Term
Who discovered reverse transcriptase?
A) Howard Temin and David Baltimore
B) James Watson and Francis Crick
C) Rosalind Franklin
D) Barbara McClintock |
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Definition
| B) By reverse transcribing mRNA into cDNA |
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Term
What is the purpose of fragmentation in microarray analysis?
A) To degrade DNA and RNA molecules
B) To amplify DNA and RNA molecules
C) To isolate specific gene sequences
D) To ensure uniform hybridization on the array |
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Definition
What is the purpose of fragmentation in microarray analysis?
A) To degrade DNA and RNA molecules
B) To amplify DNA and RNA molecules
C) To isolate specific gene sequences
D) To ensure uniform hybridization on the array |
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Term
Which of the following is an application of microarrays in gene regulation studies?
A) Identifying disease-causing alleles in humans
B) Analyzing cell-specific gene expression
C) Detecting chromosomal deletions and duplications
D) Quantifying DNA amplification during PCR |
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Definition
| B) Analyzing cell-specific gene expression |
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Term
In microarray analysis, what does it mean when a sample hybridizes with a probe?
A) The sample binds specifically to the probe sequence.
B) The sample undergoes PCR amplification.
C) The sample undergoes denaturation.
D) The sample is digested by restriction enzymes. |
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Definition
| A) The sample binds specifically to the probe sequence. |
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Term
What is the purpose of using fluorescent dyes in quantitative real-time PCR?
A) To visualize the DNA amplification process
B) To quantify the amount of DNA in the sample
C) To identify specific gene sequences
D) To determine the melting temperature of the DNA |
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Definition
| B) To quantify the amount of DNA in the sample |
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Term
Who discovered reverse transcriptase?
A) Howard Temin and David Baltimore
B) James Watson and Francis Crick
C) Rosalind Franklin
D) Barbara McClintock |
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Definition
| A) Howard Temin and David Baltimore |
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Term
What is the function of reverse transcriptase?
A) Copies DNA into RNA
B) Copies RNA into DNA
C) Initiates transcription in gene expression
D) Degrades RNA molecules |
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Definition
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Term
Which type of RNA is usually the most abundant in an active cell?
A) mRNA
B) tRNA
C) rRNA
D) Small nuclear RNA |
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Definition
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Term
How are mRNA molecules separated from other RNA types in the total RNA fraction?
A) By using affinity chromatography
B) By using alkaline treatment
C) By using synthetic oligo-dT
D) By using ribonucleases (RNAse) |
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Definition
| A) By using affinity chromatography |
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