Term
| What is the purpose of viral transport media? |
|
Definition
| The purpose of viral transport media is to keep the human cell culture alive in order to support the virus and keep it alive for identification |
|
|
Term
| What is contained in the viral transport media? |
|
Definition
| The transport media contains proteins, sugars and salts to keep the cells alive. |
|
|
Term
| How does a shell vial differ from a cell culture used in diagnostic virology? |
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Definition
| The shell vial differs from the cell culture in that the shell vial is a mixture of the cell culture and immunofluorescence. The shell vial contains a layer of cell line at the bottom, and it allows for rapid ID via immunofluorescence by utilizing a high speed centrifuge to increase the rate of viral attachment. |
|
|
Term
| In latex agglutination testing, what coats the latex particles? |
|
Definition
| In latex agglutination an antibody or an antigen can coat the latex particle, it depends whether you are searching for an antibody or an antigen |
|
|
Term
| detection molecule in ELVIA |
|
Definition
|
|
Term
| detection molecule in EIA/ELISA |
|
Definition
|
|
Term
| detection molecule in IFA/DFA |
|
Definition
| monoclonal antibody with fluorescent molecule attached |
|
|
Term
| detection molecule in Chemilluminescence |
|
Definition
|
|
Term
| detection molecule in Amplification techniques |
|
Definition
| fluorescence, luminal, radioactive material, or chromagens |
|
|
Term
| 5. A patient has a positive HIV screen. The western blot confirmatory test was negative. What may have caused this discrepancy |
|
Definition
| The western blot could have been negative because the patient hasn’t seroconverted yet, or the western blot was searching for HIV 2 and you were testing with an HIV 1 sample |
|
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Term
| An HIV RNA PCR test is ordered for the patient. What additional information will the molecular test give the physician? |
|
Definition
| The PCR will give the physician information about viral load or the amount of virus present. |
|
|
Term
| What is Latex Agglutination |
|
Definition
| Qualitative and semiquantitative w/end point titres on Serum |
|
|
Term
| ASO – anti-streptolysin O latex agglutination for? |
|
Definition
|
|
Term
| Cryptococcal Antigen Latex Agglutination on which type of specimen? |
|
Definition
|
|
Term
| Monospot (EBV) Latex Agglutination for? |
|
Definition
|
|
Term
|
Definition
| Clumping of visual charcoal particles |
|
|
Term
| RPR – Rapid Plasma Reagin Ab test on and for? |
|
Definition
| Plasma or serum Screening test for syphilis |
|
|
Term
| VDRL – Venereal Disease Research Laboratory on? |
|
Definition
|
|
Term
|
Definition
| Ag-Ab chromogen reaction on nitrocellulose strip |
|
|
Term
|
Definition
|
|
Term
| Pneumococcal Ag test on which sample? |
|
Definition
|
|
Term
| Legionella Ag on which sample? |
|
Definition
|
|
Term
| Mycoplasma pneumoniae testing for what antibody in which specimen? |
|
Definition
|
|
Term
| Rapid Influenza A&B on which specimen? |
|
Definition
|
|
Term
| Rapid HIV (EIA) on which specimen? |
|
Definition
|
|
Term
|
Definition
| automated or manual with spec used for C. diff toxin |
|
|
Term
|
Definition
| Mumps, Measles, Rubella, VZV, EBV, CMV, Herpes, Toxoplasma (serum) |
|
|
Term
|
Definition
| Luminol when mixed with oxidizing agent |
|
|
Term
| As electrons return to their ground state... |
|
Definition
| excess energy is liberated as a photon of visible blue light |
|
|
Term
| chemilluminescence measured on |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
1. Hep BSAg
2. Hep C Ab
3. Hep A IgM
4. Hep BC IgM |
|
|
Term
| HBsAG -, anti-HBc -, anti-HBs - = |
|
Definition
| susceptible to hep B infection |
|
|
Term
| HBsAG -, anti-HBc +, anti-HBs + = |
|
Definition
| immune from past infection |
|
|
Term
| HBsAG -, anti-HBc -, anti-HBs + = |
|
Definition
| immune from hep B vaccination |
|
|
Term
| HBsAG +, anti-HBc +, IgM anti-HBc +, anti-HBs - = |
|
Definition
|
|
Term
| HBsAG +, anti-HBc +, IgM anti-HBc -, anti-HBs - = |
|
Definition
|
|
Term
| HBsAG -, anti-HBc +, anti-HBs + = |
|
Definition
| (1) recovering from acute (2) immune and anti-HBs not detectable (3) susceptible to false-positive anti-HBc or (4) chronically infected and HBsAG not detectable |
|
|
Term
