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Exam III
photosynthesis, cell division
123
Biology
Undergraduate 1
11/08/2008

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Term
Basic formula for photosynthesis
Definition
CO2 + H2O + (light energy)--> C6H12O6 + O2
Term
Internal membrane of chloroplasts
Definition
Thylakoid membrane
Term
What composes and what shape is the thylakoid membrane?
Definition
phospholipids; flattened sacs
Term
Thylakoids stacked one on top of each other in a column
Definition
Grana (sing. granum)
Term
What is the photopigment inside of chloroplasts?
Definition
chlorophyll (and other accessory pigments
Term
Semi-solid liquid surrounding the thylakoid membrane
Definition
stroma
Term
The three stages of photosynthesis:
Definition
1. Capturing energy from sunlight
2. using that energy to make ATP, reduce NADP+ to NADPH
3. Using ATP and NADPH to power synthesis of organic molecules to form O2
Term
What forms photosystems?
Definition
photosynthetic pigments that are clustered together
Term
True or False: pigments and the photons they absorb do not correspond to wavelengths of light.
Definition
False
Term
Which stages of photosynthesis are light-dependant?
Definition
The first two:
capturing energy from LIGHT
Using the energy to make ATP and reduce NADP+ to NADPH
Term
What is another name for the third stage of photosynthesis?
Definition
Carbon fixation:
formation of organic molecules from CO2
Term
True or False: Carbon fixation needs light to occur.
Definition
False: it can happen with or without presence of light
Term
Where are the photopigments located?
Definition
embedded in a protein complex in the thylakoid membrane (on top)
Term
What is the location of photosynthesis?
Definition
Leaf chloroplasts
Term
Which stage of photosynthesis is the calvin cycle?
Definition
The third.
Term
What other organelle are chloroplasts similar to?
Definition
Mitochondria
Term
What is the structure of chloroplasts?
Definition
Dbl. membrane; both membranes have lipid bi-layers; own DNA
Term
Where does stage 1 and 2 of photosynthesis occur?
Definition
In the discs of thylakoid
Term
What is the basic structure of chloroplasts?
Definition
Chlorophyll molecules consist of a porphyrin head and a hydrocarbon tail
Term
What structure allows the chlorophyll molecule to be embedded in the thylakoid membrane?
Definition
Its hydrocarbon tail binds to the hydrophobic portions of proteins (w/in the thylakoid)
Term
Which part of the chlorophyll molecule "sticks out" of the thyalkoid membrane?
Definition
the porphyrin head
Term
Where does step 3 of photosynthesis take place?
Definition
In the stroma.
Term
Where does the calvin cycle take place?
Definition
Outside of the thylakoid membrane in the stroma.
Term
What is the photoelectric effect?
Definition
A beam of light is able to remove electrons from certain molecules, creating an electric current.
Term
True of false: visible light has more energy than non-visible light
Definition
False. Visible light has much less relative energy.
Term
True or False: chlorophyll a and b absorb the green in the visible light spectrum.
Definition
False, the pigments are green because "green" is reflected back.
Term
When looking at the structure of a pigment, which part absorbs the light?
Definition
The porphryin head
Term
What is a function of Carotenoids, what do they keep at bay?
Definition
Free-radicals, which are made during photosynthesis and cellular respiration
Term
T o F: Carotenoids absorb the wavelengths of light just the same as chlorophyll
Definition
False; they absorb the "left-over" wavelengths that are not absorbed by chlorophyll a and b
Term
How are pigment molecules characterized?
Definition
By their absorption spectrum
Term
What system captures and channels photons into the reaction center chlorophylls?
Definition
The antenna complex
Term
Photosystems are composed of:
Definition
pigments and associated proteins held within a protein matrix on the surface of the photosynthetic membrane.
Term
What is RuBP?
Definition
5 carbon sugar and a phosphate that bonds with CO2 to form PGA during calvin cycle
Term
What is PGA?
Definition
A 3 carbon molecule and a phosphate that is made from RuBP and CO2 during calvin cycle
Term
What is needed before the calvin cycle begins?
Definition
ATP needs to be produced and NADP+ needs to be reduced to form NADPH
Term
When is ATP first used during the calvin cycle?
Definition
To add an additional phophate group to PGA
Term
What is the actual final product of the calvin cycle?
Definition
G3P (sugar)
Term
How many CO2 molecules does it take to produce one molecule of the final product of the calvin cycle?
