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Microbiology Gram- Mac + Ox+ Bacilli
Pseudomonas, Burkholderia, Alcaligenes, Vibrio, Aeromonas, Plesiomonas
68
Microbiology
Graduate
11/25/2012

Additional Microbiology Flashcards

 


 

Cards

Term
What kind of infections are nonfermenters associated with?
Definition
Gastroenteritis
Term
Who is at risk for infections due to nonfermenters?
Definition
Immunocomprimised patients
Term
When is isolating B. cepacia from a respiratory specimen important?
Definition
When you patient has CF, because it can be life threatening
Term
What is the predominate pulmonary pathogen in CF patients?
Definition
P. aeruginosa
Term
B. cepacia
Definition
Non motile and oxidase positive
Term
Does susceptibility testing have to be done on P. aeruginosa?
Definition
Yes
Term
Mesophilic
Definition
grows best between 25-40°C
Term
Meliodosis
Definition
disease endemic to SE Asia
Term
Pyoverdin
Definition
water soluble fluorescent pigment
Term
cystic fibrosis
Definition
genetic syndrome characterized by chronic infection
Term
pyocyanin
Definition
blue green pigment
Term
B. bronchiseptica
Definition
normal respiratory flora, to differentiate it from others it is indole and urease positive and motile
Term
What medium is best used for Vibrio
Definition
TCBS
Term
What test will diffentiate Plesiomonas from Shigella?
Definition
Oxidase
Term
Does V. cholorae require salt for growth?
Definition
No
Term
Wound infections in saltwater fishermen
Definition
V. parahaemolyticus
Term
Halophilic
Definition
salt loving
Term
Pandemic
Definition
worldwide epidemic
Term
Cholera
Definition
toxin mediated diarrheal disease
Term
Dysentery
Definition
diarrhea producing blood and WBCs
Term
Cary-Blair
Definition
transport medium
Term
TCBS agar
Definition
selective agar for vibrio
Term
CIN agar
Definition
selective agar for yersinia
Term
Common characteristics of Vibrio
Definition
Gram negative bacilli, Grows on MacConkey, Glucose Fermenters, Oxidase Positive and Indole Positive
Term
Vibrio growth on Mac
Definition
NLF
Term
Yellow on TCBS
Definition
Sucrose positive, V. cholerae and V. alginolyticus
Term
Green on TCBS
Definition
Sucrose negative, v. parahemolyticus and v. vulnificus
Term
Biochemical Tests
Definition
indole postive, oxidase positive, NaCl positive
Term
Diseases of Vibrio cholerae
Definition
Cholera and Gastroenteritis, septicemia, and would infections
Term
Toxin associated with Vibrio Cholerae
Definition
subunit B to bind to receptor on cell membrane and subunit A to accumulate cAMP
Term
Environment of Vibrio parahemolyticus and Vibrio alginolyticus
Definition
Lives in brackish or salt water
Term
Vibrio parahemolyticus and Vibrio alginolyticus associated with
Definition
contaminated seafood
Term
Diseases of Vibrio parahemolyticus and Vibrio alginolyticus
Definition
Gastroenteritis (V. parahemolyticus), Wound infections, septicemia, ear infections
Term
diseaes due to Vibrio vulnificus
Definition
septicema
Term
Common characteristics of Aeromonas
Definition
positive for gelatin, negative for everything else, Oxidase, indole positive
Term
diseases associated with Aeromonas
Definition
Gastroenteritis, Wound infections, Septicemia
Term
disease of Aeromonas due to
Definition
water associated, contaminated food
Term
Gram stain of Aeromonas
Definition
straight rods
Term
Aeromonas on blood
Definition
large round colonies, beta hemolysis
Term
Aeromonas on MAC
Definition
can be LF or NLF
Term
Common Characteristics of Plesiomonas shigelloides
Definition
negative for gelatin, seen in freshwater
Term
disease of Plesiomonas shigelloides
Definition
gastroenteritis
Term
Gram stain of Plesiomonas shigelloides
Definition
gram negative short rods, chains and filamentous forms
Term
Plesiomonas shigelloides on blood
Definition
shiny, smooth, opaque, nonhemolytic
Term
Plesiomonas shigelloides on MAC
Definition
can be LF or NLF
Term
Common Characteristics of Pseudomonas
Definition
Gram negative rods, straight, skinny, Growth on Mac- NLF, glucose nonfermenter, Oxidase positive
Term
Pseudomonas Habitat
Definition
water, respiratory tract, not normal flora
Term
transmission of Pseudomonas
Definition
exposure, nosocomial
Term
Virulence factors of Pseudomonas Aeruginosa
Definition
exotoxin A
Term
diseases of Pseudomonas Aeruginosa
Definition
otitis media, folliculitis, osteomyelitis, septicemia, endocarditis, nosocomial infections, common in CF patients, respiratory tract infections
Term
Pseudomonas Aeruginosa on blood
Definition
flat spreading colonies with serrated edges and metallic sheen, often beta hemolytic and produces a water soluble green pigment, with a grape-like odor, mucoid colonies
Term
Identification of Pseudomonas Aeruginosa
Definition
Grows at 42° C, Positive for arginine, citrate and acetamide
Term
treatment for Pseudomonas Aeruginosa
Definition
antipseudomonal beta lactams, Aminoglycosides, quinolones
Term
Disease of Burkholderia cepacia
Definition
causes life-threatening disease in CF patients, UTI, RTI, sterile body site infections
Term
Cultivation of Burkholderia cepacia
Definition
PC agar and OFPBL agar
Term
Identification of Burkholderia cepacia
Definition
Lysine positive
Term
treatment of Burkholderia cepacia
Definition
no definitive treatment
Term
Common Characteristics of Alcaligenes and Bordetella
Definition
Gram negative rod, MacConkey +, Oxidase +, Glucose non-utilizers
Term
Habitat of Alcaligenes
Definition
environment, soil, water, nosocomial
Term
diseases of Alcaligenes
Definition
opportunistic, UTI, Bacteremia, pneumonia, wound infections
Term
gram stain of Alcaligenes
Definition
medium to long straight rods
Term
Colony morphology of Alcaligenes
Definition
on blood nonpigmented, on Mac NLF
Term
identification of Alcaligenes
Definition
obligate aerobe, nitrate positive, indole, Esculin, gelatin negative
Term
Habitat of Bordetella
Definition
normal respiratory flora
Term
diseases of Bordetella
Definition
opportunistic pneumonia, Bacteremia, UTI
Term
gram stain of Bordetella
Definition
variable coccobacillus to medium rods
Term
colony morphology of Bordetella
Definition
on blood small, on Mac NLF
Term
identification of Bordetella
Definition
obligate aerobe, indole, Urease positive, motile
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