Term
True or False:
Do NOT Refrigerate CSF |
|
Definition
|
|
Term
Match the timing of collection with the specimen:
Urine |
|
Definition
|
|
Term
Match the timing of collection with the specimen:
Blood Culture |
|
Definition
| Around the time of a temperature elevation (fever) |
|
|
Term
| In general, should you collect a specimen before or after the use of antibiotics? |
|
Definition
| Before the use of antibiotics |
|
|
Term
True or False:
The lab needs Sufficient quantity of specimen for all test requests |
|
Definition
|
|
Term
| Appropriate container for the specimen |
|
Definition
Must be sterile containers!
Depends on the type of specimen |
|
|
Term
| What is the purpose of transport media? |
|
Definition
Prevents sample from drying out
Provides nutrients for MO
Inhibits specific microbial growth |
|
|
Term
What are the following considered:
Stuarts, Cary-Blair, & Amies gel, viral |
|
Definition
|
|
Term
|
Definition
| Specimen should reach lab within 2 hours |
|
|
Term
| Other than CSF, how should you other Specimens be stored? |
|
Definition
| Refrigerate sputum, urine, stool, abscess, etc. |
|
|
Term
True or False:
If a specimen is received unlabeled, missing any vital information, or does not match the requisition information, the specimen will be thrown out and a new one must be collected! |
|
Definition
|
|
Term
| What information is needed on the specimen label? |
|
Definition
Patient name
Patient identification number
Source: VERY important
Date/time collected
Other information
-Doctor name
-Antibiotics |
|
|
Term
True or False:
Of the types of specimens we went through in class the following are typically gram stained:
Abscess/Wound
Sputum
Body Fluids
Urethra (GC)
Stool (fecal WBC count) |
|
Definition
|
|
Term
| Describe a Cytospin Gram Stain |
|
Definition
Concentrate specimen in a small area on the slide
Increase chances of finding MO |
|
|
Term
True or False:
You should Perform Cytospin on all body fluids |
|
Definition
|
|
Term
| Is a HIGH # of PMNs indicative of a good quality or poor quality specimen? |
|
Definition
|
|
Term
| Is a HIGH # of Squamous Epithelial Cells indicative of a good quality or poor quality specimen? |
|
Definition
|
|
Term
| What does a HIGH # of PMN's on a gram stain indicate? |
|
Definition
| bacterial infection or inflammation |
|
|
Term
| What does a HIGH # of Squamous Epithelial Cells on a gram stain indicate? |
|
Definition
| Contamination from skin surface |
|
|
Term
| What should you do if a sputum sample has too many Squamous Epithelial Cells on its gram stain? |
|
Definition
| Reject the sample and request a new sample |
|
|
Term
Is the following appropriate or inappropriate GS Test:
Throat for GC? |
|
Definition
|
|
Term
Is the following appropriate or inappropriate GS Test:
Rectal for GC? |
|
Definition
|
|
Term
Is the following appropriate or inappropriate GS Test:
Vaginal for GC? |
|
Definition
|
|
Term
Is the following appropriate or inappropriate GS Test:
Throat for GAS? |
|
Definition
|
|
Term
True or False:
Do NOT set up stool gram stain for enteric pathogens |
|
Definition
|
|
Term
True or False:
A stool gram stain is OKAY for staphylococcus infections |
|
Definition
|
|
Term
True or False:
A stool gram stain may detect Campylobacter |
|
Definition
|
|
Term
True or False:
A stool gram stain is OKAY for PMNs |
|
Definition
|
|
Term
| Upon review of a sputum Gram stain, the technician notes that the nuclei of all of the neutrophils present in the smear are staining dark blue. The best explanation for this finding is: |
|
Definition
| the slide was inadequately decolorized w/ acetone/alcohol |
|
|
Term
True or False:
Urine is not routinely Gram stained |
|
Definition
|
|
Term
A Gram stained smear of a sputum specimen revealed the following cells:
3+ Squamous Epithelial Cells
3+ GNR
What should the microbiologist do next? |
|
Definition
| Reject the specimen - it is mouth flora |
|
|