| HIV Ab screen can be done via |
|
Definition
| ELISA, rapid EIA or Chemilluminescence |
|
|
Term
| HIV & West Nile Confirmatory Test |
|
Definition
|
|
Term
| Immunofluorescence can be |
|
Definition
|
|
Term
| FTA – Fluorescent Treponema Antibody confirms |
|
Definition
|
|
Term
| Respiratory Panel contains |
|
Definition
| Adenovirus, Influenza A&B, Para influenza 1,2 & 3, RSV |
|
|
Term
|
Definition
| unique physical characteristics that can be observed |
|
|
Term
|
Definition
|
|
Term
|
Definition
| indirect identification targeting unique antibodies |
|
|
Term
|
Definition
| unique nitrogenous base sequences |
|
|
Term
|
Definition
| Molecular Structure of Nucleic acids |
|
|
Term
|
Definition
| PCR (Polymerase Chain Reaction) |
|
|
Term
|
Definition
Extraction
Denaturation
Primer anneal
Extension
Detection of products |
|
|
Term
|
Definition
|
|
Term
| Real-time polymerase chain reaction |
|
Definition
| amplify and simultaneously quantify a targeted RNA or DNA sequence. The amplified DNA is detected as the reaction progresses in real time, a new approach compared to standard PCR, where the product of the reaction is detected at the end. |
|
|
Term
| Method for detection in Real Time-PCR |
|
Definition
| sequence-specific probes consisting of oligonucleotides labeled with a fluorescent reporter |
|
|
Term
| Abbreviations used for real-time PCR |
|
Definition
|
|
Term
| Real-time reverse-transcription PCR is denoted as |
|
Definition
| qRT-PCR, RRT-PCR,or RT-rt PCR |
|
|
Term
| acronym RT-PCR commonly denotes |
|
Definition
| reverse-transcription PCR and not real-time PCR |
|
|
Term
|
Definition
| Strand displacement amplification |
|
|
Term
|
Definition
| Nucleic acid sequence based amplification |
|
|
Term
|
Definition
| transcription mediated amplification |
|
|
Term
|
Definition
|
|
Term
|
Definition
| PCR detection of MRSA from nasal swabs |
|
|
Term
| Hybridization Used for speciation of AFB |
|
Definition
| genprobe or labeled probe |
|
|
Term
| Frederick Sanger – The Nobel Prize in Chemistry 1980 |
|
Definition
|
|
Term
|
Definition
3 billion base pairs in 23 chromosomes
Only 1.5% of the human genome codes for proteins
2003 |
|
|
Term
| Gene sequencing of Actinomycetes |
|
Definition
|
|
Term
| HCV genotype testing is used to determine |
|
Definition
| required length and potential response to interferon-based therapy |
|
|
Term
|
Definition
Chip w/oligonucleotide DNA probes
Intensity of hybridization is determine using imaging processing software |
|
|
Term
|
Definition
| complete viral particle comprised of genetic material & the capsid |
|
|
Term
|
Definition
|
|
Term
|
Definition
| enveloped, helical, icosohedral |
|
|
Term
|
Definition
| ssRNA, dsRNA, dsDNA or ssDNA |
|
|
Term
|
Definition
Viral RNA serves as mRNA
for translation |
|
|
Term
|
Definition
| Virus provides RNA polymerase to convert to positive sense RNA |
|
|
Term
|
Definition
|
|
Term
|
Definition
| stool CSF, NP or throat swab |
|
|
Term
| Respiratory panel done on |
|
Definition
| patient specimen or patient infected mixed cell line (r-mix) |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Respiratory Syncytial Virus |
|
|
Term
|
Definition
|
|
Term
|
Definition
| triggers a cell to become cancerous, HIV |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Urine, Plasma, CSF or Tissue |
|
|
Term
|
Definition
| Marburg Virus and Ebola Virus |
|
|
Term
|
Definition
| protein particles that can destroy nerve tissue |
|
|
Term
| Creutzfeldt-Jacobs Disease |
|
Definition
|
|
Term
| It is best to collect specimens for viral isolation during |
|
Definition
| first few days of illness, during the febrile, acute (IgM) period |
|
|
Term
| Blood for virus collection to |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| scanning electron microscope |
|
|
Term
|
Definition
| transmission electron microscopy |
|
|
Term
|
Definition
|
|
Term
|
Definition
| synica, inclusions, lysis, enlargement |
|
|
Term
|
Definition
Tagged MCAb + Virus infected cell
Respiratory panel, CMV, HSV, VZV |
|
|
Term
|
Definition
| Tagged Ab + MCAb + Virus infected cell |
|
|
Term
| Rapid cell culture w/fluorescent staining used on which vial with what virus |
|
Definition
|
|
Term
|
Definition
| enzyme linked virus inducible system |
|
|
Term
|
Definition
| Hamster Kidney Cells w/Beta galactosidease gene |
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|
