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Exam 3 Material
Materials covered in class outside of glycolysis and gluconeogenesis
115
Biochemistry
Undergraduate 4
03/30/2013

Additional Biochemistry Flashcards

 


 

Cards

Term
what percentage of the cell membrane is cholesterol
Definition
18-20%
Term
What are the 5 types of transport proteins
Definition
Integral, Embedded, Associated, Peripheral, Anchored
Term
Which type of transport protein is most common
Definition
Integral
Term
What type of transport protein is least common
Definition
Embedded
Term
What two types of transport proteins help each other
Definition
Associated and peripheral
Term
what kind of transport protein is typically a fatty acid
Definition
anchored
Term
what are the 4 main function of the cell membrane
Definition
barrier/protection; transport; enzymatic reactions (catalysis); receptor (for biological activity)
Term
what molecules are considered small molecules
Definition
O2; N2; CO2
Term
What two types of transport can be used for small molecules
Definition
diffusion and facilitated diffusion
Term
What are the two categories of transportation
Definition
Active and Passive transportations
Term
What kind of concentration gradient is associated with passive transport
Definition
high to low (with the gradient)
Term
what kind of concentration gradient is associated with active transport
Definition
low to high (against gradient)
Term
what vitamins are lipid soluble
Definition
Vitamins A, D, E, K
Term
Arachidonic acid is the main precursor to what two types of compounds
Definition
prostoglandins and leukotrins
Term
what are the two precursors for a nucleic acid (in order)
Definition
nucleoside and then nucleotide
Term
what are nucleosides made up of
Definition
a base and a sugar covalently bonded
Term
what are nucleotides made up of
Definition
a base, a sugar, a phosphoric acid residue covalently bonded
Term
what are the two types of bases in nucleic acids
Definition
purines and pyrimidines
Term
what are the purines
Definition
two ring structures; adenine and guanine
Term
what are the pyrimidines
Definition
one ring structures; cytosine, thymine, uracil
Term
what are the 2 main types of sugars used in nucleic acids
Definition
D-ribose; 2-deoxy-D-ribose
Term
how many levels are there of nucleic acids
Definition
4 levels
Term
what is the 1st level of nucleic acid
Definition
order of bases forming a polynucleotide (specific genetic code)
Term
what is the 2nd level of nucleic acid
Definition
3D conformation
Term
what is the 3rd level of nucleic acid
Definition
Supercoiling
Term
what is the 4th level of nucleic acid
Definition
interactions between DNA and proteins
Term
what the characteristics of the shape of the 2nd level structure
Definition
a double helix with 2 polynucleotide chains that run antiparallel to each other with a phosphate-sugar backbone with bases perpendicular to the axis
Term
what are the three types of DNA
Definition
B-DNA; A-DNA; Z-DNA
Term
What are the characteristics of B-DNA
Definition
the normal DNA; 10 bases per turn; right handed turns
Term
what are the characteristics of A-DNA
Definition
11 bases per turn; bases not perpendicular to axis; right handed turns
Term
what are the characteristics of Z-DNA
Definition
10 bases per turn; left handed turn; involved with gene expression; looks similar to B-DNA
Term
What happens in the 3rd level of nucleic acids
Definition
ends of DNA form a phosphodiester bond forming a circular DNA and then it supercoils
Term
what are the two types of supercoiling
Definition
Positive (overwinding); Negative (underwinding)
Term
What enzymes is involved in supercoiling in prokaryotes
Definition
Topoisomerase I and II
Term
what does topoisomerase I do in prokaryote supercoiling
Definition
cuts phosphodiester backbone of strands; reseals
Term
what does topoisomerase II do in prokaryote supercoiling
Definition
cuts both strands; reseals
Term
what enzyme does topoisomerase II behave like
Definition
DNA gyrase
Term
what happens in supercoiling in eukaryotes
Definition
chromatin --> principle protein of histones
Term
what are the 5 types of histones
Definition
H1; H2A; H2B; H3; H4
Term
how many H-bonds does an A-T base pair make
Definition
2
Term
how many H-bonds does a C-G base pair make
Definition
3
Term
what are the two precursors for a nucleic acid (in order)
Definition
nucleoside and then nucleotide
Term
what are nucleosides made up of
Definition
a base and a sugar covalently bonded
Term
what are nucleotides made up of
Definition
a base, a sugar, a phosphoric acid residue covalently bonded
Term
what are the two types of bases in nucleic acids
Definition
purines and pyrimidines
Term
what are the purines
Definition
two ring structures; adenine and guanine
Term
what are the pyrimidines
Definition
one ring structures; cytosine, thymine, uracil
Term
what are the 2 main types of sugars used in nucleic acids
Definition
D-ribose; 2-deoxy-D-ribose
Term
how many levels are there of nucleic acids
Definition
4 levels
Term
what is the 1st level of nucleic acid
Definition
order of bases forming a polynucleotide (specific genetic code)
Term
what is the 2nd level of nucleic acid
Definition
3D conformation
Term
what is the 3rd level of nucleic acid
Definition
Supercoiling
Term
what is the 4th level of nucleic acid
Definition
interactions between DNA and proteins
Term
what the characteristics of the shape of the 2nd level structure
Definition
a double helix with 2 polynucleotide chains that run antiparallel to each other with a phosphate-sugar backbone with bases perpendicular to the axis
Term
what are the three types of DNA
Definition
B-DNA; A-DNA; Z-DNA
Term
What are the characteristics of B-DNA
Definition
the normal DNA; 10 bases per turn; right handed turns
Term
what are the characteristics of A-DNA
Definition
11 bases per turn; bases not perpendicular to axis; right handed turns
