Squamous Epithelium

Basal Cells

Squamous Epithelium

Superficial - Pink

Intermediate - Blue

Endocervical Cells

Columnar shape, basal nuclei, granular cytoplasm

Normal endocervical cells

Honeycomb pattern

Squamous Metaplasia

Under rapid cell devision, cytoplasmic processes

Squamous Repair

Enlarged cell nuclei, prominent nucleoli

Typical repair

Classical pattern of cervical erosion

Atypical repair

Large nuclei, prominent nucleoli

Radiation changes

Polychromasia, vacuolisation, cell enlargement

High Grade Epithelial Abnormality

V. small, high n:c ratio, hyperchromatic (v. dark)

Low Grade HPV

Clearing aound nucleus, dark smudgy nuclei

Low Grade

Large cell, dark smudgy nucleus, low n:c ratio

Low Grade

Orange and smudgy nuclei

Low Grade

Genital wart, koilyocytes

High Grade CIN3

Fragile - large sheet, not much cytoplasm

CIN 3

Superficial cells with subpopulation of small dark cells

Adenocarcinoma In Situ

(top gland, bottom left gland normal)

Carcinoma

Bright orange staining, lots of variation, nucleoli, dirty background (blood + cell debris)

Endocervical adenocarcioma

Presence of nucleoli

Endocervical adenocarcinoma

Diathesis in background, large cytoplasmic vacuoles, prominent nucleoli

Endocervical adenocarcinoma

Vacuolated, delicate cytoplasm

Normal endometrial cells

Small dark clumps, cuboidal

Exodus of endometrial cells

Dark central core (epithelial type) and pale outside (stromal type)

Lower Uterine Segment

Look very similar CIN 3 but more uniform and generally form tubular structures

Endometrial Carcinoma

Cytoplasmic vacuole (top right)

Ovarian Adenocarcinoma

3D, hyperchromatic, prominent nucleoli, clean background

Smear obscured by cytolysis

Smear taken very late in menstrual cycle (drop in estrogen) --> cells broken down