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Perform differential exam on body fluid specimens |
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Definition
100 white cell count, reported as a percentage. The specimen is centrifudged by using the cytocentrifuge and then looked at using low power than high power with oil. |
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Neutrophilic granulocytes |
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segs, bands, metas, and myelos, and pros all appear flattened or rounded, contain granules. |
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look for colored granules |
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larger with more cytoplasm, round nucleus and clear cytoplasm (no vacuoles or granules) |
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large cells with convoluted nucleus, abundant cytoplasm and vacuoles, possible pseudopods |
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tissue lining cavities, large cells with abundant blue/blue-gray cytoplasm, eccentric, rounded nucleus with fine chromatin, possible multinucleation and vacuoles |
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cellular or nuclear membrane has disintegrated Microorganisms: must be confirmed immediately with gram stain, note intracellular or extracellular |
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ability to refract light rays using a polarizing microscope, the quartz compensator is added to ID crystal based on velocity of light rays passing through the crystal’s grain (axis) |
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monosodium urate- intra or extracellular, long, thin, needle-like with pointed edges, single or in bundles, strongly birefringent, brilliant with polarized light, with quartz compensator negative birefringent, parallel to axis appear yellow (negative), indicates gout |
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indicates psuedogout, appear as rhombic, square rods, compensation with polarized light gives positive birefringence, parallel to axis appears blue (positive |
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appear as notched, rhombic plates with negative birefringence (yellow), seen extracellularly, |
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Segmented neutrophils seen in |
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pleural, pericardial, and peritoneal fluid |
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Eosinophils/basophils seen in |
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pleural, pericardial and peritoneal fluid |
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benign mesothelial cells seen in |
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Definition
plural, pericardial and peritoneal fluid |
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benign tissue cells seen in |
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Definition
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Choroid plexus and ependymal cells |
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Definition
benign tissue cells in CSF, cluster with cytoplasmic granules, irregular nuclei |
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what color do microorganisms stain? |
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Definition
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most frequent fungal organisms? |
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Crytococcus, histoplasma, candida albicans, and candida tropicalis |
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What do neutrophils in CSF indicate? |
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What do lymphocytes in CSF indicate? |
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Definition
viral or fungal meningitis |
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What do eosinophils in CSF indicate? |
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Definition
parasite, allergies or drug reaction |
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Why is synovial fluid thick? |
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Definition
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why is synovial fluid aspirated? |
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Definition
to distinguish between crystal-inducing disease and a septic process |
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Most common crystals present in joint fluid |
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Definition
monosodium urate crystals, calcium pyrophosphate crystals and cholesterol crystals |
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