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EXAM #3
metabolism
22
Microbiology
Undergraduate 2
05/06/2010

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Term
During induction/repressor control of transcription the repressor protein binds to the _______ inhibiting ______ from binding to the ______
Definition
operator, RNA polymerase, promoter
Term
During the electron transport chain of aerobic FADH yields _____ ATP and NADH yields ____ ATP
Definition
2,3
Term
Requires elongation factors
Definition
bringing tRNAs with amino acids linked to the 3' end into the ribosome
translocation
Term
Accumulation of which product causes a bacterium to switch from aerobic respiration to fermentation
Definition
NADH
Term
Hair pin loop in RNA stops what
Definition
transcription
Term
Unwinds DNA
Definition
helicase
Term
S yields H2S. What types of metabolism could the bacteria be doing

Now, if it also converts protein to CO2. What types of metabolisms could it be doing
Definition
anaerobic respiration or anaerobic chemolithotropy
anerobic respiration
Term
What metabolism can carry out nitrification
Definition
aerobic chemolithotropy
Term
During carbon cycle what can NOT be used to fix CO2
Definition
anaerobic respiration
Term
You do an assay to isolate a mutation in the pathway to produce tyrosine. What media will you use
Definition
media without tyrosine and without antibiotics
Term
What is the source of electrons for the 1st and 2nd chlorophylls in the light reaction of photosynthesis
Definition
H2O, the first chlorophyll
Term
During the dark reaction of photosynthesis NADPH
Definition
reduces CO2
Term
What is the first thing you should do when cleaning sewage
Definition
allow organic matter to settle out of solution
Term
Addition of a phosphate to an amino acid in the enzyme that converts glycine into serine decreases the enzyme's ability to bing to glycine in the active site is an example of
Definition
negative covalent regulation
Term
What is the function of the Shine Delgarno sequence
Definition
to align the mRNA with correct site on the ribosome to start translation
Term
T/F In a eucaryotic gene, exons are sequences that are removed by splicing
Definition
False
Term
What amino acid will be added to a tRNA with the anti-codon 3' GCG5'
Definition
Arg
Term
codon changed from 5' CCC 3' to 5' CCA3' is what mutation
Definition
silent
Term
The enzyme that adds amino acids to tRNAs is called
Definition
amino acyl tRNA synthetase
Term
A bacterial gene that produces the enzyme that will break down starch into glucose molecules is what type on induction/repressor
Definition
induction and inducer is starch
Term
During transcription if A is int he template DNA strand, RNA polymerase will add a ___ in the mRNA
Definition
U
Term
When doing an assay to detect mutagens which is true?
a. you will use sterile violet to transfer bacteria between different plates
b. you will add mutagen to all plates int he assay
c. you will isolate the colony that can not grow on media that is lacking an essential component
d. all are true
e. none are true
Definition
e. none are true
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