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Definition
| discrete whole number of traits with as opposed to those without. Represented by 2 or more distinct phenotypes within a species that is controlled by a single gene. (Mendelian traits-prescene of widows peak, freckles, and hair on arms.) |
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| some sort of varying curve of traits is formed. Represented by a range of phenotypes within a species that is controlled by multiple genes. (Multifactorial traits- determined by genes and environment-examples include height and weight.) |
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| determines how close your observed values are to expected ratio |
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| n-1 where n is the number of classes |
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| Probability- Chi square context |
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Definition
| deviation from predicted numbers at least as large as that observed in experiment will occur by chance. P value of >.05 means less than 5% chance of obtaining same results. <.05 are considered significant findings. |
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| DNA stain—used to visualize DNA by naked eye(in some cases) |
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| - embryo (sprouting) section of the wheat kernel, the remainder being endosperm (storage) |
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| generated by making dilutions from a known sample to create a range of concentrations (X-axis). Observations are Y-axis. |
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| measures amount of light that is absorbed by and transmitted through a sample. In spectroscopy absorbance A is defined as- Ax= log10 (Io/I) |
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| Absorbance of 260- (A260) |
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Definition
| DNA is measured at this absorbance. |
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| is directly related to amount of DNA in a sample |
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| protein in DNA sample can be measured by determining the optical density of a sample at A280. |
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Definition
| ratio of DNA protein in a sample |
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| DNA from wheat germ experiment |
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Definition
| .3 g of wheat germ and add 5 ml of detergent (opens up membranes) or SDS (sodium delete sulfate), .3g of meat tenderizer (enzyme called protease), .75ml of sodium bicarbonate (ph adjuster), 3 ml. of ice cold ethanol (perctipate DNA into layers), 200ul of DI water, 600ul of 70% ice cold ethanol. |
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