Term
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Definition
| Samples of an unkown bacterium are placed in a drop of saline on slides and are treated with a known antiserum (antibodies). |
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Term
| ELISA (Enzyme-Linked Immunosorbent Assay) |
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Definition
| Known antibodies are placed in a microplate, amd an unknown bacterium is added to each well. A reaction indicates a positive result. Used in HIV testing. |
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Term
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Definition
| Proteins from known bacterium or virus are extracted by electrophoresis and transferred to a filter by blotting. Patient's serum is washed over the filter, and if antibodies are present, they will combine with test pathogen proteins. Anti-human serum is washed over the filter, and a visible colored band will appear after addition of the enzyme's substrate. |
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Term
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Definition
| Strands of DNA from 2 organism (1 known and 1 unkown) are heated to separation of their respective hydrogen bonds, creating two single strands. The strands of both organisms are attached, and the more base pair matches, the greater the match of the organism. |
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Term
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Definition
| Moving innoculated fluid is moved through a small opening. Can detect difference in conductivity for presence of bacteria. Scattering of a laser passed through the fluid can also help determine cell size, shape, density, fluorescence, and surface. Used to detect Listeria in milk. |
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Term
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Definition
| DNA from unknown organism is isolated, separated into strands, and fluoresced. DNA is then inserted into a chip of known DNA, and is allowed to hybridize to matching strands. Chip is washed, and matching strands will show positives through the fluorescent dye used. |
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Term
| FISH (Fluorescent in situ Hybridization) |
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Definition
| uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence complementarity. |
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