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complete genome sequence in..... |
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1.double lipid bilayer = nuclear envelope 2.continuous with rER 3.rER lumen continuous with perinuclear space 4.nuclear lamina=layer of inermediate filaments |
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greater than 100 proteins facilitated and active |
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Nuclear Localization Signal never cleaved |
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1.Mitotic 2.Gap 1 3.DNA Synthesis 4.Gap 2 |
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Prophase Metaphase Anaphase Telophase/Cytokinesis |
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G1 restriction influences |
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GF nutrients cell size DNA damage (stop will go to G0) |
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G2-M restriction influences |
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cell size DNA damage DNA replication (if not synthesized will stop here) |
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Meta-Ana restriction influences |
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chromosome attachment to spindle |
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Cyclin dependent kinase active when mitotic cyclin present stimulates nuclear envelope breakdown,chromosome condensation, mitotic spindle formation, protein degradation |
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activated by G1 cyclin activates kinases can't activate will go to G0 |
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Program cell death sequence |
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1.chromosome condense/cyto shrink 2.nucleus fragement/DNA digested/cyto fragments/blebs 3.phagocytosis |
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no specificity requires primer move 3'->5' elongation 5'->3' |
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left side: top continuous/bottom lagging right side: top lagging/bottom continuous lagging=okazaki fragments |
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small = 30s large = 50s exit site peptidyl site aminoacyl site |
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anticodon brings in correct codon with help of ___________? |
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aminoacyl-tRNA synthetase |
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3 base pairing has flexibility at 5' (inosine used is modified base) |
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ribsome binding site on the mRNA |
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proteins facilitate placement of next tRNA and requires GTP |
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enzyme helping the placement of the P site aa on the A site aa comes from the ribsome |
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release factor with GTP and blows all apart |
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regulatory proteins including transcription factors |
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2 categories of transcription factors |
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1. general TF = needed in all cells Na/K, clathrin, tubulin...... 2. regulatory TF = specific proteins |
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hereitable changes in gene expression or cell phenotype not caused by changes in the DNA sequence
1.DNA methylation 2.histone modification |
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