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Genetics(a)
test1b
35
Biology
Undergraduate 4
09/18/2009

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Term
steps for Karyotype analysis?
Definition
chromosomes are spread, stained and viewed.
Term
Giemsa stain?
Definition
used in G banding
Term
Adult karyote methods?
Definition
use white blood cells, Treat with phytohemagglutinin, add colcemid, and squash cells (chromosomes spill out), stain them, and take pictures
Term
annealing?
Definition
renature protein or DNA
Term
FISH?
Definition
Fluorescent in situ hybridization
Term
Reasons for cell division?
Definition
Asexual reproduction of unicellular organisms
Development and growth of multicellular organisms
Wound healing
Maintenance and replacement
Term
what is mitosis?
Definition
nuclear division, an orderly division of genetic material between 2 daughter cells
Term
what is cytokinesis?
Definition
occurs near the end of mitosis, cytoplasm divided up as one cell becomes two
Term
a time of cell growth and general functioning.
Definition
G1
Term
all the DNA in the cell is doubled to prepare for division
Definition
S phase
Term
cell prepares for division.
Definition
G2
Term
interphase?
Definition
G1, S, and G2 phase
Term
cells not dividing; may never divide again, or may re-enter cycle when needed
Definition
G0
Term
actual dividing up of the copied chromosomes and distribution to daughter cells
Definition
mitosis
Term
cell cycle time?
Definition
G1-10hrs, S-9hrs, G2-4hrs, and mitosis-1hr
Term
cell cycle checkpoints?
Definition
G1/S checkpoint: is DNA in good condition? Has cell grown sufficiently in size, G2/M checkpoint: is DNA synthesis completed? Is DNA in good condition? M checkpoint: are spindle fibers formed? Are chromosomes attached to the spindle?
Term
proteins that phosphorylate other molecules
Adding a PO4 turns molecules “on” or “off”
Definition
kinases
Term
proteins that come and go with the cell cycle?
Definition
cyclins
Term
spindle forms, nucleolus disappears, nuclear membrane dissolves, chromosomes begin condensing.
Definition
prophase
Term
what is the kinetechore?
Definition
where spindle is
attached and pulls the chromosomes
Term
what happens during the S phase of the cell cycle?
Definition
each
chromosome is duplicated; these are not homologous chromosomes, but are
exact duplicates = sister chromatids
Term
where are sister chromatids attached?
Definition
centromeres
Term
chromosomes completely condense, line up along equator; sister chromatids visible, attached to spindle at centromeres
Definition
metaphase
Term
chromosomes are lined up along cell equator
Definition
prometaphase
Term
sister chromatids lined up along equator separate from each other, moving to opposite poles of the cell.
Definition
anaphase
Term
spindle disappears, nuclear membrane reforms, and chromosomes de-condense
Definition
telophase
Term
mitosis vs. meiosis?
Definition
diploid(2n), haploid(4n)
Term
a special nuclear division process for producing haploid gametes
Definition
meiosis
Term
Sister chromatids condense, become visible. Nuclear membrane dissolves; spindle forms
Definition
prophase 1
Term
stages of prophase I?
Definition
Leptonema: threads appear, homology search begins.
Zygonema: thickening continues, pairing occurs (synapsis), “bivalents” visible.
Pachynema: more thickening, “tetrads” visible, crossing over starts; sister chromatids are visible.
Diplonema: chiasmata are visible as pairs of sister chromatids begin to separate.
Diakinesis: chromosomes repel each other, chiasmata move towards ends of chromatids.
Term
what is synapsis?
Definition
when homologous chromosomes are paired up and at first stick to each other along their entire length.
Term
pieces that break and swap places in DNA?
Definition
crossing over
Term
Paired homologues line up along cell equator.
Definition
meiosis I
Term
NOT sister chromatids. Reduction division
Definition
anaphase I
Term
sister chromatids
separate, leaving
only 1 of each
type of
chromosome in
the cell
Definition
anaphase II
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