Term
| genes responsible for replication in prokaryotes: dnaA, dnaB, dnaC, and dnaG: which is which? |
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Definition
dnaA: Initiator proteins
dnaB: DNA helicase
dnaC: ladder proteins that load helicase
dnaG: primase |
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Term
| DNA melting is synonymous with: |
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Definition
| Denaturing, separation of DNA strands |
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Term
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Definition
| Single-strand binding; keeps DNA from annealing during replication |
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Term
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Definition
| pol III does "main addition." pol I excises RNA primers and replaces with analogous DNA |
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Term
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Definition
| gene in E.Coli where replication originates |
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Term
| Eukaryotic DNA polymerases: α,ε,δ |
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Definition
α: functions in priming, synthesizing an RNA primer
ε: loading strand complex
δ: lagging strand polymerase |
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Term
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Definition
| They bind to the 3' overhang via telomerase (check 3' vs 5' by drawing) |
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Term
| Initiation of transcription in bacteria: |
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Definition
| RNA pol holoenzyme (w/σ factor) recognizes -35 promoter, untwists DNA at -10 region; σ dissociates after ~8-9 nucleotides downstream |
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Term
| Transcription termination in bacteria: |
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Definition
ρ-independent: hairpin loop structure
ρ-dependent: C-rich and G-poor w/ no hairpin |
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Term
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Definition
70kDa: housekeeping (rpoD)
32kDa: heat stress (rpoH)
60kDa: N starvation (rpoN) |
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Term
| Eukaryotic RNA polymerases: |
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Definition
polI: rRNA
polII: mRNA, some snRNAs
polIII: most snRNAs, tRNAs |
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Term
| Eukaryotic transcription core promoter: |
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Definition
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Term
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Definition
| regulate transcription of eukaryotic genes |
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Term
| Initiation of eukaryotic transcription: |
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Definition
| GTF's bind first and recruit RNA polymerase |
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Term
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Definition
| TFIID binds to TATA. TFIIA and TFIIB bind to TATA-TFIID complex. RNA polII with TFIIF binds to pre-existing complex, then TFIIE and TFIIH bind and transcription begins |
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Term
| polycistronic vs monocistronic: |
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Definition
| Polycistronic mRNA's encode multiple proteins, monocistronic proteins encode just one |
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Term
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Definition
| Adds 7-methylguanosine (unique 5'-5' phosphodiester bond makes nuclease activity difficult) |
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Term
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Definition
| U1 binds to 5' splice junction |
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