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Exam 2
Parasitology- Introduction to Fecal Parasites
18
Microbiology
Graduate
03/22/2013

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Cards

Term
when do you collect fecal specimens?
Definition
alternate days
Term
what should you avoid in fecal specimens?
Definition
barium, sulfate, mineral oil, bismuth, non-absorbable anti-diarrheal preparations, anti-malarials and some anitbiotics
Term
what are the two collection schedules to follow?
Definition
3 specimens within 10 days or 6 specimens within 14 days
Term
Types of fresh specimens (3)
Definition
liquid, semi-formed, and formed
Term
Liquid specimen examination length
Definition
within 30 minutes of passage
Term
semi-formed specimen examination length
Definition
within 1 hour of passage
Term
formed specimen examination length
Definition
within 1 day of passage
Term
Fecal specimen preservatives (4)
Definition
PVA, Formalin, MIF, and SAF
Term
Smears for microscopic examination (3)
Definition
direct, concentration and stained
Term
Gastrointestional specimens (3)
Definition
sigmoidoscopy material, duodenal contents, and cellophane tape preparations
Term
Type of antigen detection (3)
Definition
DFA, EIA, and probes
Term
What is DFA used to detect?
Definition
Giardia and Crytoporidium
Term
What is EIA used to detect?
Definition
E. histolytica, Giardia, Cryptosporidum, and T. vaginalis
Term
What are probes used to detect?
Definition
T. vaginalis
Term
Which parasite is detect by antibodies?
Definition
E. histolytica
Term
Non-GI specimens (5)
Definition
Urogenital specimens, sputum, aspirates, spinal fluid, biopsy material
Term
Explain the ova & parasite test for stool parasites
Definition
The ova and parasite test for stool parasites include three separate protocols: the direct wet mount, the concentration, and the permanent stained smear. The wet prep detects motile protozoan trophozoites in fresh stool. The concentration detects protozoan cysts, coccidian oocysts, microsporidial spores and helminith eggs and larvae from sedimentation or flotation.
Term
What stains are used in O & P?
Definition
The permanent stain is to detect intestinal protozoa. The typical stains used are Wheatley modification of the Gomori tissue trichrome and the iron-hemotoxylin stains.
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