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exam 3
questions from spring 09
34
Biochemistry
Undergraduate 4
11/21/2010

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Term
The most naturally abundant aldohexoses are... (name 3)
Definition
glucose, mannose and galactose
Term
The disaccharide sucrose is composed of...
Definition
glucose and fructose; alpha beta (1->2)
Term
Plants store energy in the form of the polysaccharide...
Definition
starch
Term
The exoskeleton of insects and lobsters is composed of...
Definition
Chitin
Term
Functions of the carbohydrates on glycoproteins include... (there are 3)
Definition
1)Cell-surface identification markers
2)Blood clotting
3)Protein turnover
Term
Name the 3 redox pairs
Definition
1)NAD+/NADH
2)NADP+/NADPH
3)FAD/FADH2
Term
In thermodynamic terms, when ATP and H2O react to form ADP and Pi, the procucts are at a lower energy level than the reactants (more stable) due to what?
Definition
Resonance stabilization and charge repulsion
Term
What are the three primary metabolic fates of pyruvate?
Definition
Ethanol, acetyl-CoA, and lactate
Term
The synthesis of glucose from smaller precursors is called...
Definition
Gluconeogenesis
Term
The enzyme, _____ uses _____ to cleave glycogen (at reducing end) and forms glucose-1-phosphate.
Definition
Glycogen phosphorylase, Pi
Term
The 3 steps in glycolysis that are different than those in gluconeogenesis are catalyzed by these enzymes... (3)
Definition
pyruvate kinase, phosphofructokinase, hexokinase
Term
Complete the following reaction (pyruvate Dehydrogenase complex)
pyruvate + CoASH + NAD+ ->
Definition
Acetyl-CoA + NADH + H+ + CO2
Term
The approximate yield of ATP from complete oxidation of glucose is...
Definition
1)36 ATP if the glycerol-3-phosphate shuttle is used.
2)38 ATP if the malate-aspartate shuttle is used.
Term
What is the enzyme that commits glucose to glycolysis?
Definition
Phosphofructokinase
Term
Compared with the resting state, vigorously contracting muscle shows...
Definition
an increased conversion of pyruvate to lactate
Term
What are 4 sugars that would give a positive Benedict's test?
Definition
1)Glucose
2)Galactose
3)Lactose
4)Fructose
Term
What monosaccharides can be used to fuel the glycolysis pathway?
Definition
Glucose, fructose, and galactose
Term
The cost in high-energy phosphate bonds for the formation of 1 mole glucose from lactate (gluconeogenesis) is...?
Definition
6 moles of ATP/GTP
Term
In bacterial cell walls...
Definition
Polysaccharides are hydrogen bonded together.
Term
The reduction of pyruvate to lactate...
Definition
allows the recycling of NAD+
Term
The oxidation reactions of the Pentose phosphate pathway...
Definition
produce NADPH rather than NADH
Term
The citric acid cycle is considered amphibolic because it...
Definition
plays a major role in both anabolism and catabolism
Term
Lactose intolerance ...
Definition
Arises from inability to metabolize disaccharide lactose.
Term
The reactive portion of coenzyme A is...
Definition
a thioester group
Term
A cell in an active metabolic state has...
Definition
a low ATP/ADP and a low NADH/NAD+ ratio
Term
The Glyoxylate cycle allow the organism that posses this pathway to...
Definition
Produce carbohydrates from fats
Term
The CAC is considered part of the aerobic metabolism even theough oxygen does not appear explicitly in any reaction b/c...
Definition
The NADH and FADH2 produced are reoxidized in the electron transport chain linked to O2
Term
Blood typing dependson on...
Definition
The nature of the oligosaccharide portion of glycoproteins on the surface of red blood cells.
Term
List 4 facts about cellulose.
Definition
1)It is a homopolymer of D-glucose units 2)Glucose units are linked by b(1->4) glycosidic bonds 3)The cellulose structure is stabilized by intramolecular hydrogen bonds
Term
What is important to do concerning gluconeogenesis?
Definition
It is important to maintain blood glucose levels during the normal overnight fast (sleep)
Term
List 2 biochemical differences between catabolism and anabolism.
Definition
Catabolism- oxidation,exergonic, degradation of biomolecules.
Anabolism- Reduction, endergonic, synthesis of biomolecules.
Term
List 2 differences between amylose vs glycogen.
Definition
Amylose- Found in plants; main nutrient storage molecule not branched
Glycogen- Found in animals, main nutriendt storage molecule branched.
Term
_____ are oxygenasesw that metabolize various nutrient substates and also detoxify drugs/xenobiotics to allow easier elimination from the body.
Definition
Cytochromes
Term
ATP synthesis by direct phosphoryl group transfer from a reactive intermediate is called...
Definition
substrate phosphorylation
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