Term
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Definition
Culture media allows some organisms to grow, but inhibits the growth of other organsims are called______media. |
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Term
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Definition
| Culture media that supports the growth of many organisms, but allows observation of visible differences based on differing characteristics among the various organisms is called_______media. |
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Term
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Definition
| The inhibitor used in mannitol salt agar is___? |
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Term
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Definition
| MacConkey agar is selective for the growth of ________organisms. |
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Term
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Definition
Mannitol Salt agar is selective for the growth of _________organisms.
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Term
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Definition
| Eosin Methylene Blue (EMB) agar is selective for the growth of ________organisms? |
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Term
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Definition
| Sabourad Dextrose agar (SDA) is selective for the growth of _________organisms? |
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Term
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Definition
| The pH indicator used in mannitol salt agar is _______? |
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Term
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Definition
| Growth of pink or purple colonies on MacConkey agar is indicative of the fermentation of_________? |
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Term
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Definition
| Eosin Methylene Blue agar is differential based on the fermentation of____? |
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Term
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Definition
| Mannitol fermentation produces acid which lowers the pH and causes the phenol red indicator to turn_____? |
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Term
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Definition
| Growth of dark pink or purple colonies on Eosin Methylene Blue agar is indicative of the fermentation of_____? |
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Term
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Definition
| Lactose fermentation is indicated on Eosin Methylene Blue agar by the growth of _______colored colonies? |
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Term
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Definition
| In the absence of mannitol fermentation, the indicator remains what color? |
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Term
| pale pink, white, or colorless |
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Definition
| Non-lactose fermenters appear what color on both MacConkey and EMB agar? |
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Term
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Definition
Which of the following bacteria would grow in MacConkey agar?
E Coli
S auerus
S epidermidis
Streptococcus pneumoniae |
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Term
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Definition
Which of the following bacteria would grow in Mannitol Salt agar?
Streptococcus pneumoniae
S aureus
E coli
Pseudomonas aeruginosa
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Term
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Definition
Which of the following organisms would grow in EMB agar?
S auerus
Strep pneumo
Enterococcus fecalis
E coli |
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Term
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Definition
Which of the following organisms seen in lab fermented mannitol on the mannitol salt agar?
S auerus
Staph epidermidis
Strep pneumo
E coli
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Term
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Definition
Which of the following seen in lab fermented lactose in the Macconkey agar by turning the colonies pink?
Pseudomonas aeruginosa
Strep pneumo
Staph auerus
E coli |
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Term
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Definition
| Which of the following bacteria seen in lab fermented lactose on the EMB agar by turning the colonies dark purple? |
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Term
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Definition
| What enzymes turns hydrogen peroxide into oxygen and water? |
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Term
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Definition
| What enzyme turns the bacteria purple on the filter or white paper? |
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Term
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Definition
Which of the following organisms is catalase positive?
Strep pneumo
Entercoccus fecalis
E coli
Stap aureus
Pseudo aeruginosa |
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Term
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Definition
Which of the following bacteria is oxidase positive?
E coli
Pseudo aeruginosa
Staph auerus
Strep pneumo
Entercoccus fecalis |
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Term
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Definition
| What enzyme is used to differentiate between Staph and Strep? |
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Term
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Definition
| Which species of gram positive organisms is known to be catalase positive? |
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Term
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Definition
| Which species of gram positive organims is known to be catalase negative? |
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Term
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Definition
| Which species of gram positive bacteria has a morphology of cocci in clusters? |
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Term
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Definition
| Which species of gram + bacteria has a morphology of cocci in chains? |
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Term
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Definition
| Which strep which you identified in lab has a gram positive diplococci appearance and shows as alpha hemolysis on blood agar? |
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Term
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Definition
| Which streptococcus has a gram + cocci in chains appearance and shows as gamma hemolysis on blood agar? |
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Term
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Definition
| Which Staph species is the only species that is coagulase+? |
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Term
| Crystal Violet-Iodine-Alchohol decolorizer-Safranin |
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Definition
| What are the order of solvents used in gram staining? |
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Term
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Definition
Which of the following did you have to do first when you tried to identify your unknown organism?
Grow the organism on mannitol salt agar
Grow the organism on macconkey agar
Catalase test
Oxidase test
Gram stain |
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Term
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Definition
| After gram staining your bacteria, it shows a gram positive cocci and you are unclear whether it is Strep or Staph based on morphology alone, which test would you run to differentiate between the two? |
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Term
| Either macconkey agar or EMB agar |
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Definition
| After gram staining your bacteria it shows to have the morphology of a gram negative bacilli, and now you want to differentiate between whether it ferments lactose or not, which agar would you use? |
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Term
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Definition
| After gram staining your bacteria, it shows to have a gram+ cocci in cluster morphology, which agar would you use to differentiate between the species of this organism? |
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Term
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Definition
| Which organism did you identify in lab which had a gram- bacilli morphology, non lactose fermenter, and was oxidase positive? |
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Term
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Definition
| Which organism did you identify in lab which showed a gram- bacilli morphology, fermented lactose and was oxidase negative? |
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Term
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Definition
| Which organism did you identify in lab whic showed a gram- bacilli morphology, non lactose fermenter and was oxidase negative? |
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Term
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Definition
| Which organism did you identify in lab that showed a gram positive cocci in clusters, was catalse postive, and fermented mannitol? |
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Term
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Definition
| Which organism did you identify in lab that showed a gram positive cocci in chains morphology, was catalase- and showed gamma hemolysis on blood agar? |
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Term
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Definition
| What color does Pseudomonas aeruginosa turn on nutrient agar which you witnessed in lab? |
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Term
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Definition
| What agar was used to show hemolysis in the Streptococcus species in lab? |
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