Term
| How do you make create a heat-fixed bacterial smear? |
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Definition
1. With inoculating loop, place a small drop of water on a clean glass slide.
2. With sterilized (flamed) loop, remove a small amount of the bacterial colony from the culture and mix thoroughly with the water on the slide.
3. Spread the mixture creating two regions: a dense cellular populated region and a less dense region (as seen below).
[image]
4. Let the slide air dry at room temperature. DO NOT HEAT.
5. Fix the air dried smear by quickly passing it three times through a flame until the slide is very warm to the touch. |
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Term
| Why do you not want to heat a bacterial smear while drying? |
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Definition
| Heating when liquid only boils bacteria, destroying their structure. |
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Term
| What power do you first use to examine a bacterial smear and find the stained cells to get them into focus? |
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Definition
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Term
| What do you need to place on your slide before observing a bacterial smear at 100x? |
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Definition
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Term
| Streptococcus psneumoniae are grouped as _____. |
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Definition
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Term
| Streptococcus pyogenes are grouped as _____ and are gram _____. |
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Definition
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Term
| Micrococcus sp. are grouped as _____ or _____, packets of four cells. |
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Definition
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Term
| E. coli are grouped as _____ and are gram _____. |
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Definition
| single organisms, negative |
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Term
| Who first developed gram staining? When was it developed? |
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Definition
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Term
| What does gram staining do? |
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Definition
| Separates virtually all bacteria into two groups. |
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Term
| What differential stain is the most common staining technique in all of microbiology? |
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Definition
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Term
| Upon completion of the gram staining procedure, bacteria that appear pink are said to be gram _____ and bacteria that appear purple are gram _____. |
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Definition
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Term
| What are the steps of gram staining? |
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Definition
1. crystal violet (primary stain)
2. iodine (mordant)
3. acetone (decolorizer)
4. red dye/safranin (counterstain) |
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Term
| After the addition of crystal violet and iodine, both gram positive and gram negative cells appear _____. |
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Definition
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Term
| After the addition of acetone in the gram-staining process, gram positive cells appear _____ and gram negative cells appear _____. |
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Definition
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