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Exam 2
Parasitology- Blood Parasites
24
Microbiology
Graduate
03/22/2013

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Term
preparation of thick blood smears
Definition
lysing the red blood cells with the edge of another slide, this is to determine the level of parasitemia.
Term
preparation of thin blood smears
Definition
prepared by doing a blood smear with feathered edge to obtain a monolayer of red cells.
Term
life cycle of Plasmodium spp.
Definition
The life cycle of Plasmodium spp. starts with the mosquito ingesting gametocytes from an infected human, there is exflagellation of microgametocytes and fertilization of macrogametocyte, formation of oocysts and formation of sporozoites then migration of sporozoites to salivary glands. The mosquito takes a blood meal and injects the infective sporozoites, the exoerythrcytic phase in which schizogony produce thousands of Merozoites, and then the Erythrocytic phase in which there is a production of gametocytes.
Term
clinical presentation of Plasmodium spp.
Definition
The clinical presentation of Plasmodium spp. is malaria, which appears when sporozoites are introduced into the human blood stream, causes a fever, chills, weakness, headache, vomiting, diarrhea, anemia, pulmonary and renal dysfunction, neurologic and changes
Term
list of the blood parasites
Definition
Plasmodium falciparum, P. vivax, P. malariae, P. Ovale, Babesia microti, Trypanosome rhodesiense, Trypanosome cruzi, Leishmania spp. Toxoplasmosis gondii
Term
how to ID P. falciparum
Definition
small, delicate rings with double chromatin dots, multiple rings/RBC, black pigment, 20 merozoites and a banana shaped gametocyte
Term
how to ID P. vivax
Definition
schuffners dots, enlarged RBCs, golden brown pigment, 16 merozoites
Term
how to ID P. malariae
Definition
dark blue cytoplasm, rounded and compact trophozoites, 8 merozoites
Term
how to ID P. ovale
Definition
schuffner’s dots and enlarged RBCs, dark blue cytoplasm, dark brown pigment, 8 merozoites
Term
how to ID Babesia microti
Definition
small ring trophozoites, tetrad formations within RBCs, can be observed extracellularly
Term
how to ID Trypanosoma rhodesiense
Definition
East African sleeping sickness, Tsetse fly vector
Term
how to ID Trypanosoma gambiense
Definition
West African sleeping sickness, Tsetse fly vector
Term
how to ID Trypanosoma cruzi
Definition
Chagas' disease, Reduviid bug vector, C or S shaped organism and crithidial forms in blood, large kinetoplast
Term
how to ID Leishmania spp.
Definition
Sand fly vector, diagnosis by skin biopsy, spleen, lymph node and liver
Term
how to ID Toxoplasmosis gondii
Definition
trophozoites found intracellular in all organs (histology), serology to detect antibody
Term
list the microfilariae
Definition
Wuchereria bancrofti, Brugi malayi, Loa loa, Onchoerca volvulus, Mansonella spp, Drancunculus medinensis
Term
how to ID Wuchereria bancrofti
Definition
sheath, nuclei do not extend to the tip of tail
Term
how to ID Brugi malayi
Definition
sheath, terminal nuclei separated
Term
how to ID Loa loa
Definition
sheath, nuclei extend to top of tail
Term
how to ID Onchoerca volvulus
Definition
no sheath, nuclei do not extend to tip of tail
Term
how to ID Mansonella ozzardi
Definition
no sheath, nuclei do not extend to tip of tail, adult worms in body cavity, microfilariae in blood and skin
Term
how to ID Drancunculus medinesis
Definition
skin lesions, larvae in water, copepod vector
Term
how to ID Mansonella perstans
Definition
adults cause mesentery, microfilariae in blood, no sheath, nuclei do not extend to blunt top of tail
Term
how to ID Mansonella streptocerca
Definition
adults in subcutaneous tissues, microfilariae in skin, no sheath nuclei extend to tip of hooked tail
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