Term
| When ABY is bound to the carrier molecule and used to detect AGN this technique is known as: |
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Definition
| Reverse passive agglutination |
|
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Term
Which of the following procedures is the MOST sensitive?
Countercurrent immunoelectrophoresis
Immunoassays |
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Definition
|
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Term
What is the following an example of?
Titer = 1:2, 1:4, 1:8, 1:16, 1:32, 1:64
Titer : = = = 3+ 3+ 2+ |
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Definition
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Term
The following results were obtained on a newborn serum test for exposure to Toxoplasma:
Toxoplasma IgG: Negative
Toxoplasma IgM: Positive
These results indicate: |
|
Definition
| The child has current infection due to congenital toxoplasmosis |
|
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Term
Is the following a fourfold rise in titer?
1:2 ---> 1:8 |
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Definition
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Term
[image]
Which area of the curve shows no agglutination formed due to AGN excess? |
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Definition
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Term
| Hybridoma cells used to produce specific monoclonal ABY are made by the fusion of: |
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Definition
| Plasma cells and myeloma cells |
|
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Term
| AGN derived from different individuals from the same species are BEST described as: |
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Definition
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Term
| A child is given the measles vaccine and produces measles ABYs. This type of immunity is best described as: |
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Definition
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Term
| T cells which respond to self AGNs are usually destroyed by the: |
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Definition
|
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Term
| The carrier particles in coagglutination tests are: |
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Definition
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Term
1&4 - Known Blastomyces + serum (control)
2 - patient J. Jones serum
3 - patient D. Brown serum
4 - Known Blastomyces AGN
It may be concluded that the patient in well #2:
[image] |
|
Definition
| Shows serologic IDENTITY for Blastomyces |
|
|
Term
1&4 - Known Blastomyces + serum (control)
2 - patient J. Jones serum
3 - patient D. Brown serum
4 - Known Blastomyces AGN
It may be concluded that the patient in well #5:
[image] |
|
Definition
| Does NOT have Blastomyces dermatitidis |
|
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Term
In performing a cytomegalovirus (CMV) ABY (using a latex agglutination method) test the following results were obtained:
+ Control: Clumping
= Control: No Clumping
Patient Specimen: Clumping
These results indicate: |
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Definition
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Term
A 50-year-old teacher began complaining of weakness, fatigue, and low back pain. Lytic bone lesions were observed in her X-rays. The following immunoglobulin levels were obtained:
Decrease IgA
High Increase IgG
Decrease IgM
A likely Diagnosis is: |
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Definition
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Term
The following results were obtained when a beta-HCG test (agglutination inhibition method) was performed on a urine specimen:
+ Control: No Clumping
= Control: Clumping
Patient Specimen: No Clumping
The technologist should report the test as: |
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Definition
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Term
Listed below are the steps used in fluorescent antibody microscopy.
A. Anti-rabbit antibody tagged with fluorescein added to slide; incubation followed by rinse.
B. Patient serum placed onto slide; incubation followed by rinse.
C. Slide examined using fluorescent microscope.
D. Patient specimen fixed onto slide.
E. Anti-human antibody tagged with fluorescein added to slide; incubation followed by rinse.
F. Fluorescence indicates Legionella antigen present.
G. Fluorescence indicates Legionella antibody present.
H. Known Legionella organism fixed onto slide.
I. Anti-Legionella antibody tagged with fluorescein placed onto slide; incubation followed by rinse.
J. Anti-Legionella antibody (made by rabbits) placed onto slide; incubation followed by rinse.
The correct order for the "Direct Fluorescent Assay" test for Legionella is:
The correct order for the "Direct Fluorescent Assay" test for Legionella is: |
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Definition
|
|
Term
| The type of cell most often associated with acute inflammation is: |
|
Definition
| Polymorphonuclear neutrophils (PMNs) |
|
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Term
| The term used to describe all ABYs of the IgM type is |
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Definition
|
|
Term
| The immunoglobulin that has a secretory piece is: |
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Definition
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Term
Listed below are the sequence of events for Enzyme Immunoassays (EIA) - in no particular order. Incubation, wash, and certrifugation steps have been omitted.
