Term
What is the definition of a gene? |
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Definition
a segment of DNA that is involved in producing a polypeptide chain; it can include regions preceding and following the coding DNA as well as introns between the exons; it is considered a unit of heredity |
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Four classes of biological molecules |
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Definition
Lipids, Carbohydrates, Proteins, Nucleic Acid |
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fatty acids, phospholipids, steroid hormones, cholesterol |
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components of membranes, can diffuse through membranes |
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steroid hormone function? |
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Definition
bind to receptors to make cell specific functions |
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Simple sugars, oligosaccharides, polysaccharides |
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structural/storage materials such as starch, cellulose, peptidoglycan, glycogen, chitin |
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Eukaryotes vs. Prokaryotes |
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Definition
membrane bound organelles & no membrane bound organelles DNA enclosed in nucleus & DNA loosely packed by histone-liked proteins attached to inside of cell membrane |
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Definition
phospholipid/protein bi-layer |
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Term
What is cytoplasm and what does cytoplasm contain? |
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Definition
colloid of protein and water, Contains the other organelles and membrane systems such as endoplasmic reticulum and the ribosomes, site of protein synthesis. |
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Term
How do Ribosomes, endoplasmic reticulum, and Golgi work together to create and distribute proteins? |
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Definition
After synthesis on ribosomes, proteins acquire sugar tags in ER and are sorted and shunted after being prepared in Golgi. |
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Term
Mitochondria characteristics? |
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Definition
resemble Gram negative bacteria (2 membranes) in many ways, are self replicating and have own DNA, Site of aerobic respiration, ATP synthesis , DNA is circular like bacterial; bacterial-type ribosomes |
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Definition
carry out photosynthesis; have own DNA |
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Definition
centrioles are structures that occur in pairs and are made of microtubules, Microtubules are made of protein, Centrioles help assemble the spindle to which chromosomes attach in mitosis |
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Term
structure and function of cytoskeleton? |
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Definition
is made of microtubules and microfilaments, Provides cell shape, structure and locomotion |
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Definition
No organelles, little or no cytoskeleton, cell wall, and no nucleus |
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Scientist that first described nucleus? |
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Definition
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Scientist that discovered nucleic acid? |
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Definition
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Definition
threadlike structures in nucleus consists of proteins and DNA |
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Term
Why did Levene think that proteins were hereditary material? |
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Definition
Proteins were made of 20 different kinds of amino acids and DNA was only made of 4 nucleic acids |
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Term
Describe the experiment Griffith used to show inheritance? |
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Definition
Used streptococcus(smooth and rough) Smooth caused death in mice, heat treated smooth did not, but smooth heat treated and rough together did, conclusion the smooth gave DNA to rough |
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Term
Describe Avery, McLeod, and McCarty's experiment? |
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Definition
They used protease, RNAse, and DNAse on bacteria, no mutation when DNAse was used hence DNA is genetic |
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Term
Describe Hershey-Chase experiment? |
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Definition
used phage because it is 50/50 protein/DNA, isolated protein and DNA and used radioactivity to show infection |
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radioactive sulfer labeled protein |
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Definition
radioactive phosphorus labeled DNA |
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built double helix model with sugar phosphate chains |
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DNA asorbs light best at? |
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Definition
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Definition
thymine & cytosine, adenine & guanine |
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DNA chains are held together by? |
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Definition
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how many hydrogen bonds do A-T have? |
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Definition
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how many hydrogen bonds do G-C have? |
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Definition
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Definition
10 bp per turn 3.4 nm per turn Right-handed helix Central axis |
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what is semi-conservative? |
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Definition
each time DNA replicates the new double stranded DNA has one new and one old strand |
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decsribe meselen and stahl experiment? |
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Definition
grew DNA in two different nitrogen and came out with semiconservative DNA |
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Definition
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Term
how long does it take E. Coli to replicate? |
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Definition
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how fast do eukaryotes replicate? |
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Definition
10-100 base pairs per second |
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how fast do bacteria replicate? |
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who discovered DNA pol I? |
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Definition
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Definition
cleaves out Okazaki fragments and is the most abundant of the 3 |
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Definition
main DNA replicating enzyme |
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Definition
A chemical reaction in which molecules are combined to make a products |
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Definition
a natural biological process in which a DNA molecule is copied in a cell |
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Term
what does DNA polymerase need? |
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Definition
template of DNA, primer, and substrate |
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Term
what is a template of DNA? |
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Definition
Enzymes copy a single strand of DNA Can’t work without something to copy from |
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