Term
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Definition
SS agar Test
Contains Brilliant Green and Bile Salt - Slective against G+
Lactose Fermenters - Magenta
Non Fermenters - Pale
http://rci.rutgers.edu/~microlab/applied%20micro/schedulelinks/salmonellashigella.htm |
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Definition
Triper Sugar Iron Agar
Glucose Fermentors - Red Slant, Yellow Butt
Lactose and/or Sucrose - All Yellow
H2S Production - Black |
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Term
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Definition
Lysine Decarboxylase Broth
Use: Diffentiation of Salmonella
Positive Result: Purple (lyssine Decaboxylase Present)
Negitive Resut: Yellow
Produces: Cadaverine |
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Term
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Definition
Brilliant Green Lactose Bile Test
Use: Colifrorm Detection
Selects: G+ bacteria that ferment lactose
Enezyme: Formate Haydrogenlyase -> Gas Present
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Term
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Definition
MacConkey Agar
Use: Isolation of Enterobacteriaceae
Positive: Magenta Color - Lactose fermentor - E Coli
Yelow: Enterics which do not ferment lactose |
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Term
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Definition
Phenol Red Broth + Dulcitol (PRD)
Use: Differentiaion of Salmonella
Fermentation of Dulcitol -> Yellow Color
Gas Formation + Yellow = Salmonella |
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Term
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Definition
Bismuth Sulfite Agar
- Isolalation of Salmonella
- Enzyme: Sulfite Reductase
- Brilliant Green - Slelective against G+
- Salmonela Produces H2S from sulfite, creates elemental bismuth to make a metalic sheen.
- Positive Reation: Brown and Sheen
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Term
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Definition
Eosin Methylene Blue Agar
- Coliform Detection
- Dark Colonies/Metalic -> Lactose Fermenters
- Pale/Light Colonies -> Non Lactose Fermenters
- Gram + -> Usualy do not Grow
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Term
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Definition
Simmons Citrate Agar
- Organisms who only consume citrate as a carbon source can grow
- Positive Result is Blue
- Salmonella can consume citrate, E Coli Cannot
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Term
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Definition
Mannitol Yolk Prlymyxin Agar - MYP
- Inhibits growth of G-
- Use: Selection of Bacillus Species
- Enzyme: Lecithinase ->Cloudiness around colony
- Manitol Fermentation - Bacillus -> Yellow Colonies
- Selection and Diffentiation
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