Term
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Definition
| Fixes and sticks a dye in a cell. |
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Term
| Gram Stain chemistry for Gram + |
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Definition
- Fixed Smear: Colorless
- Crystal Violet: Purple
- Gram's iodine: purple
- Ethanol: purple
- Safranin: purple
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Term
| Gram Stain Chemistry for Gram - |
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Definition
- Fixed smear: colorless
- Crystal violet: purple
- gram's iodine: purple
- ethanol: colorless
- safranin: red/pink cells
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Term
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Definition
| dissolves lipopolysaccharide layer that is present in gram + cells |
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Term
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Definition
| Cells filled with crystal violet-iodine complex can't accept safranin, and remain purple |
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Term
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Definition
| Cells emptied of crystal violet- iodine complex able to accept safranin, and appear red/pinK |
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Term
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Definition
IMPORTANT: IF older than 24hrs , peptidoglycan may break down
This means gram + can look gram - |
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Term
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Definition
- Makes sure that cells will remain adhered to glass slide
- Cells are killed, preventing release of degrading enzymes,
- icreases rigidity of cellular structures
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Term
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Definition
Chemical groups whic possess visible color and either a positive or negative charge.
Basic; pos, attracted to neg charge components (NA)
Acidic: neg, attracted to pos charged like proteins. |
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Term
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Definition
| Cells unstained, background has color |
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Term
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Definition
| cells stained, back colorless |
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