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Gen Lab Exam 2
Definitions
51
Biology
Undergraduate 2
03/24/2008

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Term
Mitosis
Definition
cell division producing 2 new cells with equal genetic material identical to the original cell
Term
Meiosis
Definition
Process by which the chromosomes are separated during the formation of gametes and their number reduced from diploid to haploid
Term
Chromosome
Definition

prokaryotes: intact DNA molecule containing the genome

Eukaryotes: DNA molecule complexed with RNA and proteins to form a threadlike structure containing genetic info arrangedi n a linear fashion 

Term
Chromatid
Definition
one of the longitudinal subunits of a replicated chromosome joined to its sister chromatid at the centromer
Term
Dyad
Definition
Product of a tetrad separtation at anaphase 1; consistes of 2 sister chromatids joined at the centromere
Term
Tetrad
Definition
4 chromatids that make up paired homologues in prophase 1 of meiosis
Term
Mitosis Cell Cycle
Definition

Interphase (G1, S, G2)

Prophase

Metaphase

Anaphase

Telophase (Cytokinesis) 

Term
Meiosis Cell Cycle
Definition

Prophase 1 ( Leptonema, Zygonema, Pachynema w/crossing over, Diakinesis)

Metaphase 1

Anaphase 1

Telophase 1

then

PMAT 2 

Term
N
Definition

refers to the number of chromosomes

 

1N=haploid

 

2N-diploid 

Term
C
Definition

refers to the amount of DNA per chromosome pair

 

before synthesis=2C

after=4C

 

after meiosis 1 and 2= 1C 

Term
What are the four different phase of the cell cycle?
Definition

G1, S, G2, Mitosis

Term
N refers to the ___ and C refers to ___.
Definition

number of chromosomes

amount of DNA per chromosome pair 

Term
Meiosis occurs in ___ cells only.
Definition
sex/gametes
Term
Ther term for division of cytoplasm is __.
Definition
cytokinesis
Term
Crossover occurs during the __ stage of meiosis 1.
Definition
Prophase 1, (pachynema)
Term
In drosophila if the C number is 8 before S phase and it will be _ after S phase?
Definition
16
Term
__ pigments of drosophila don't separate in the chromatography paper.
Definition
Brown
Term
Define Rf value
Definition

= distance solute travels

dist. solvent travels

 

Term

Chromatography is the separation of solutes base on

a. charge

b. adsportion

c. absorption 

Definition
b. adsorption
Term
First Division Segregation
Definition
when no crossing over occurs, the alleles separate in the first meiotic division
Term
Second division segregation
Definition
when crossing over occurs, alleles separate in the 2nd meiotic division
Term
chromatography:
Definition
separation of substances on the basis of their size and their affinity for the matrix used
Term
matrix:
Definition
solid material through which the mixture of the substances will travel
Term
Hemoglobin has __ polypeptide chains; there are 2 __ abd 2 __ strands.
Definition

four

alpha

beta 

Term
Each heme group has a __ ring structure with __ atom at its center.
Definition

porphyrin (N)

iron (Fe) 

Term
Sickle cell HB is caused by the substitution of the glutamic acid for _ at position 6 of the polypeptide
Definition
valine
Term
__ is the predominant Hb in fetus
Definition
HbF
Term

Cellulose acetate electrophoreitc separation depends on

a. charge and size of the molecule

b. adsorption

c. pH 

Definition
a. charge and size of the molecule
Term

Linked gens are part of the same chromosome

T/F? 

Definition
True
Term
What is a test cross?
Definition
between an unknown genotype against a known homozygous recessive genotype
Term
PCR stands for __?
Definition
Polymerase Chain Reaction
Term
Name the enzyme that is involved in the polymerization of DNA
Definition
DNA Polymerase
Term

The primer is a short sequene of about 10 nucleotides long and is made up of RNA.

T/F 

Definition
True
Term
In the agarose gel electophoreis the migration of DNA is base on __ and __
Definition

Charge

size 

 

Term
Longer DNA fragments migrate __  in the agarose gel compared to shorter fragments and are seen at the top of the gel.
Definition
shorter distances
Term
Give one example of PCR.
Definition

Genetic testing, ex with flies

analyzing fly DNA 

Term
What is genetic drift?
Definition
an atypical sampling of a population which forms a new isolated population
Term
Hardy Weinberg law holds good when the population is _ and when _ are absent.
Definition

large

mutation, migration, selection, genetic drift, meiotic drive

 

(mutation/disruption) 

Term
community of sexually interbreeding or potentially interbreeding individuals.
Definition
population
Term
consists of all the genes in all their allelic forms in reproductive gametes of a population
Definition
gene pool
Term
the percentage of alleles of a given type in a population
Definition
gene frequency
Term
heritable changes in the DNA or chromosome structure resulting in the production of a different allele
Definition
Mutation
Term
wide variety of mechanisms responsible for modifying reproductive success of a genotype
Definition
selection
Term
movement of members of one population to another sexually compatible population
Definition
Migration
Term
Gene Frequency formula
Definition

p+q=1

 

p: frequency of dominant

q: frequency of recessive

 

obtain frequency= sample #/ total # 

Term
Genotype frequency formula
Definition

p2+2pq+q2=1

 

p: frequency of dominant

q: frequency of recessive

 

obtain frequency= sample #/ total #

 

Term
Genetic Drift
Definition
random change in gene frequency due to chance
Term
occurs when a population is initially established by a small number of individuals
Definition
Founder effect
Term
occurs when a population is drastically reduced in size and then refounded
Definition
Bottleneck effect
Term
Product of several genes, each which has a small yet equal effect on the overal phenotype
Definition
Polygenetic Phenotype
Term
Polygenetic traits affected by outside factors are called _
Definition
multifactorial traits
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