Term
| Is a stool GS acceptable for Salmonella/Shigella? |
|
Definition
|
|
Term
| Name a few of the normally sterile body fluids |
|
Definition
| CSF, pleural, pericardial, synovial |
|
|
Term
| How soon should you process the BF cultures? |
|
Definition
|
|
Term
| How soon should you read and result the gram stain of normally sterile body fluids? |
|
Definition
|
|
Term
| What does the Cytospin GS do? |
|
Definition
|
|
Term
| Where should you inoculate normally sterile body fluid onto? |
|
Definition
|
|
Term
| What does Acridine Orange stain? |
|
Definition
| nucleic acids of living or dead cells |
|
|
Term
| What is the process of the Acridine Orange Stain? |
|
Definition
Prepare smear & fix with methanol
Flood with stain, rinse
Examine with 40X objective |
|
|
Term
| With the Acridine Orange Stain what will fluoresce orange? |
|
Definition
|
|
Term
| With the Acridine Orange Stain what color will Bacteria and Yeast fluoresce? |
|
Definition
|
|
Term
True or False:
CSF should be clear |
|
Definition
|
|
Term
| If CSF is turbid what will that indicate? |
|
Definition
|
|
Term
|
Definition
|
|
Term
| At what incubation environment do you hold CSF specimens? |
|
Definition
|
|
Term
| CSF leak through the nasal passage due to: |
|
Definition
Skull fracture
Blunt trauma
Tumors |
|
|
Term
True or False:
Strep pneumoniae can invade the CNS and cause meningitis |
|
Definition
|
|
Term
| Specimen Collection for Abscess/Wound Culture |
|
Definition
Avoid surface contamination
Look for PMNs in gram stain |
|
|
Term
| Types of specimens for Abscess/Wound Culture? |
|
Definition
|
|
Term
| Which specimen is BEST for Abscess/Wound? |
|
Definition
|
|
Term
| What do you sub Abscess/Wound specimens to? |
|
Definition
|
|
Term
True or False:
You can add anaerobic media for Abscess/Wound cultures when requested |
|
Definition
|
|
Term
True or False:
Must chop or grind the tissues to release any embedded MOs |
|
Definition
|
|
Term
What are the following considered:
Staph aureus
GAS
Enterics
Anaerobes
PSAR
Enterococci |
|
Definition
| Common Wound/Tissue Pathogens |
|
|
Term
What are the following considered:
Haemophilus influenzae
Strep pneumoniae
Neisseria meningitidis
GBS
Listeria
Staphylococcus (CNS) |
|
Definition
|
|
Term
|
Definition
| inflammation of stomach and intestines |
|
|
Term
| What are the specimens generally used for stool cultures? |
|
Definition
|
|
Term
| What do you store Stool or Rectal Swabs in? |
|
Definition
Sterile fecal collection container
Stool transport media (Cary Blair) |
|
|
Term
What are the following considered:
Stool culture (Salmonella & Shigella)
Fecal Gram Stains
Occult blood
Clostridium difficile toxin
Campylobacter sp. |
|
Definition
|
|
Term
| What do you perform fecal gram stain for? |
|
Definition
Fecal Leukocyte Count
Campylobacter
Staphylococcus |
|
|
Term
What are the following considered:
Salmonella
Shigella
E. coli 0157:H7
Yersinia enterocolitica
Campylobacter |
|
Definition
|
|
Term
What are the following considered:
Vibrio species
Aeromonas
Plesiomonas
Clostridium difficile
Staphylococcus aureus |
|
Definition
|
|
Term
| Stool cultures are usually for isolating ______ & _______. |
|
Definition
|
|
Term
| What do you sub Stool cultures to? |
|
Definition
BAP
MAC/EMB
HE/SS/XLD
GN Broth |
|
|
Term
| What is the incubation environment for stool culture media? |
|
Definition
|
|
Term
| What is the incubation environment for Campy BAP? |
|
Definition
|
|
Term
| What is the incubation environment for CIN? |
|
Definition
| Room temp (24oC) for 48 hours |
|
|
Term
What do you use the following specific stool cultures to isolate/identify:
Campy BAP |
|
Definition
|
|
Term
What do you use the following specific stool cultures to isolate/identify:
CIN |
|
Definition
|
|
Term
What do you use the following specific stool cultures to isolate/identify:
TCBS |
|
Definition
|
|
Term
What do you use the following specific stool cultures to isolate/identify:
