Term
What is the Gram Stain for Legionella? |
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Definition
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Term
Are Legionella species Aerobic or Anaerobic? |
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Definition
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Term
How are Legionella species transmitted? |
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Definition
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Term
What are the normal habitats for the Legionella species? |
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Definition
Soil, water, Water cooling towers, showers |
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Term
What is an infection caused by Legionella called? |
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Definition
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Term
Legionella pneumophila Acute pneumonia is also known as what? |
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Definition
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Term
Legionella pneumophila Nonpneumonia febrile illness is also known as what? |
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Definition
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Term
True or False: Pontiac Fever is associated with "flu-like" symptoms |
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Definition
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Term
True or False: Legionnaire's Disease is associated with High fever/Prostration |
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Definition
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Term
What is the most sensitive method for diagnosis of Legionellosis? |
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Definition
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Term
You should process a potential Legionella specimen within how many minutes? |
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Definition
30 minutes Otherwise refrigerate/freeze |
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Term
You should perform the following when you believe you have what type of MO: Acid decontamination Dilue most specimens to dilute out inhibitory substances |
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Definition
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Term
Legionella - May use nonselective BCYE only for what type of specimens? |
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Definition
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Term
What is on BCYE or BCYE-alpha that the Legionella organism requires? |
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Definition
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Term
True or False: Legionella will grow on BA |
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Definition
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Term
True or False: Legionella will NOT grow on BA |
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Definition
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Term
True or False: Legionella needs humidity NOT CO2 |
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Definition
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Term
How many days does it take to grow Legionella? |
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Definition
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Term
Identify the following: No growth on BA Growth on BCYE No growth on BCYE lacking cysteine |
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Definition
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Term
How would you confirm the identity of Legionella? |
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Definition
Nucleic acid probe, DFA, or slide agglutination |
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Term
Bacterial antigen test detects Legionella pneumophila serogroup _____ antigen in urine |
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Definition
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Term
Indirect Fluorescence ABY test is diagnostic use for what? |
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Definition
L. pneumophila serogroup 1 only |
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Term
Indirect Fluorescence ABY test for L. pneumophila serogroup 1 only needs what 2 types of sera? |
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Definition
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Term
What is the Gram Stain for Bordetella? |
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Definition
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Term
What are the 3 Whooping cough stages? |
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Definition
Catarrhal Paroxysmal Convalescent |
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Term
Which Whooping cough stage is characterized by nonspecific symptoms? |
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Definition
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Term
Which Whooping cough stage is characterized by whoop on inspiration? |
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Definition
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Term
What is the method of choice for direct detection of B. pertussis and B. parapertussis? |
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Definition
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Term
True or False: B. pertussis - use a special transport medium if not plated at bedside |
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Definition
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Term
True or False: Generally, B. pertussis will NOT grow on BAP |
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Definition
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Term
What are the media that are generally used to culture B. pertussis? |
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Definition
Bordet-Gengou (Potato-Glycerol-Blood) Charcoal Blood Agar (Regan-Lowe) |
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Term
What are the characteristic colonies of Bordetella on Bordet-Gengou? |
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Definition
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Term
How long may it take for "mercury drop" colonies to form on Bordet-Gengou? |
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Definition
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Term
What type of incubation does Bordetella need? |
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Definition
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Term
Identify the following: No Growth on BA Special B. pertussis agar: Growth Biochemical tests not helpful Serological test: DFA or agglutination |
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Definition
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Term
What is the gram stain of Gardnerella vaginalis? |
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Definition
Gram variable to GN Coccobacilli |
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Term
The cell wall of Gardnerella vaginalis has what type of gram characteristic? |
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Definition
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Term
What is the disease caused by Gardnerella vaginalis that is characterized by foul-smelling vaginal discharge? |
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Definition
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Term
True or False: G. vaginalis is considered normal flora |
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Definition
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Term
True or False: G. vaginalis - male partners generally do not need to be treated |
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Definition
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Term
Name 1 other infection caused by G. vaginalis |
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Definition
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Term
What is the most important test in diagnosis of bacterial vaginosis? |
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Definition
Direct Exam for Clue Cells |
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Term
The 4 signs listed below are diagnostic of what disease: Vaginal pH > 4.5 Thin homogeneous vaginal discharge: milk-like consistency Presence of clue cells Positive sniff test: Vaginal secretions + 10% KOH --> fishy smell |
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Definition
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Term
True or False: A culture is not recommended for diagnosing Bacterial vaginosis |
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Definition
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Term
True or False: A culture is appropriate for extravaginal sites |
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Definition