Term
what are the characteristics of Z-DNA
Definition
10 bases per turn; left handed turn; involved with gene expression; looks similar to B-DNA
Term
What happens in the 3rd level of nucleic acids
Definition
ends of DNA form a phosphodiester bond forming a circular DNA and then it supercoils
Term
what are the two types of supercoiling
Definition
Positive (overwinding); Negative (underwinding)
Term
What enzymes is involved in supercoiling in prokaryotes
Definition
Topoisomerase I and II
Term
what does topoisomerase I do in prokaryote supercoiling
Definition
cuts phosphodiester backbone of strands; reseals
Term
what does topoisomerase II do in prokaryote supercoiling
Definition
cuts both strands; reseals
Term
what enzyme does topoisomerase II behave like
Definition
DNA gyrase
Term
what happens in supercoiling in eukaryotes
Definition
chromatin --> principle protein of histones
Term
what are the 5 types of histones
Definition
H1; H2A; H2B; H3; H4
Term
how many H-bonds does an A-T base pair make
Definition
2
Term
how many H-bonds does a C-G base pair make
Definition
3
Term
what are characteristics of RNA
Definition
long, unbranched chain of polynucleotides; phosphodiester bonds; B-D-ribose sugar; generally single stranded
Term
what is RNA base sequence determine by
Definition
DNA through transcription
Term
what bases are used in RNA
Definition
Adenine, cytosine, guanine, uracil
Term
What are the 6 types of RNA
Definition
tRNA; rRNA; mRNA; snRNA; miRNA; siRNA
Term
what is the size and function of tRNA
Definition
small; transport amino acids to site of protein ribosome
Term
what is the size and function of rRNA
Definition
varies in size; combines with proteins to form ribosomes
Term
what is the size and function of mRNA
Definition
varies in size; directs amino acid sequence of protein and carries the genetic code
Term
what is the size and function of snRNA
Definition
small; (small nuclear) processes initial mRNA to maturity
Term
what is the size and function of miRNA
Definition
small; (micro)gene expression, growth and development
Term
what is the size and function of siRNA
Definition
small; (small-interfering) controls gene expression, knock out genes
Term
what are characteristics of RNA
Definition
long, unbranched chain of polynucleotides; phosphodiester bonds; B-D-ribose sugar; generally single stranded
Term
what is RNA base sequence determine by
Definition
DNA through transcription
Term
what bases are used in RNA
Definition
Adenine, cytosine, guanine, uracil
Term
What are the 6 types of RNA
Definition
tRNA; rRNA; mRNA; snRNA; miRNA; siRNA
Term
what is the size and function of tRNA
Definition
small; transport amino acids to site of protein ribosome
Term
what is the size and function of rRNA
Definition
varies in size; combines with proteins to form ribosomes
Term
what is the size and function of mRNA
Definition
varies in size; directs amino acid sequence of protein and carries the genetic code
Term
what is the size and function of snRNA
Definition
small; (small nuclear) processes initial mRNA to maturity
Term
what is the size and function of miRNA
Definition
small; (micro)gene expression, growth and development
Term
what is the size and function of siRNA
Definition
small; (small-interfering) controls gene expression, knock out genes
Term
what are the building blocks of carbohydrates
Definition
monosaccharides
Term
what are the 4 main purposes of carbohydrates
Definition
energy; glycolipids; structural backbone of nucleic acid; composition (plants-->cell wall)
Term
what creates the D conformation
Definition
the OH group on the right side
Term
what creates the L conformation
Definition
the OH group on the left side
Term
which conformation is biologically active
Definition
D conformation
Term
which sugar is the simplest
Definition
sucrose
Term
which sugar is the most important in diet
Definition
glucose
Term
which sugar is the sweetest
Definition
fructose
Term
which sugar is known as milk sugar
Definition
galactose
Term
which sugar is in nucleic acids
Definition
ribose
Term
what creates the alpha configuration
Definition
the OH group is down
Term
what creates the beta configuration
Definition
the OH group is up
Term
what kind of reaction provides energy
Definition
oxidation
Term
what does Fehling's reaction do
Definition
it tests for reducing sugar
Term
what do aldehydes oxidize into
Definition
carboxylic acid
Term
what are the four main reactions of monosaccharides
Definition
Redox; esterifications of sugars; amino drivatives; glycosidic formation
Term
what is the difference between oligosaccharides and polysaccharides
Definition
oligo binds 10-12 units while poly binds 100+
Term
what are characteristics of O-glycosidic bonds
Definition
oxygen involved; links sugars to one another
Term
what are characteristics of N-glycosidic bonds
Definition
nitrogen involved; links sugar to nucleic acids
Term
what are the 3 most abundant disaccharides
Definition
1)lactose; 2)sucrose; 3)maltose
Term
what makes up lactose
Definition
galactose + galactose [beta(1,4)]
Term
what makes up sucrose
Definition
glucose + fructose [alpha(1,2)]
Term
what makes up maltose
Definition
glucose + glucose [alpha(1,4)]
Term
what are the three main functions of polysaccharides
Definition
storage polysac (energy storage and starch glycogen); structural polysac(protective cell wall, cellulose and chitin); structural peptidoglycans (bacterial cell walls)
Term
what are the two types of polysaccharides
Definition
homopolysaccharides and heteropolysaccharides
Term
what are the characteristics of homopolysaccharides
Definition
one type of monosaccharides; either unbranched (linear) or branched
Term
what are the characteristics of heterpolysaccharides
Definition
multiple types of monosaccharides; 2 unbranched, >2 branched
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