SMAC |
|
Definition
|
|
Term
What are the following considered:
Cervical swab, vaginal, urethral discharge, prostatic fluid |
|
Definition
| Genital Culture Specimens |
|
|
Term
| When you setup the gram stain on a genital culture what are you looking for? |
|
Definition
Clue cells
GNDC (male urethral specimen only)
Yeast |
|
|
Term
| What do you sub genital specimen to? |
|
Definition
|
|
Term
| What is the incubation environment for genital culture media? |
|
Definition
|
|
Term
| What is the supplemental media for Genital cultures? |
|
Definition
Todd Hewitt/Carrot Broth
HBT or V agar
MAC/EMB |
|
|
Term
What is the MO that is hoped to be identified with the following Genital media:
MAC/EMB |
|
Definition
|
|
Term
What is the MO that is hoped to be identified with the following Genital media:
HBT or V agar |
|
Definition
|
|
Term
What is the MO that is hoped to be identified with the following Genital media:
Todd Hewitt/Carrot Broth |
|
Definition
|
|
Term
| What are the MOST common Genital Pathogens? |
|
Definition
N. gonorrhoeae
G. vaginalis
Yeast |
|
|
Term
What are the following considered:
H. ducreyi
Chlamydia trachomatis
Ureaplasma
Treponema pallidum
Herpes simplex |
|
Definition
| Other Common Genital Pathogens that require special techniques |
|
|
Term
| What are the Normal Flora MOs for the Genital area? |
|
Definition
|
|
Term
| What is the specimen for a Pharyngitis Culture? |
|
Definition
|
|
Term
| What AGN are you trying to detect with a Pharyngitis Throat Swab? |
|
Definition
|
|
Term
What are the following considered:
Group A strep
Groups C & G strep
Arcanobacterium haemolyticum
Gonococci
Viruses |
|
Definition
|
|
Term
| What is the most appropriate primary media battery for a CSF? |
|
Definition
|
|
Term
| What is the most appropriate primary media battery for a wound source? |
|
Definition
|
|
Term
| What is the most appropriate primary media battery for a stool source where the doctor suspects E. coli 0157:H7? |
|
Definition
|
|
Term
| What are some common organisms that cause meningitis? |
|
Definition
| S. pneumoniae, Group B streptococcus, H. influenzae |
|
|
Term
| The patient has pharyngitis and doctor suspects Neisseria gonorrhoeae. When the technologist receives the throat culture she/he should |
|
Definition
| Set-up the culture on BAP & MTM |
|
|
Term
| What are considered locations for/of Upper Respiratory Tract Infections? |
|
Definition
Pharyngitis
Sinusitis
Otitis Media/Externa
Epiglotittis |
|
|
Term
| What is a Good Specimen for Sinusitis? |
|
Definition
|
|
Term
| How is a good specimen collected for Sinusitis? |
|
Definition
| Sinus cavity is punctured with a needle and a sample of the sinus contents are collected |
|
|
Term
| What is a Poor Specimen for Sinusitis? |
|
Definition
|
|
Term
| When you perform a GS for Sinusitis what do look for? |
|
Definition
|
|
Term
What are the following considered:
Strep pneumoniae
H. influenzae (not Type B)
Moraxella catarrhalis |
|
Definition
| Common Acute Sinusitis MOs |
|
|
Term
What are the following considered:
Anaerobes
Staph aureus
H. influenzae
M. catarrhalis |
|
Definition
|
|
Term
What are the following considered:
Pseudomonas
Anaerobes
Enterics |
|
Definition
|
|
Term
| Inoculate sinus specimen to: |
|
Definition
BAP
CHOC
Broth
Anaerobic media |
|
|
Term
| What is the incubation environment for a Sinus culture? |
|
Definition
|
|
Term
| What is a GOOD specimen for Acute Otitis Media? |
|
Definition
|
|
Term
| What is a POOR specimen for Acute Otitis Media? |
|
Definition
| Nasopharyngeal/throat swab |
|
|
Term
| When you perform a GS for Acute Otitis Media what are you looking for? |
|
Definition
|
|
Term
| How do you collect a GOOD specimen for Acute Otitis Media? |
|
Definition
| puncture the typmanic membrane and apsirate fluid |
|
|
Term
What are the following considered:
H. influenzae
Moraxella catarrhalis
Strep pneumoniae |
