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Glucose Fermenting, Oxidase Pos, Curved Gram Neg Bacilli
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26
Microbiology
Undergraduate 3
04/19/2015

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Term
Glucose Fermenting, Oxidase Pos, Curved Gram Neg Bacilli include
Definition
Vibrionaceae and Camplyobacter spp
Term
Vibrionaceae
Definition
Vibrio spp
Aeromonas spp
Plesiomonas spp
Causes gastroenteritis usually after eating improperly cooked seafood
Term
Vibrio spp.
Definition
Associated with water
Will grow in increased salt concentration (some spp are halophilic meaning they require salt)
Identified only by request. Specimen needs to be collected before administering antibiotic therapy and transported to the lab immediately because they are sensitive to drying and pH changes
Term
Vibrio spp and media
Definition
Not fastidious and will grow on BAP and MAC. Does not grow on XLD and HE. Selective media is TCBS
Term
Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS)
Definition
Contains thiosulfate, ferric ammonium citrate (as H2S indicator)
oxfall (bile salt/ inhibits gram positives)
Sucrose (carb source)
Bromthymol blue (pH indicator yellow is acid and blue-green is alkaline)
Term
Vibrio cholera
Definition
Comma-shaped
Causes severe watery bacteria by producing enterotoxin
Was originally called Kommabacillus
Transmitted from fresh and salt water exposure and food cooked in contaminated water
Term
Pathophysiology of cholera infection
Definition
Organism produces enterotoxin which disrupts the normal function of intestinal mucosa and the toxin attaches to epithelial cells to activate CAMP which prevents the reabsorption of Na+ that results in "rice water stools"
Term
Treatment for cholera
Definition
Fluid replacement and tetracycline
Term
V. cholera identification
Definition
Yellow colonies and sucrose pos on TCBS. Non-halophilicm string test and shooting star motility
Term
Alkaline Peptone Water
Definition
Used as transport media for vibrio cholera. Decreased pH suppress the growth of other bacteria.
Term
String Test
Definition
Used for identifying vibrio cholera. Suspend the organism in 1 drop of 0.5% sodium desoxycholate (Bile solubility reagent) cholera will be positive and form a viscous string
Term
Serotyping of vibrio cholera
Definition
Based on the O somatic antigen
2 serogroups: 01 and non 01
01= intestinal disease
non 01= extra-intestinal infections
Term
Serogroup 01 of vibrio cholera can be divided into 3 groups that include? It can also be divided into 2 biotypes that include?
Definition
Inaba
Ogawa
Hikojima
2 biogroups: Classical and El Tor
El Tor is the most severe
Term
Vibrio parahemolyticus
Definition
Endemic in Japan and Chesapeake Bay area
ASsociated with oysters, clams and shrimp (can get it from eating improperly cooked seafood)
Causes gastroenteritis by exotoxin
Term
Is Vibrio parahemolyticus halophilic?
Definition
Yes
Term
Vibrio vulnificus
Definition
Causes wound infections due to exposure of contaminated water and can also cause septicemia
Halophilic
Term
Vibrio mimicus
Definition
Causes wound infections associated with water exposure
Halophilic
Term
Vibrio aginolyticus
Definition
Causes superficial wound infections along with ear and eye infections. Associated with salt water and swimming. Halophilic
Term
Aeromonas spp
Definition
Found in water and transmitted through contact with contaminated water or improperly cooked seafood. Commonly isolated from drains, traps and is a source of nosocomial infections. Also commonly causes infections in cold-blooded animals
Term
Pathogenesis of Aeromonas spp
Definition
Casue cellulitis, wound infections, gastroenteritism septicemia (especially in patients with liver disease
Term
Plesiomonas shigelloides
Definition
Found in fresh water and soil.
Infects cold-blooded animals. Causes gastroenteritis via enteropathogenic enterotoxin and is transmitted through contaminated seafood (shellfish) or water
Term
Campylobacter and Helicobacter spp
Definition
Curved, gram neg bacilli that are microaerophilic (need Co2)
Term
Campylobacter jejuni
Definition
Found in poultry, cattle and pigs. Causes up to 1/3 of all cases of diarrhea. (bloody diarrhea with neutrophils) Invades submucosa and is not toxin mediated.
Term
Campylobacter jejuni transmission
Definition
Partially or undercooked poultry, unpasteurized raw milk or contaminated water
Term
Helicobacter pylori
Definition
Associated with peptic ulcers and stomach cancer
Grows on Skirrow's media
Term
Skirrow's media
Definition
Used for Helicobacter pylori.
Horse blood/ Polymyxin and trimethoprim
Incubate microaerophilically at 35 degrees Celsius for 3-5 days
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