A. Enzyme substrate added and amount of color measured.
B. Specific antibody attached to a solid phase.
C. Specific antigen attached to a solid phase.
D. Patient specimen added.
E. Enzyme-labeled anti-human antibody added.
F. Mix patient specimen with labeled antibody.
G. Enzyme-labeled antibody (against antigen) added.
H. Add excess unlabeled antigen (attached to solid phase).
I. Patient specimen and labeled antigen added to tube.
The correct order for an indirect ELISA test is: |
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Definition
|
|
Term
Match the immunoglobulin with its characteristic:
The Largest Immunoglobulin |
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Definition
|
|
Term
Match the immunoglobulin with its characteristic:
Associate with mucous membranes |
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Definition
|
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Term
Match the immunoglobulin with its characteristic:
Immunoglobulin with the highest serum concentration |
|
Definition
|
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Term
Match the immunoglobulin with its characteristic:
Immunoglobulin that most effectively binds complement |
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Definition
|
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Term
Match the immunoglobulin with its characteristic:
Associated with hay fever |
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Definition
|
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Term
Match the immunoglobulin with its characteristic:
Reacts best at 30 degrees to 37 degrees Celsius |
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Definition
|
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Term
Match the immunoglobulin with its characteristic:
Immunoglobulin present in Waldenstrom's macroglobulinemia |
|
Definition
|
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Term
Match the immunoglobulin with its characteristic:
Found with IgM on mature B cells |
|
Definition
|
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Term
Indicate whether the following is characteristic of monoclonal or polyclonal ABYs:
Produced by many different kinds of plasma cells |
|
Definition
|
|
Term
Indicate whether the following is characteristic of monoclonal or polyclonal ABYs:
Heterogenous group of ABYs |
|
Definition
|
|
Term
Indicate whether the following is characteristic of monoclonal or polyclonal ABYs:
Specific for a single epitope |
|
Definition
|
|
Term
Indicate whether the following is characteristic of monoclonal or polyclonal ABYs:
Problems with cross-reactivity |
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Definition
|
|
Term
T or F:
Individuals who lack IgA are prone to anaphylactic reactions when given blood transfusions |
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Definition
|
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Term
T or F:
In nephelometry the light device is placed in a direct line with the incident light |
|
Definition
|
|
Term
T or F:
Agglutination is the combination of PARTICULATE antigens with antibody to produce clumps |
|
Definition
|
|
Term
T or F:
The bone marrow is primary lymphoid tissue. |
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Definition
|
|
Term
T or F:
The term "sensitization" refers to initial immunoglobulin binding |
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Definition
|
|
Term
T or F:
Rheumatoid factor is associated with false positive results in reverse passive agglutination tests |
|
Definition
|
|
Term
T or F:
Flow cytometry can be used to detect specific cell AGNs |
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Definition
|
|
Term
T or F:
Nucleic acid probes detect a specific nucleic acid sequence. |
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Definition
|
|
Term
T or F:
HLA class I antigens are found on B cells. |
|
Definition
|
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Term
T or F:
Infants at birth usually have adult levels of IgG. |
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Definition
|
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Term
T or F:
Acute phase reactants usually DECREASE when an injury occurs. |
|
Definition
|
|
Term
T or F:
Immunoglobulins are NOT immunogens. |
|
Definition
|
|
Term
T or F:
Antibody responses to protein are usually T cell INDEPENDENT. |
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Definition
|
|
Term
T or F:
The bone marrow is the site of antigen-DEPENDENT lymphopoiesis. |
|
Definition
|
|
Term
T or F:
Cytotoxic T cells interact with Class II bound antigen to remove viral infected cells. |
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Definition
|
|
Term
T or F:
The skin is an example of the external defense system. |
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Definition
|
|
Term
T or F:
Human IgA has 2 subtypes |
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Definition
|
|
Term
T or F:
T helper cells help B cells switch the ABY class produced |
|
Definition
|
|
Term
T or F:
The normal ratio of kappa to lambda chains is 1:2 |
|
Definition
|
|
Term
T or F:
Homogeneous immunoassays do NOT require a separation step |
|
Definition
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Term
The following HLA results were obtained:
Mother: A1/A19, B5/B37, Cw1/Cw11, DR2/DR7
Man A: A3/A28, B14/B35, Cw5/Cw7, DR3/DR9
Man B: A1/A31, B12/B37, Cw2/Cw5, DR12/DR18
Child: A1/A7, B5/B37, Cw1/Cw7,
DR2/DR3
Based on these results, which man is the father? |
|
Definition
| Neither Man is the father |
|
|
Term
The following HLA results were obtained:
Mother: A1/A19, B5/B37, Cw1/Cw11, DR2/DR7
Man A: A3/A28, B14/B35, Cw5/Cw7, DR3/DR9
Man B: A1/A31, B12/B37, Cw2/Cw5, DR12/DR18
Child: A1/A7, B5/B37, Cw1/Cw7,
DR2/DR3
What is one of the haplotypes that belongs to the child? |
|
Definition
|
|
Term
Match the disease with its immunodeficiency classification:
Chronic granulomatus disease |
|
Definition
| Neutrophil function defect |
|
|
Term
Match the disease with its immunodeficiency classification:
Bruton's agammaglobulinemia |
|
Definition
| Primary deficiency of B cell system |
|
|
Term
Match the disease with its immunodeficiency classification:
Severe combined immunodeficiency |
|
Definition
| Combined deficiency of cellular and humoral immunity |
|
|
Term
Match the disease with its immunodeficiency classification:
Diabetes mellitus |
|
Definition
| Secondary immunodeficiency |
|
|
Term
Match the disease with its immunodeficiency classification:
Common variable immunodeficiency |
|
Definition
| Primary deficiency of B cell system |
|
|
Term
Match the disease with its immunodeficiency classification:
DiGeorge Anomaly |
|
Definition
| Primary deficiency of cellular immunity |
|
|
Term
Classify the following as Class I or II:
Located on gene loci: DR, DQ, DP |
|
Definition
|
|
Term
Classify the following as Class I or II:
Expressed on all nucleated cells |
|
Definition
|
|
Term
Classify the following as Class I or II:
Used by AGN presenting cells to present AGN to T helper cells |
|
Definition
|
|
Term
The following test was performed:
Known ABY and known AGN tagged with enzyme are placed in patient sample (unknown AGN)
The amount of enzymatic activity is determined.