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Term
If cultured, what type of hemolysis does G. vaginalis have on the following media: V agar or HBT |
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Definition
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Term
If cultured, what type of hemolysis does G. vaginalis have on the following media: Sheep BA |
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Definition
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Term
What type of agar does the following occur on: G. vaginalis: Purple --> Yellow when substance is hydrolyzed |
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Definition
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Term
What environment is needed in order to incubate G. vaginalis? |
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Definition
CO2 35 degrees Celsius 48 hours |
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Term
What type of growth is attributed to G. vaginalis |
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Definition
Slow growth/tiny colonies |
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Term
What is the Gram Stain of Pasteurella multocida? |
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Definition
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Term
What is the normal habitat of Pasteurella multocida? |
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Definition
Upper respiratory tract of dogs & cats |
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Term
True or False: Pasteurella multocida grows well on BA & CHOC |
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Definition
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Term
True or False: Pasteurella multocida does NOT grow well on MAC |
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Definition
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Term
What is the characteristic odor of Pasteurella multocida? |
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Definition
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Term
What is the preferred environment for incubation of Pasteurella multocida? |
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Definition
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Term
Identify the following: Oxidase + Catalse + Indole + |
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Definition
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Term
Are Most AACEK aerobes or anaerobes? |
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Definition
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Term
What do AACEK MOs require? |
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Definition
CO2 or are stimulated by its presence |
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Term
Are AACEK MOs fast or slow growing? |
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Definition
Slow growing 48-72 hours for adequate growth |
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Term
True or False: AACEK - No growth on selective enteric agar (MAC or EMB) |
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Definition
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Term
True or False: AACEK - optimal growth on BA or CHOC |
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Definition
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Term
Due to AACEK having predilection for attachment to heart valves (usually damaged or prosthetic) they are an important cause of what infection/disease/illness? |
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Definition
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Term
What are the 2 A organisms in AACEK? |
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Definition
Aggregatibacter aphrophilus Aggregatibacter actinomycetemcomitans |
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Term
What is the C MO in AACEK? |
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Definition
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Term
What is the E MO in AACEK? |
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Definition
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Term
What is the K MO in AACEK? |
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Definition
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Term
Which MO has a colony star-shaped configuration |
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Definition
Aggregatibacter actinomycetemcomitans |
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Term
In addition to endocarditis, what else does Aggregatibacter actinomycetemcomitans cause? |
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Definition
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Term
Which MO tends to form rosettes on gram stain? |
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Definition
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Term
What do 50% of isolates of Eikenella corrodens do to the agar? |
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Definition
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Term
Which MO is connected with bites or trauma associated with fist fights? |
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Definition
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Term
Identify the following: Oxidase + Catalase = "Bleach" odor |
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Definition
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Term
What is the odor that is characterized with Eikenella corrodens? |
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Definition
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Term
Is Kingella Oxidase Positive or Negative? |
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Definition
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Term
Does Kingella ferment glucose? |
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Definition
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Term
Which MO goes with the following description: Propensity for bone & joint infections in young children |
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Definition
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Term
Which of the AACEK MOs can grow on MTM? |
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Definition
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Term
Kingella denitrificans may be confused with what other MO? |
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Definition
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Term
How do you differentiate Kingella denitrificans from Neisseria gonorrhoeae? |
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Definition
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Term
Is Kingella denitrificans Catalase Positive or Catalase Negative? |
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Definition
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Term
Is Neisseria gonorrhoeae Catalase Positive or Catalase Negative? |
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Definition
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Term
What is Capnocytophaga associated with? |
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Definition
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Term
What is the gram stain of Capnocytophaga? |
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Definition
Fusiform, filamentous GNB |
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Term
Is Capnocytophaga fast growing or slow growing? |
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Definition
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Term
True or False: Capnocytophaga will grow on BAP or CHOC |
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Definition
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Term
True or False: Capnocytophaga will NOT grow on MAC |
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Definition
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Term
Is Capnocytophaga aerobic or anaerobic? |
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Definition
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Term
What type of motility is characteristic of Capnocytophaga? |
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Definition
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Term
Presumptively Identify the following: Yellow colonies Fusiform, filamentous GNB Gliding Motility |
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Definition
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Term
What is the zoonotic Capnocytophaga organism associated with dog/cat bites or contact? |
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Definition
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Term
What is the zoonotic Capnocytophaga organism associated with dog bites? |
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Definition
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Term
What is the disease that is attributed to Brucella? |
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Definition