|
Definition
|
|
Term
| Inoculate tympanic fluid to: |
|
Definition
BAP
CHOC
Broth
Anaerobic media |
|
|
Term
| What is the incubation environment for Otitis Media Culture? |
|
Definition
|
|
Term
| Which specimen is associated with "Swimmer's Ear"? |
|
Definition
|
|
Term
| What is the specimen usually for Otitis Externa? |
|
Definition
|
|
Term
| What is the MOST Common MO for Otitis Externa? |
|
Definition
|
|
Term
| What do you inoculate the swab for Otitis Externa to? |
|
Definition
|
|
Term
| What is the incubation environment for Otitis Externa Culture? |
|
Definition
|
|
Term
| Are Anaerobic cultures appropriate for Otitis Externa? |
|
Definition
|
|
Term
| What does Epiglottitis usually affect? |
|
Definition
|
|
Term
| What is the specimen typically used for Epiglottitis? |
|
Definition
|
|
Term
| What are the MOST common MOs for Epiglottitis? |
|
Definition
H. influenzae type B
Strep pneumo |
|
|
Term
| What do you inoculate the Epiglottis Culture Swabs to? |
|
Definition
|
|
Term
| What is the incubation environment for an Epiglottis Culture? |
|
Definition
|
|
Term
| Name 3 Lower Respiratory Tract Infections |
|
Definition
Pneumonia
Bronchitis
Lung Abscess |
|
|
Term
True or False:
The LRT is usually a sterile environment, except in patients with chronic pulmonary disease or tracheal tubes. |
|
Definition
|
|
Term
| Is a Gram Stain routine on LRT Specimens? |
|
Definition
|
|
Term
|
Definition
Expectorated or Induced Sputum
Bronchoscopy specimen |
|
|
Term
| What are 3 types of Bronchoscopy specimens? |
|
Definition
Bronchoalveolar Lavage (BAL)
Bronchial washing/brush
Biopsy |
|
|
Term
True or False:
In order for the sputum to be cultured, a gram stain must indicate possible infection |
|
Definition
|
|
Term
What are the following considered:
Strep pneumoniae
Group B streptococci (neonates)
Staphylococcus aureus
H. influenzae
Enterics
Pseudomonas
Anaerobes |
|
Definition
| Common Lower Respiratory MOs |
|
|
Term
| What do you Inoculate respiratory specimens to? |
|
Definition
BAP, CHOC, & EMB/MAC
Anaerobic media (if appropriate)
Broth |
|
|
Term
| What is the incubation environment for Lower Respiratory Tract Culture |
|
Definition
|
|
Term
| Name 3-4 Special Culture Media for the Respiratory Tract |
|
Definition
|
|
Term
| What Special Culture Media do you use to identify/isolate Legionella? |
|
Definition
|
|
Term
| What Special Culture Media do you use to identify/isolate B. cepacia? |
|
Definition
|
|
Term
| What Special Culture Media do you use to identify/isolate B. pertussis? |
|
Definition
|
|
Term
| What are some causes for UTIs? |
|
Definition
sex
kidney stones
catheterization
Diabetes melitus |
|
|
Term
| What is the BEST specimen for a UTI? |
|
Definition
| 1st morning pee is the Best! |
|
|
Term
| What are 2 UNACCEPTABLE urine specimens? |
|
Definition
Catheter tip (Foley catheter)
Pooled Urine |
|
|
Term
| What is The MOST Common UTI MO? |
|
Definition
|
|
Term
What are the following considered:
Enterics
Enterococci
Pseudomonas aeruginosa
Staph saprophyticus
Candida species (yeast)
E. coli |
|
Definition
|
|
Term
True or False:
You perform a GS on a Urine Sample by request ONLY |
|
Definition
|
|
Term
| What do you use to perform a urine culture? |
|
Definition
|
|
Term
| When performing a urine culture do you streak for isolation or streak for growth? |
|
Definition
|
|
Term
| What do you Inoculate urine sample to? |
|
Definition
|
|
Term
| What is the incubation environment for a urine culture? |
|
Definition
|
|
Term
| When performing a colony count on a urine culture, 1 colony type is what? |
|
Definition
|
|
Term
| When performing a colony count on a urine culture, 3 or more colony types in equal numbers is what? |
|
Definition
|
|
Term
| Urine Colony Count - If using a 1 μL loop, multiply the number of colonies by what? |
|
Definition
|
|
Term
| Urine Colony Count - If using a 10 μL loop, multiply the number of colonies by what? |
