Term
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Definition
| nutrient substrates that can be used for the growth of microorganisms in the lab |
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Term
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Definition
1.liquid medium or broth (grow large # for examination)
2.solid medium (inoculated on surface to see colony growth or in slant tubes to look at chem rxn)
3. semisolid media (jelly-like used for motility or gelatin-hydrolysis testing) |
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Term
| Necessary nutrients to support growth |
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Definition
1. water
2. nitrongen-containing compounds
3. energy source (carbs, peptides, proteins)
4. accessory growth factors (blood serum) |
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Term
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Definition
| multi-purpose media used for general cultivation of most bacteria |
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Term
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Definition
| used to increase the # of microorgs of interest to sufficient levels for analysis while suppressing the growth of others |
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Term
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Definition
| satisfies the min. nutritional requirements of microorgs to be analyzed (i.e 'wild' yeast) |
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Term
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Definition
-basis media used to separate homogeneous cultures of microorgs
-may support growth of many microorgs types but has been modified to include inhibitory agents which restrict growth of undesired microorgs |
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Term
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Definition
| certain microbes produce characteristic colonies that are easily recognized (i.e. hemolytic vs. non-hemolytic) |
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Term
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Definition
| -a complex polysaccharide composed of galactose and galacturonic acid |
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Term
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Definition
-inorganic chemical that can be used in the place of agar as a solidifying agent
-resistant to microbial hydrolysis |
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Term
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Definition
| the chemical used as the acid/base indicator in Triple Sugar Iron(TSI) agar |
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Term
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Definition
| the range that should be counted when counting plates to determine an aerobic plate count |
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Term
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Definition
| non-selective agar that will support growth of most aerobic heterotrophic bacteria and will serve as a positive control for the viability of the orgs assayed |
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Term
| TCBS (Thiosulfate-citrate-bile salts-sucrose) agar |
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Definition
-a combination selective and differential media
-bile salts prevent growth of other strains of bacteria
-for isolation of Vibrio |
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Term
| EMB (Eoisin-methlylene blue agar) |
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Definition
-differentiate b/w enteric lactose fermeters and non-fermenters
-identification E.coli (blue-black w/ metallic green sheen caused by acid produced)
-used to isolate fecal coliforms |
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Term
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Definition
-differentiate among diff groups of enterbateriaceae (ferment glucose thru production of acid)
-phenol red indicates production of acid by change of color from orange/red to yellow
-H2S is produced as black ppt. |
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Term
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Definition
-contains bromothymol blue that turns yellow w/ prod of acid
-salmonella will produce alkaline rxn from decarbozylation of lysine to cadaverine
-salmonella will produce purple top and yellow butt |
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