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First to observe microorganism coined term "cell" saw dead cell with simple lens |
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First glimpses of microbial world saw living cell called organisms "animalcules" |
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Father of modern microbiology disproved spontaneous generation with flasks containing broth |
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formed small pox vaccine observing milk maids used living virus for vaccine in family |
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founder of aseptic surgery |
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created "germ theory" bacteria can cause disease |
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first to create a synthetic drug for treatment of syphillis |
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What are the 3 main domains? |
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describe Bacteria (domain) |
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most common type human infection widely diverse prominent features: specific shapes rigid cell walls multiply by binary fission takes 20 minutes to replicate |
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describe Archaea (domain) |
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prokaryotes live in extreme environments include: methanogens extreme halophiles extreme thermophiles |
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describe Eukarya (domain) |
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all members are eukaryotic composed of single celled: algae fungi protozoa |
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Bacteria Archaea cell type that lacks a membrane bound nucleus |
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Fungi protozoa algae helminths Has membrane bound organelles but no peptidoglycan |
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science that studies organisms to order and arrange them 3 Areas Identification Classification (Bergey's manual) Nomenclature |
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No Cell wall Hydrocarbons linked to glycerol ribosomes 70S No membrane bound nucleus |
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Yes peptidoglycan cell wall Fatty acids linked to glycerol ribosomes 70S No membrane bound nucleus |
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No peptidoglycan cell wall fatty acids linked to glycerol ribosomes 80S Yes membrane bound nucleus |
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obligate intracellular agents consist of either RNA or DNA surrounded by protein coat |
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obligate intracellular agents consist of only RNA NO protein coat |
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abnormal form of cellular protein consists of only protein NO DNA or RNA |
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Name 4 classes of Macromolecules |
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Protein Carbohydrates Lipids Nucleic acids |
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subunits of carbohydrates |
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subunits of nucleic acids |
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primary secondary tertiary quaternary |
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methods used to identify prokaryotes |
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microscope morphology metabolic capabilities serology |
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Bacillus (rod) Coccus Spiral spirillum (curved more than once 2D) vibrio (curved once) spirochete (3D) |
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3 forms of movement thru cytoplasmic membrane |
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simple diffusion facilitated diffusion activated transportaion |
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describe simple diffusion |
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move from [high] to [low] no energy needed |
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describe facilitated diffusion |
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move from [high] to [low] need a channel or transporter |
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describe activated transportation |
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move from [low] to [high] need a helper/ pump or carrier protein need energy |
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thick peptidoglycan NO outer membrane NO LPS or Porin sensitive to PVK & lysosomes |
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thin peptidoglycan, more susceptible to mechanical breakage Outer membrane, barrier to certain antibody contains LPS & Porin |
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flagella pilus fimbrae nuceoid (not nucleus) ribosomes |
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3 layers of membrane of prokaryotic |
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outermost ~capsule cell wall intermost~cell membrane |
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attachment DNA transfer (conjugation) |
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attachment avoid phagocytosis |
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protection physical osmotic lysis |
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control movement in and out of cell |
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