|
Definition
|
|
Term
| After multiplying by 1000 for 1 μL or 100 for 10 μL for a Urine Colony Count then what do you multiply it by? |
|
Definition
|
|
Term
| If a urine colony count is 100,000 or more, what will you report it as? |
|
Definition
|
|
Term
| When processing throat swabs for a GAS culture, the medium of choice is: |
|
Definition
|
|
Term
| Would you use BCYE with Legionella species? |
|
Definition
|
|
Term
| What primary media battery would you use for Sputum? |
|
Definition
|
|
Term
| Which of the following is the most appropriate method for collecting a urine specimen from a patient with an indwelling catheter? |
|
Definition
| Aspirate urine aseptically from the catheter tubing |
|
|
Term
| The lab receives a sputum specimen for routine culture. The direct Gram stain shows a predominance of pleomorphic GNB. The next day there is no growth on the BAP, but growth on the CHOC agar. What potential pathogen do you suspect? |
|
Definition
|
|
Term
| What is the incubation environment for a urine culture? |
|
Definition
|
|
Term
| When performing a colony count on a urine culture, 1 colony type is what? |
|
Definition
|
|
Term
| When performing a colony count on a urine culture, 3 or more colony types in equal numbers is what? |
|
Definition
|
|
Term
| Urine Colony Count - If using a 1 μL loop, multiply the number of colonies by what? |
|
Definition
|
|
Term
| Urine Colony Count - If using a 10 μL loop, multiply the number of colonies by what? |
|
Definition
|
|
Term
| After multiplying by 1000 for 1 μL or 100 for 10 μL for a Urine Colony Count then what do you multiply it by? |
|
Definition
|
|
Term
| If a urine colony count is 100,000 or more, what will you report it as? |
|
Definition
|
|
Term
| When processing throat swabs for a GAS culture, the medium of choice is: |
|
Definition
|
|
Term
| Would you use BCYE with Legionella species? |
|
Definition
|
|
Term
| What primary media battery would you use for Sputum? |
|
Definition
|
|
Term
| Which of the following is the most appropriate method for collecting a urine specimen from a patient with an indwelling catheter? |
|
Definition
| Aspirate urine aseptically from the catheter tubing |
|
|
Term
| The lab receives a sputum specimen for routine culture. The direct Gram stain shows a predominance of pleomorphic GNB. The next day there is no growth on the BAP, but growth on the CHOC agar. What potential pathogen do you suspect? |
|
Definition
|
|
Term
|
Definition
| Presence of bacteria in the bloodstream |
|
|
Term
|
Definition
| Infection of the endocardium or heart valves |
|
|
Term
| What are 2 causes for/of Endocarditis |
|
Definition
Previously damaged heart valves
Artificial heart valves |
|
|
Term
|
Definition
| Usually occurs as a result of continuous bacteremia |
|
|
Term
|
Definition
actively dividing bacteria
their harmful by-products
activation of the body’s inflammation and immune response |
|
|
Term
| Describe Transient Bacteremia |
|
Definition
| Brushing teeth & surgery in infected site |
|
|
Term
| Describe Intermittent Bacteremia |
|
Definition
| Abscess that periodically leaks |
|
|
Term
| Describe Constant Bacteremia |
|
Definition
| Endocarditis or infected intravascular site |
|
|
Term
If a doctor suspects a patient of having:
Acute primary bacteremia or fungemia
Meningitis, osteomyelitis, arthritis or pneumonia,
What should you collect? |
|
Definition
2 or 3 sets of blood cultures
One right after the other from different sites |
|
|
Term
| If a doctor suspects fever of uncertain origin, what should you collect? |
|
Definition
2 or 3 sets of blood cultures
One right after the other from different sites |
|
|
Term
| If a doctor suspects fever of uncertain origin and the cultures are negative after 24-48 hours, what should you do? |
|
Definition
| collect 2 more sets of blood cultures |
|
|
Term
True or False:
BC should be obtained ASAP!
