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mutant strains unable to synthesize certain essential molecules from minimal ingredients: nutritional mutants |
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Beadle and Tatum's hypothesis that each gene coded for one polypeptide NOT specifically an enzyme or a protein |
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making a mRNA copy of DNA |
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messenger RNA; single strand complementary copy of the DNA transcript |
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synthesis of a polypeptide on a ribosome |
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modification of the mRNA before it leaves the nucleus; intron excision, poly A cap, and 5' cap |
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initial (unprocessed) RNA transcript of DNA |
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3 nucleotide sequence of DNA |
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DNA strand of the double helix that is transcribed into mRNA |
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starting to read the mRNA genetic code from the correct starting point so the correct sequence of nucleotides can be turned into the correct amino acids |
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enzyme the pries the two stranded DNA apart and adds RNA nucleotides to form the mRNA |
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stretch of DNA transcribed into an RNA molecule |
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region of DNA were RNA polymerase attaches and initiates transcription |
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collection of proteins in eukaryotes that mediates the binding of RNA polymerase and the initiation of transcription |
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promoter DNA sequence in the initiation complex of transcription |
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RNA base sequence that functions as the termination signal to transcription |
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modified guanine nucleotide that is attached to the end of a mRNA molecule-protects the mRNA from degradation and helps it attach to a ribosome in the cytoplasm |
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30-200 adenine molecules added to the end of a mRNA to prevent degradation in the cytoplasm and help it attach to the ribosome |
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the removal of introns from a mRNA molecule |
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non-coding DNA sections; junk DNA |
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expressed DNA; codes for a polypeptide |
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proteins that remove introns from the mRNA |
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transfer RNA; carries the amino acids to the ribosome during polypeptide synthesis |
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nucleotide triplet on the tRNA that is complementary to the codon on the mRNA |
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relaxation of base pairing in the third nucleotide of a codon |
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aminoaceyl-tRNA synthetase |
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Definition
enzyme that assists in the joining of amino acids to tRNA molecules |
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ribosomal RNA; makes up the ribosmes; two subunits, the large and small |
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holds the tRNA molecule carrying the next amino acid to be added to the polypeptide chain |
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holds the tRNA carrying the growing polypeptide |
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discharges tRNA molecules from the ribosome |
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protein that targets each polypeptide to a specific location |
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changes in the genetic material of a cell |
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replacement of one nucleotide and its partner in the complementary strand with another pair of nucleotides |
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altered codons still code for an amino acid and makes sense....just not the correct sense |
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alterations that change an amino acid codon to a stop signal |
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occurs when the number of nucleotides inserted or deleted is not a multiple of 3 |
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physical and chemical agents that interact with DNA to cause mutations |
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test for the mutagenic activity of a chemical |
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