape and size.
Term
Characteristics of a reactive lymphocytes:
Definition
Typically have dark blue cytoplasm.
May also have prominent perinuclear clear zone.
Term
When might you see an increased number of reactive lymphocytes?
Definition
Seen in animals that are antigenically stimulated such as post vaccination.
Term
What type of anomaly is it when all segs are bands?
Definition
Pelger-Huet
Term
What diseases it when you see a lot of lymphocytes in one field?
Definition
Lymphocytic anemia
Term
What is it called when RBCs lack color?
What is this caused by?
Definition
Hypochromasia.

Caused by insufficient hemoglobin.
Term
What is it called when cells are clumped together?

What causes this?
Definition
Agglutination.

Antibodies coat the RBCs and makes them sticky.
Term
When would you see agglutination?
Definition
In immune mediated anemia.
Term
What is it called when cells are stacked like coins?

What animal is this common in?
Definition
Rouleaux

Common in horses.
Term
When might you see rouleaux in any species other than horse?
Definition
In inflammatory diseases,

If blood has sat too long.
Term
What type of cell is a target cell?
Definition
Leptocyte
Term
What species are leptocytes seen in?
Definition
Canine
Term
What does the appearance of leptocytes indicate?
Definition
Anemia,
liver disease,
and some inherited diseases.
Term
How are Leptocytes characterized?
Definition
Appearance of a mouth (stomatocytes),
Folded cells, or
Target cells.
Term
In what species are eosinophils common in numbers?
Definition
Horses
Term
What are characteristics of an eosinophil in a horse?
Definition
Bowling ball globular orange granules.
Term
Characteristics of an eosinophil in a dog?
Definition
Granules are pink and small.
Term
What are characteristics of degranulation of an eosinophil?
Definition
Granules are clear and the cytoplasm is red. The exact opposite of what it should be.
Term
What species of dog would you most commonly seen degranulation of an eosinophil?
Definition
Greyhounds
Term
Characteristics of a basophil?
Definition
Have blue lavender granules.

Are not as big and globular as eosinophils.
Term
What are the characteristics of an eosinophil of a cat?
Definition
Granules are rod shaped and orange pink in color.
Term
Hypersegmented neutrophils are commonly seen in what breed?
Definition
Poodle
Term
characteristics of a Monocyte:
Definition
Variably shaped nucleus and is less dense than a lymphocyte.

Abundant blue cytoplasm.

Vacuoles in cytoplasm common but not always present.
Term
What is Anaplasma Phagocytophilium?
Definition
A vector born zoonotic disease that causes lameness and is confused with Lyme disease.
Term
What is it called when you see a lack of platelets?
Definition
Thrombocytopenia
Term
What is it called when you have erythrocytes with decreased staining intensity?
Definition
Hypochromasia
Term
How can you differentiate between a hypochromatic RBC and a punch out cell?
Definition
If it were a punched out red blood cell you would only see a few, if it were hypochromasia, all the cells would look like that.
Term
When u see RBCs with no central pallor and are stained darkly and are smaller than normal RBCs. What are these called?
Definition
Spherocytes
Term
Variation in cell color?
Definition
Polychromasia
Term
Variation in size of RBCs.
Definition
Anisocytosis
Term
What are reticulocytes?
Definition
Immature erythrocytes that contains organelles that are lost as the cell matures.
Term
I know what kind of staining might you see organelles in reticulocytes?
Definition
NMB


NOT diff quik!
Term
What is Mycoplasma haemofelis?
Definition
A bacterial parasite of feline RBCs that cause hemolytic anemia.
Term
What do you get when u have an increased number of polychromatic RBCs?
Definition
Responsive anemia.
Term
Characteristics of mycoplasma haemofelis:
Definition
Chains of basophilic structures around the edges of the cells.
Term
What is the problem with automated blood cell counters and nRBCs?
Definition
They are counted as WBCs.
Term
What is nRBCs a response to?
Definition
Regenerative anemia if lots are seen.
Term
Who is the host for Cytauxzooin felis?
Definition
Bobcat
Term
What is Cytauxzooin?
Definition
A parasite that causes fatal disease in domestic cats. Seen as inclusion shaped like ringlets or safety pins.
Term
Another name for Acanthocyte?
Definition
Spur cell
Term
Characteristics of an Acanthocyte?
Definition
Uneven projections with dull points
Term
Another name for Echinocytes?
Definition
Burr cells
Term
Characteristics of Echinocytes?
Definition
Even needle like projections on entire edge of RBC.
Term
What causes Echinocytes?
Definition
Caused from slow drying of slide.
Term
Where do you commonly see platelet clumping?
Definition
Cats
Term
Who do you commonly see giant platelets in?
Definition
Cats
Term
Why does platelet clumping mean?
Definition
The number of platelets are adequate.
Term
Characteristics of Karatocytes?
Definition
RBC has a big clear hole on the edge. Looks like a blister.
Term
Another name for Karatocytes?
Definition
Blister cell
Term
Heinz bodies are commonly seen in what species?
Definition
Cats
Term
Characteristics of Heinz Bodies?
Definition
If stained with diff quik have a clear bleb on the outside of cell. If stained with NMB will have a blue bleb on the outside of cell.
Term
What are Howell Jolly bodies?
Definition
Basophilic inclusions of young erythrocytes.
Term
When are Howell jolly bodies commonly seen?
Definition
Seen in most domestic species during a regenerative anemia.

Cats
Term
What is a Schistocyte?
Definition
RBC fragmented formed as a result of the intravascular trauma.
Term
When are Schistocytes seen?
Definition
Seen in cases of DIC
(disseminated intravascular coagulation)
Term
What is it called when you see very small, variably sized granules with in the RBC?
Definition
Basophilic stippling.
Term
When might you see basophilic stippling?
Definition
Can be seen in response to lead poisoning.
Term
Who is Babeseia Gibsoni seen in commonly?
Definition
In 50% of pit bulls.
Term
Characteristics of Babaseia Gibsoni?
Definition
Pear shaped inclusion,
Often but not always occurs in pairs,
Smaller than Babaseia Canis
Term
What is Babenia Canis?
Definition
A syndrome of anemia, hemoglobinuria, and jaundice.
Term
Characteristics of Babesia Canis?
Definition
Pear shaped,
Basophilic in color.
Term
What is Anaplasma Platys?
Definition
A condition where platelets look like they have granules. It is often confused with a typical granulated platelet.
Term
Characteristics of Anaplasma platys?
Definition
Smaller then RBC,
Stained blue/purple,
Looks darker than regular platelet.
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