after the onset of fever or chills
prior to receiving antimicrobial therapy |
|
Definition
|
|
Term
True or False:
Both the skin and BC bottles should be disinfected |
|
Definition
|
|
Term
True or False:
Blood from an IV catheter should not be drawn if peripheral site available |
|
Definition
|
|
Term
True or False:
If limb with IV catheter is used:
Blood should be drawn below the catheter |
|
Definition
|
|
Term
True or False:
If limb with IV catheter is used:
Blood should be drawn above the catheter |
|
Definition
|
|
Term
| By drawing a blood sample above a IV catheter, what does this ensure? |
|
Definition
| Ensures blood collection is not diluted with fluid being infused |
|
|
Term
| What is the Single MOST Important Variable in recovering MOs? |
|
Definition
|
|
Term
| What is the volume of blood typical for adults? |
|
Definition
|
|
Term
| What is the volume of blood typical for children? |
|
Definition
|
|
Term
True or False:
Some BC bottles require a dilution (1:5 to 1:10) |
|
Definition
|
|
Term
| What is the anticoagulant in BC bottles? |
|
Definition
|
|
Term
| What does SPS do in BC Bottles? |
|
Definition
Inactivates PMNs
Inhibits serum complement |
|
|
Term
| What does Biphasic BC media improve recovery of? |
|
Definition
|
|
Term
| What is the incubation environment for BC? |
|
Definition
35o C
CO2 system is in each bottle |
|
|
Term
True or False:
Length of BC incubation depends on system
Usually 5-10 days |
|
Definition
|
|
Term
| How long are BCs generally incubated for? |
|
Definition
|
|
Term
What is the following called:
Subculture all bottles
At 6-12 hrs & 48 hrs |
|
Definition
|
|
Term
| What stain do you use for blind gram staining |
|
Definition
|
|
Term
| BC + Cultures can be detected in how many days? |
|
Definition
|
|
Term
| Newer systems measure BC CO2 using what? |
|
Definition
Infrared spectrophotometry
Fluorescent sensor
Colorimetric sensors
BacTec, BacT/Alert |
|
|
Term
| Describe BacT/Alert System |
|
Definition
| Colorimetric sensor in bottle measures CO2 |
|
|
Term
| Describe Manometric System |
|
Definition
| monitors gas production/consumption |
|
|
Term
| Describe Lysis Centrifugation System |
|
Definition
Centrifuge blood tube and inoculate agar plates
Incubate and examine for growth
Isolator tubes |
|
|
Term
| For Positive Cultures, Subculture Blood onto what if GNB are seen? |
|
Definition
|
|
Term
| For Positive Cultures, Subculture Blood onto what if GPC are seen? |
|
Definition
|
|
Term
| For Positive Cultures, what does the Join Commission require? |
|
Definition
| tech to verify the test result by having the person read back the information |
|
|
Term
| For Positive Cultures, Should you notify the physician? |
|
Definition
|
|
Term
True or False:
When reporting a blood culture gram stain, it is important to indicate morphological characteristics |
|
Definition
|
|
Term
What is used to rapidly ID the following MOs:
S. aureus, MRSA, E. faecalis, & Candida albicans |
|
Definition
|
|
Term
True or False:
AdvanDx uses FISH (fluorescence in situ hybridization) assay that uses PNA (peptide nucleic acid) probes |
|
Definition
|
|
Term
| What type of grower is Brucella? |
|
Definition
|
|
Term
| With the manual method how long do you hold Brucella bottles? |
|
Definition
|
|
Term
| With the automated method how long do you hold Brucella bottles? |
|
Definition
|
|
Term
| What do you subculture Brucella specimens to? |
|
Definition
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|
Term
| Some MOs are always considered significant, Name 2 |
|
Definition
|
|
Term
| Common Skin Contaminants, Name 4 |
|
Definition
Bacillus species
Corynebacterium species
Propionibacterium acnes
Coagulase negative staphylococcus |
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|
Term
How many bottles positive?
1 positive bottle equals |
|
Definition
|
|
Term
How many bottles positive?
≥ 2 positive bottles equals |
|
Definition
|
|
Term
How many different kinds of organisms present?
1 MO |
|
Definition
|
|
Term
How many different kinds of organisms present?
≥ 2 MOs |
|
Definition
|
|
Term
| With IV Catheters, what are cultured? |
|
Definition
| Both blood from the IV and the catheter tip |
|
|
Term
| How do you culture the IV Catheter tip? |
|
Definition
Roll catheter onto a BAP
Count number of colonies |
|
|
Term
| With the IV Catheter tip, how many colonies are considered significant? |
|
Definition
|
|
Term
True or False:
A positive catheter culture and blood culture are required for diagnosis |
|
Definition
|
|
Term
| What is the principle of operation of the BacT/Alert blood culture system? |
|
Definition
| CO2 production that changes the color of the colorimetric sensor of the bottle |
|
|
Term
| The proper blood-to-broth ratio for BCs to reduce the antibacterial effect of serum in adults is: |
|
Definition
|
|
Term
| SPS is used as an anticoagulant for BCs because it... |
|
Definition
| inactivates neutrophils & components of serum complement |
|
|
Term
| What is required to diagnosis a catheter-related infection? |
|
Definition
| > or = 15 colonies on BAP + positive BCs |
|
|
Term
| Three sets of BCs were obtained from a patient with fever & suspected endocarditis. The aerobic bottle of one set had growth of S. epidermidis at 5 days of incubation. This indicates that: |
|
Definition
| The organism is most likely a contaminant |
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