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Gram - Biochemical Tests
North Seattle CC (Microbio)
17
Biology
Undergraduate 2
11/27/2018

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Cards

Term
Indole Test
Definition

Produces enzyme tryptophanase?

 

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Term
MR Test
Definition

Produces acid end products from glucose fermentation?

 

pH indicator = methyl red

 

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Term
VP Test
Definition

Produces alkaline and non-acid end products from glucose fermentation?

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Term
Citrate Test
Definition

Metabolize citrate (sole carbon source)?

 

pH indicator = brom thymol blue

 

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Term
Sugar Fermentation Broths
Definition

Can ferment glucose, lactose, or sucrose?

Acidic = yellow

Alkaline = hot pink

Gas in tube?

 

pH indicator = phenol red

 

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Term
Urea Test
Definition

Produces enzyme urease?

 

Positive = Pink

Negative = Yellow

pH indicator = phenol red

[image]

Term
Phenylalanine Slants
Definition

Presence of enzyme phenylalanine deaminase?

 

Positive = Slant turns green

 

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Term
Motility Deeps
Definition

Produces flagella?

 

Positive = fanning of growth

 

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Term
Catalase Test
Definition

Produces enzyme catalase?

 

Positive = produces bubbles after adding hydrogen peroxide

Term
Oxidase Test
Definition

Presence of enzyme cytochrome oxidase?

 

Positive = blue color

Negative = clear

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Term
MacConkey Agar
Definition

Selective = Gram - Rods, Differential = lactose fermenter/non-fermenter

 

Positive = Pink

Negative = Clear

 

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Term
XLD Plate
Definition

Selective = Gram - Rods, Differential = Salmonella from Shigella

 

Ferments sugar --> yellow on agar

Salmonella = black

Other colonies = yellow

 

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Term
HE Plate
Definition

Selective = Gram - Rods

Differential = ferments lactose and/or sucrose

 

Ferment = orange/yellow

Don't Ferment = blue/green

 

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Term
Alpha hemolysis
Definition
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Term
Beta hemolysis
Definition
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Term
Gamma hemolysis
Definition
[image]a
Term
EMB Plate
Definition
Selective: Gram - Rods
Differential: lactose fermentation?

+: Dark or metallic sheen
-: clear
[image]
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