This test is the: |
|
Definition
|
|
Term
| Markers which must be on a thymocyte so it can be called a double-positive cell are: |
|
Definition
|
|
Term
| An immunoglobulin which possesses a J chain is: |
|
Definition
|
|
Term
Identify the regions indicated on the immunoglobulin molecule diagram:
[image]
Fab fragment |
|
Definition
|
|
Term
Identify the regions indicated on the immunoglobulin molecule diagram:
[image]
Fc fragment |
|
Definition
|
|
Term
Identify the regions indicated on the immunoglobulin molecule diagram:
[image]
Heavy chain |
|
Definition
|
|
Term
Identify the regions indicated on the immunoglobulin molecule diagram:
[image]
Light chain |
|
Definition
|
|
Term
Identify the regions indicated on the immunoglobulin molecule diagram:
[image]
Hinge region |
|
Definition
|
|
Term
Total WBC = 4,000/ microliter
Basophils 1%
Eosinophils 4%
Neutrophils 45%
Lymphocytes 40%
Monocytes 10%
CD3 70%
CD4 25%
CD8 45%
CD20 10%
CD56 20%
CD45 99%
The ratio of Helpter to Cytotoxic T cells is: |
|
Definition
|
|
Term
Total WBC = 4,000/ microliter
Basophils 1%
Eosinophils 4%
Neutrophils 45%
Lymphocytes 40%
Monocytes 10%
CD3 70%
CD4 25%
CD8 45%
CD20 10%
CD56 20%
CD45 99%
The cytotoxic T cell count is: |
|
Definition
|
|
Term
Total WBC = 6,000/ microliter
Basophils 1%
Eosinophils 4%
Neutrophils 45%
Lymphocytes 40%
Monocytes 10%
CD3 70%
CD4 25%
CD8 45%
CD20 10%
CD56 20%
CD45 99%
The absolute NK cell count is: |
|
Definition
|
|
Term
| The part of the ABY molecule that combines with an epitope is found in: |
|
Definition
| The variable regions of the heavy and light chains |
|
|
Term
The following serum immunofixation electrophoresis results was obtained on a woman. These results indicate the patient:
[image] |
|
Definition
| Has a IgG Kappa monoclonal gammopathy |
|
|
Term
This serum protein electrophoresis pattern was obtained on a 65-year old man. These results are consistent with:
[image] |
|
Definition
|
|
Term
What is the name of the following procedure:
[image] |
|
Definition
|
|
Term
What is the name of the following procedure:
[image] |
|
Definition
|
|
Term
What is the name of the following procedure:
[image] |
|
Definition
|
|
Term
What does this SPE pattern show?
[image] |
|
Definition
| "common variable immunodeficiency" |
|
|
Term
What does this SPE pattern show?
[image] |
|
Definition
|
|
Term
| Prepares AGN for phagocytosis |
|
Definition
|
|
Term
|
Definition
|
|
Term
| An excess amount of a particular immunoglobulin |
|
Definition
|
|
Term
| Free light chains in urine |
|
Definition
|
|
Term
| Proteins which precipitate at cold temperatures |
|
Definition
|
|
Term
| Small molecule capable of stimulating ABY formation when combined with a carrier molecule |
|
Definition
|
|
Term
| The SUM of all ABY-epitope interactions |
|
Definition
|
|
Term
| The ionic cloud surrounding RBC |
|
Definition
|
|
Term
| The ability of a particular ABY to bind to one AGN instead of another |
|
Definition
|
|
Term
| ABYs interacting with structurally similar AGNs |
|
Definition
|
|
Term
| When a cytokine acts on a nearby cell |
|
Definition
|
|
Term
| Different forms of a gene |
|
Definition
|
|
