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Final Practical
Body Fluid Differential
32
Science
Graduate
04/07/2013

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Term
Perform differential exam on body fluid specimens
Definition
100 white cell count, reported as a percentage. The specimen is centrifudged by using the cytocentrifuge and then looked at using low power than high power with oil.
Term
Neutrophilic granulocytes
Definition
segs, bands, metas, and myelos, and pros all appear flattened or rounded, contain granules.
Term
Eosinophils/basophils
Definition
look for colored granules
Term
Lymphoyctes
Definition
larger with more cytoplasm, round nucleus and clear cytoplasm (no vacuoles or granules)
Term
Monocyte/Macrophages
Definition
large cells with convoluted nucleus, abundant cytoplasm and vacuoles, possible pseudopods
Term
Mesothelial cells
Definition
tissue lining cavities, large cells with abundant blue/blue-gray cytoplasm, eccentric, rounded nucleus with fine chromatin, possible multinucleation and vacuoles
Term
Disintegrated WBCs
Definition
cellular or nuclear membrane has disintegrated
Microorganisms: must be confirmed immediately with gram stain, note intracellular or extracellular
Term
Birefringence
Definition
ability to refract light rays using a polarizing microscope, the quartz compensator is added to ID crystal based on velocity of light rays passing through the crystal’s grain (axis)
Term
MSU
Definition
monosodium urate- intra or extracellular, long, thin, needle-like with pointed edges, single or in bundles, strongly birefringent, brilliant with polarized light, with quartz compensator negative birefringent, parallel to axis appear yellow (negative), indicates gout
Term
Calcium pyrophosphate
Definition
indicates psuedogout, appear as rhombic, square rods, compensation with polarized light gives positive birefringence, parallel to axis appears blue (positive
Term
Cholesterol
Definition
appear as notched, rhombic plates with negative birefringence (yellow), seen extracellularly,
Term
Segmented neutrophils seen in
Definition
pleural, pericardial, and peritoneal fluid
Term
lymphocytes seen in
Definition
all fluids
Term
monocytes seen in
Definition
all fluids, low in CSF
Term
Eosinophils/basophils seen in
Definition
pleural, pericardial and peritoneal fluid
Term
benign mesothelial cells seen in
Definition
plural, pericardial and peritoneal fluid
Term
benign tissue cells seen in
Definition
Joint fluid and CSF
Term
Choroid plexus and ependymal cells
Definition
benign tissue cells in CSF, cluster with cytoplasmic granules, irregular nuclei
Term
what color do microorganisms stain?
Definition
blue
Term
most frequent fungal organisms?
Definition
Crytococcus, histoplasma, candida albicans, and candida tropicalis
Term
What do neutrophils in CSF indicate?
Definition
bacterial meningitis
Term
What do lymphocytes in CSF indicate?
Definition
viral or fungal meningitis
Term
What do eosinophils in CSF indicate?
Definition
parasite, allergies or drug reaction
Term
Why is synovial fluid thick?
Definition
hyaluranic acid
Term
why is synovial fluid aspirated?
Definition
to distinguish between crystal-inducing disease and a septic process
Term
Most common crystals present in joint fluid
Definition
monosodium urate crystals, calcium pyrophosphate crystals and cholesterol crystals
Term
[image]
Definition
Benign Meso cells
Term
[image]
Definition
Choroid plexus
Term
[image]
Definition
Lymph
Term
[image]
Definition
Macrophage
Term
[image]
Definition
Monos
Term
[image]
Definition
MSU
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