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| A technique for testing the genotype of an embryo or fetus in utero with minimal risk to the mother or the child |
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BAC- bacterial artificial chromosome (vector) |
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Definition
| an F plasmid engineered to act as cloning vector that carry large inserts |
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| A pattern of dark spots in a developed photographic film or emulsion in the technique of autoradiography. |
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cDNA (copy DNA is from RNA) |
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| A synthetic DNA trancribed from a specific RNA through the action of the enzyme reverse transcriptase. |
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| Chorionic villus sampling (CVS |
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Definition
| A placental sampling procedure for obtaining fetal tissue for chromosome and DNA analysis to assist in parental dignosis of genetic disorders. |
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Cosmids as vectors- hybrids of lambda DNA |
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| a process results in a clone of identical cells, each containing the recombinant DNA molecule. |
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| an important enzyme in DNA replication and repair that seals the DNA backbone by catalyzing the formation of phosphodiester bonds. |
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| both strands have the same sequence ( nucleotide) but in antiparrellel orientation. |
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| an enzyme that can catalyze the synthesis of new DNA strands from a DNA template; several such enzymes exsist. |
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| a method of molecular separation in which DNA,RNA, or proteins are separated in a gel matrix according to molecular size with the use of an electrical filed to draw the molecule through the gel in a predetermined direction |
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| a library encompassing of the entire genome |
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Definition
1. to form a hybrid by cross
2. to ammeal complementary nucleic strands from different source |
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Lambda DNA (phage vector) |
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Definition
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Definition
| the transfere of electrophoretically separated RNA molecule from a gel onto the absorbent sheet, which is then immersed in a labeled probe that will bind to the to the RNA of interest |
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| vectors deliver DNA in a similar system but can accept inserts ranging from 80-100Kb |
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| A DNA sequence 180-degrees rotational symmetry |
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| an in vitro for amplifying a specific DNA segment that uses two primers that hybridize to oppsite ends of the segment in oppsite polarity and, over successive cycles, prime exponential replication of the segment only. |
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| carry genes for drug resistance |
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Definition
| the most used extensively used method for detecting specific macromolecules: a mixture of macromolecules ( DNA, RNA,or protein) is exposed to molecule-the probe-that will bind only with the sought-after macromolecule |
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Definition
| an RNA or DNA oligonucleotide that can serve as a template for DNA polymerase when annealed to alonger DNA molecule |
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| a novel DNA sequence formed by the combination of two nonhomologous DNA molecules. |
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| an endonuclease that will recognize specific target nucleotide sequence in DNA and break the DNA chain at those points; a variety of these enzymes are known, and they are extensively used in genetic engineering. |
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| a DNA fragment resulting from cutting DNA with a restriction enzyme |
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| specific DNA target sequence where DNA sequence is cut |
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| a method of DNA sequencing, it uses dideoxynucleotide triphosphate mixed with standard nucleotide triphosphate to produce a laddar of DNA strands whose synthesis is blocked at different lengths. |
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Definition
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Definition
| the transfere of electrophoretically separated fragments of DNA from a gel to an absorbent sheet such as paper; this sheet is then immersed in a solution containing a labeled probe that will bind to fragment of interest. |
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Definition
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| in cloning , the plasmid or phage chromosome used to carry the cloned DNA segement |
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| membrane carrying an imprint of proteins separated by electrophoresis. can be probed with labeled antibody to detect a specific protein. |
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YAC- yeast artificial chromosome (vector) |
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Definition
| a cloning-vector system in saccharomyces cerevisiae employing yeast centromere and replication sequences. |
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