Brucellosis (Undulant Fever) |
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Term
Brucella is one cause of what suisillness/disease? |
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Definition
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Term
True or False: Brucella is a type of Zoonosis |
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Definition
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Term
How is Brucella transmitted? |
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Definition
Meat Packers Associated with unpasteurized milk Animals to man |
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Term
Match the species that infect humans with the animal that they come from: B. suis |
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Definition
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Term
Match the species that infect humans with the animal that they come from: B. abortus |
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Definition
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Term
Match the species that infect humans with the animal that they come from: B. melitensis |
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Definition
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Term
Match the species that infect humans with the animal that they come from: B. canis |
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Definition
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Term
Name 2 specimens that Brucella is most often isolated from |
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Definition
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Term
What BSL Level is Brucella? |
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Definition
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Term
True or False: All Brucella cultures should be taped shut during incubation |
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Definition
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Term
What environment is needed for Brucella species? |
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Definition
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Term
You should hold Brucella Blood Cultures for how many days when using nonautomated BC systems |
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Definition
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Term
You should hold Brucella Blood Cultures for how many days when using automated BC systems |
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Definition
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Term
What is the Gram Stain of Brucella? |
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Definition
GN Coccobacilli; Appears like "fine sand" |
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Term
True or False: Brucella grows quickly on BAP & CHOC |
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Definition
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Term
True or False: Brucella grows slowly on BAP & CHOC |
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Definition
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Term
At 48 hours, what does Brucella look like on BAP or CHOC? |
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Definition
Non-hemolytic Non-pigmented |
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Term
What % of Brucella will grow on MAC? |
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Definition
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Term
Is Brucella Oxidase Positive or Negative? |
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Definition
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Term
Is Brucella Urea Positive or Negative? |
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Definition
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Term
Is Brucella Satellite Positive or Negative? |
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Definition
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Term
Is Brucella canis Oxidase Positive or Negative? |
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Definition
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Term
True or False: Serology & NAAT can also be used to ID Brucella |
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Definition
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Term
Which MO causes Tularemia, aka Rabbit Fever? |
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Definition
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Term
The following clinical syndromes are characteristic of what MO? Pustule at site of infection Enlarged lymph nodes Bacteremia Pneumonia Oculoglandular form |
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Definition
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Term
What is the BSL of Francisella tularensis? |
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Definition
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Term
What does Francisella tularensis require in order to be cultured? |
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Definition
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Term
What is the recommended media for Francisella tularensis? |
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Definition
Glucose-cysteine blood agar + thiamin |
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Term
True or False: Francisella tularensis can grow on CHOC, BCYE, BA, & MTM |
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Definition
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Term
Francisella tularensis Contaminated specimens are best cultivated on what type of media? |
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Definition
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Term
True or False: Francisella tularensis is uncommonly isolated from blood cultures |
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Definition
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Term
Is Francisella tularensis an aerobe or an anaerobe? |
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Definition
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Term
Is CO2 required for Francisella tularensis to grow? |
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Definition
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Term
What is the gram stain of Francisella tularensis? |
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Definition
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Term
What type of growth will you find on BAP after 48 hours for Francisella tularensis? |
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Definition
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Term
Francisella tularensis - 1 to 2 mm grayish white colonies on CHOC at how many hours? |
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Definition
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Term
Presumptively Identify the following: Oxidase - Negative Catalase - Weak Positive B-lactamase - Positive Satellite - Negative Urea - Negative |
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Definition
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Term
What disease is Streptobacillus moniliformis associated with? |
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Definition
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Term
What MO, other than Streptobacillus moniliformis, also causes Rat Bite Fever? |
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Definition
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Term
What does Streptobacillus moniliformis require in order to be cultured? |
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Definition
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Term
In a Broth Culture what does Streptobacillus moniliformis look like? |
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Definition
"Fluff balls" / "Bread Crumbs" |
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Term
What is the Gram Stain of Streptobacillus moniliformis? |
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Definition
Pleomorphic GNB (Rounded & Pointed ends) |
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Term
Is Chromobacterium violaceum an aerobe or an anaerboe? |
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Definition
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Term
What is the habitat of Chromobacterium violaceum? |
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Definition
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Term
What is a characteristic look of Chromobacterium violaceum? |
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Definition
Distinctive Purple Pigment |
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Term
What disease is associated with Chromobacterium violaceum? |
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Definition
Contaminated wounds (ex. motorcycle accident) |
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