Definition
three molecules to produce one molecule of G3P
Term
How many ATP and NADPH molecules does the calvin cycle consume to produce each G3P?
Definition
9 ATP and 6 NADPH
Term
What is used from the calvin cycle to produce glucose?
Definition
2 molecules of G3P
Term
What stores the energy that chlorophyll originally captured from the sun?
Definition
Glucose
Term
Photosystem I and II are basically what?
Definition
Light absorbing molecules
Term
What happens when pigments are excited?
Definition
energized electrons are released
Term
What accumulates inside the thylakoid to synthesize ATP?
Definition
H+ creates a proton gradient
Term
What replaces the e- lost during photosystem II's trapped molecule?
Definition
The splitting of water replaces much needed electrons.
Term
The splitting of water also serves to:
Definition
create a proton gradient by donating H+
Term
What helps make oxygen during photosynthesis?
Definition
the splitting of water
Term
Photosystem I or Photosystem II is where NADP+ is reduced to form NADPH
Definition
Photosystem I
Term
What are the two important products of the light-dependent reactions?
Definition
ATP and NADPH
Term
What is the area within a leaf where there is a high concentration of cells rich in chloroplasts?
Definition
The mesophyll layer
Term
How does the antenna complex work?
Definition
When the proper wavelength of light is absorbed w/in a photosystem. The excitation energy is transferred from one molecule to another until it goes to the reaction center
Term
Within the photosystems, what are the two subdivisions which are part of the chemical process?
Definition
1. antenna complex
2. reaction center
Term
What is the primary job of the antenna complex?
Definition
It is involved in light harvesting
Term
What is the primary job of the reaction center?
Definition
This is where photochemcial reactions occur.
Term
Human cells have ____ chromosomes, consisting of ____ identical pairs.
Definition
46; 23
Term
T or F:
In every eukaryotic organism, there is the same amt. of chromosomes.
Definition
False, each organism has different amounts.
Term
What composes chromosomes?
Definition
Chromatin
Term
What composes chromatin?
Definition
DNA and protein
40% - 60%
Term
In the cell, how is the DNA shaped and why?
Definition
It is coiled to allow a larger amount in a small area.
Term
Why do the histone proteins have the ability to bind to DNA which has a negative charged phosphate group?
Definition
Although proteins are normally negatively charged, histone proteins are positively charged.
Term
What is the particular type of chromosomes that an organism possesses called?
Definition
karyotype
Term
When speaking about the number of chromosomes, what is (n) and what is (2n)?
Definition
(n) is haploid
(2n) is diploid
Term
In humans, what is the diploid and haploid number of chromosomes?
Definition
the diploid number is 46
haploid is 23
Term
What ploidy number reflects the equal genetic contribution of each parent to make the offspring?
Definition
Diploid number of chromosomes.
Term
What is the difference between a sister chromatid and a homologous chromosome?
Definition
Sister chromatids are two replicas of the same chromosome (1 mom & 1 pop) held together at cetromere whereas the homologous chromosomes are not held together just the same chromosome, one from mom, one from pop
Term
What is the name of the protein which holds the chromosomes together?
Definition
Cohesins
Term
If the pair of the same chromosomes are called homologous, what is an individual chromosome (i.e. one from mom and one from pop, but they are the same chromosome)
Definition
homologue
Term
After DNA replication occurs, and the homologous chromosomes are linked, what are each individual called?
Definition
chromatids
Term
What stage does the cell spend most of its time?
Definition
interphase; G1
Term
In which stage does the nuclear envelope dissolve, chromosomes condense and the spindle fibers form?
Definition
Prophase
Term
What are the protein strands that reach from one end of the cell to the other?
Definition
microtubles
Term
At what stage are the chromosomes first visible?
Definition
Prophase
Term
What stage of mitosis is DNA synthesized?
Definition
S phase or interphase
Term
What is the name of the area where all the chromosomes congregate towards?
Definition
The metaphase plate
Term
During what stage of mitosis does the nuclear envelope develop on the two daughter cells?
Definition
Telophase
Term
What is the stage called when the cell is between divisions?
Definition
Interphase
Term
T or F: Cancer cells tend to effect the amount of chromosomes in a cell.
Definition
True: cancer can effect both an over abundance of cell growth and the number of chromosomes produced during cell replication.
Term
What are the key molecules that control and coordinate cell division?
Definition
CdK and Cyclin
Term
If you were to say who was in charge in a cell, who would it be?
