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Genetics
Exam 1
50
Biology
Undergraduate 4
09/17/2009

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Term
What is the definition of a gene?
Definition
a segment of DNA that is involved in producing a polypeptide chain; it can include regions preceding and following the coding DNA as well as introns between the exons; it is considered a unit of heredity
Term
Four classes of biological molecules
Definition
Lipids, Carbohydrates, Proteins, Nucleic Acid
Term
DNA codes for?
Definition
proteins
Term
Examples of Lipids?
Definition
fatty acids, phospholipids, steroid hormones, cholesterol
Term
Hydrophobic?
Definition
components of membranes, can diffuse through membranes
Term
steroid hormone function?
Definition
bind to receptors to make cell specific functions
Term
Carbohydrates?
Definition
Simple sugars, oligosaccharides, polysaccharides
Term
Polysaccharides?
Definition
structural/storage materials such as starch, cellulose, peptidoglycan, glycogen, chitin
Term
Eukaryotes vs. Prokaryotes
Definition
membrane bound organelles & no membrane bound organelles DNA enclosed in nucleus & DNA loosely packed by histone-liked proteins attached to inside of cell membrane
Term
Describe cell membrane?
Definition
phospholipid/protein bi-layer
Term
What is cytoplasm and what does cytoplasm contain?
Definition
colloid of protein and water,
Contains the other organelles and membrane systems such as endoplasmic reticulum and the ribosomes, site of protein synthesis.
Term
How do Ribosomes, endoplasmic reticulum, and Golgi work together to create and distribute proteins?
Definition
After synthesis on ribosomes, proteins acquire sugar tags in ER and are sorted and shunted after being prepared in Golgi.
Term
Mitochondria characteristics?
Definition
resemble Gram negative bacteria (2 membranes) in many ways, are self replicating and have own DNA, Site of aerobic respiration, ATP synthesis
, DNA is circular like bacterial; bacterial-type ribosomes
Term
Chloroplasts?
Definition
carry out photosynthesis; have own DNA
Term
Describe centrioles?
Definition
centrioles are structures that occur in pairs and are made of microtubules, Microtubules are made of protein, Centrioles help assemble the spindle to which chromosomes attach in mitosis
Term
structure and function of cytoskeleton?
Definition
is made of microtubules and microfilaments, Provides cell shape, structure and locomotion
Term
describe prokaryotes?
Definition
No organelles, little or no cytoskeleton, cell wall, and no nucleus
Term
Scientist that first described nucleus?
Definition
Robert Brown
Term
Scientist that discovered nucleic acid?
Definition
Friedrich Miescher
Term
chromosomes?
Definition
threadlike structures in nucleus consists of proteins and DNA
Term
Why did Levene think that proteins were hereditary material?
Definition
Proteins were made of 20 different kinds of amino acids and DNA was only made of 4 nucleic acids
Term
Describe the experiment Griffith used to show inheritance?
Definition
Used streptococcus(smooth and rough) Smooth caused death in mice, heat treated smooth did not, but smooth heat treated and rough together did, conclusion the smooth gave DNA to rough
Term
Describe Avery, McLeod, and McCarty's experiment?
Definition
They used protease, RNAse, and DNAse on bacteria, no mutation when DNAse was used hence DNA is genetic
Term
Describe Hershey-Chase experiment?
Definition
used phage because it is 50/50 protein/DNA, isolated protein and DNA and used radioactivity to show infection
Term
what is s-35?
Definition
radioactive sulfer labeled protein
Term
what is p-32?
Definition
radioactive phosphorus labeled DNA
Term
Chargoff's foundings?
Definition
A=T, G=C
Term
Watson and Crick?
Definition
built double helix model with sugar phosphate chains
Term
DNA asorbs light best at?
Definition
260nm
Term
virus genetic molecules?
Definition
RNA
Term
pyrimidines and purines?
Definition
thymine & cytosine, adenine & guanine
Term
DNA chains are held together by?
Definition
hydrogen bonds
Term
how many hydrogen bonds do A-T have?
Definition
2
Term
how many hydrogen bonds do G-C have?
Definition
3
Term
Dimensions of DNA?
Definition
10 bp per turn
3.4 nm per turn
Right-handed helix
Central axis
Term
what is Z-DNA?
Definition
left-handed DNA helix
Term
what is semi-conservative?
Definition
each time DNA replicates the new double stranded DNA has one new and one old strand
Term
decsribe meselen and stahl experiment?
Definition
grew DNA in two different nitrogen and came out with semiconservative DNA
Term
Normal nitrogen isotope?
Definition
N-14
Term
how long does it take E. Coli to replicate?
Definition
30 minutes
Term
how fast do eukaryotes replicate?
Definition
10-100 base pairs per second
Term
how fast do bacteria replicate?
Definition
1500 base pairs per sec
Term
who discovered DNA pol I?
Definition
Kornberg
Term
What does pol I do?
Definition
cleaves out Okazaki fragments and is the most abundant of the 3
Term
what does pol II do?
Definition
repair DNA damage
Term
what is pol III?
Definition
main DNA replicating enzyme
Term
synthesis reaction?
Definition
A chemical reaction in which molecules are combined to make a products
Term
DNA replication?
Definition
a natural biological process in which a DNA molecule is copied in a cell
Term
what does DNA polymerase need?
Definition
template of DNA, primer, and substrate
Term
what is a template of DNA?
Definition
Enzymes copy a single strand of DNA
Can’t work without something to copy from
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