Definition
CdK and Cyclin
Term
What is the main function during G1 phase of mitosis?
Definition
Growth phase
Term
What stage of mitosis does the cell stay in the longest?
Definition
G1
Term
Describe the S phase of mitosis
Definition
Synthesis, where the cell is replicating its DNA
Term
When speaking of mature nerve cells, what phase of cell replication do they stay in?
Definition
G0-the resting phase
Term
When there is no growth of a cell, what is that phase referred to?
Definition
G 0(zero)
Term
What is the stage of mitosis where chromosomes align in the center?
Definition
Metaphase (they align at the metaphase plate)
Term
What is the name of the movement of chromosomes toward the center of the cell?
Definition
Congression
Term
What is the quickest phase of mitosis?
Definition
anaphase
Term
What stage of mitosis is when the cohesion proteins are being broken down?
Definition
Anaphase
Term
At what stage in mitosis does the nuclear envelope reform?
Definition
Telophase
Term
Where do the spindle fibers attach during division?
Definition
Kinetochore
Term
Name the place where the sister chromatids attach.
Definition
centromere
Term
Where is the kinetochore located?
Definition
On the outside, the opposte side of chromosome
Term
What stage do the chromosomes start condensing?
Definition
prophase
Term
What is another term for late interphase?
Definition
G2
Term
What is the structure of a centriole?
Definition
9 triplets of microtubles and one core, two are always at right angles of eachother
Term
During what phase of division does the cell actually separate?
Definition
Cytokenisis
Term
In preparation for cytokinesis, what do animal cells form?
Definition
They construct a belt of actin filaments that pinch off and form a cleavage furrow
Term
During cytokinesis, how are plant cells different from animal cells?
Definition
Plant cells form cell plates and animal cells form cleavage furrows
Term
Comparing the beginning and the ending of mitosis, how many chromosomes are there?
Definition
The same amt.
Term
T or F:
Mitosis is always occurring in cells.
Definition
False; there is a G0 phase of mitosis where the cell is resting
Term
Stage where daughter cells separate
Definition
Cytokinesis
Term
What is a growth factor?
Definition
Proteins that promote mitosis
Term
What do growth factors bind to?
Definition
Integral proteins within the plasma membrane
Term
Why do growth factors bind to integral proteins?
Definition
To bind to receptors which work with cell signaling
Term
When growth factors bind to receptors and growth of a cell is needed, what happens next?
Definition
proteins which are needed for DNA synthesis to occur are signaled to start producing.
Term
When referring to cell signaling, what are the "things" that are told to start the process?
Definition
Cyclins.
Term
What are the enzymes called that modify proteins by adding a phosphate group to them?
Definition
Cdks (Cyclin dependent kinases)
Term
What are CdKs?
Definition
Enzymes that modify cyclin by adding a phosphate group to them.
Term
What activate Cdks?
Definition
Cyclins
Term
In the cell cycle, where are the checkpoints located?
Definition
G1 checkpoint
G2 checkpoint
spindle assembly checkpoint
Term
The G1 checkpoint checks what?
Definition
The size of the cell, nutrients, growth factors, and if there is any damaged DNA
Term
During the G2 checkpoint, what is being checked?
Definition
Cell size and DNA replication
Term
During the spindle assembly checkpoint, what is being checked?
Definition
Chromosomal attachment to the spindles
Term
What are the three features that are unique to meiosis?
Definition
Synapsis, homologous recombination and reduction division
Term
What is the process of forming complexes of homologous chromosomes (after replication)?
Definition
Synapsis
Term
What is crossing over?
Definition
When homologous chromosomes exchange genetic material.
Term
At the end of meiosis, each cell contains _______ the original complement of chromosomes.
Definition
Half (haploid)
Term
T or F: The sister chromatids that are formed after Meiosis II are always identical.
Definition
False; because of crossing over, the genes are "mixed"
Term
Homologous chromosomes are chromosomes which consist of genetic material from both _________ and ________.
Definition
Mother and father
Term
What is it called when there are different forms of genes?
Definition
alleles
Term
During what phase of meiosis do non-sister chromatids pair?
Definition
Prophase of meiosis
Term
Why do the gametes produced by meiosis have different combination of alleles?
Definition
recombination and independent assortment
Term
The final amount of chromosomes for mitosis and meiosis are:
Definition
mitosis diploid (same as when started)
Meiosis haploid four cells with half of the amt. of chromosomes
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